Mechanisms of hemolysis caused by anti-erythrocyte antibodies in the pathological condition of canine babesiosis.

• Project/Area Number: 20688014
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Clinical veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: YAMASAKI Masahiro Hokkaido University, 大学院・獣医学研究科, 准教授 (40322846)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: イヌバベシア症 / 抗赤血球抗体 / heat shock protein 70 / バベシア原虫 / 貧血 / Heat shock protein 70

Research Abstract

In canine babesiosis, antibodies that reacted with canine heat shock protein 70 (hsp70) were detected in dog sera. Because hsp70 from Babesia parasites showed high homology with canine hsp70, anti-Babesia parasite hsp70 antibody might cross-react with canine hsp70. Since canine hsp70 was expressed in canine erythrocytes, it was possible that anti-hsp70 antibodies in dog sera would be anti-erythrocyte membrane antibodeis ; however, canine hsp70 was not found on the membrane surface of erythrocytes, suggesting that erythrocytes would not be targets of anti-hsp70 antibody.

Research Products

• [Journal Article] Detection of anti-Babesia gibsoni heat shock protein 70 antibody and anti-canine heat shock protein 70 antibody in sera from Babesia gibsoni-infected dogs. (2011)
  • Author(s): Masahiro Yamasaki, Mikiko Ishida, Kensuke Nakamura, Noboru Sasaki, Masahiro Murakami, Wickramasekara Rajapakshage Bandula Kumara, Yu Tamura, Sue Yee Lim, Hiroshi Ohta, Mitsuyoshi Takiguchi
  • Journal Title: Veterinary Parasitology (in press)
  • NAID: 120003388806
  • Peer Reviewed
• [Journal Article] Detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis. (2011)
  • Author(s): Masahiro Yamasaki, Yusuke Kobayashi, Kensuke Nakamura, Noboru Sasaki, Masahiro Murakami, Wickramasekara Rajapakshage Bandula Kumara, Hiroshi Ohta, Osamu Yamato, Yoshimitsu Maede, Mitsuyoshi Takiguchi, Babesia gibsoni
  • Journal Title: Experimental Parasitoloty 127巻 Pages: 119-126
  • Peer Reviewed
• [Journal Article] Babesia gibsoni Detection in blood smears and formalin-fixed, paraffin-embedded tissues using deoxyribonucleic acid in situ hybridization analysis. (2011)
  • Author(s): Yamasaki M
  • Journal Title: Experimental Parasitoloty Volume: 127 Pages: 119-126
  • NAID: 120003388804
  • Peer Reviewed
• [Journal Article] Reduced transcript levels of the heat shock protein 70 gene in diminazene aceturate-resistant Babesia gibsoni variants under low concentrations of diminazene aceturate. (2010)
  • Author(s): Shiang-Jyi Hwang, Masahiro Yamasaki, Kensuke Nakamura, Noboru Sasaki, Masahiro Murakami, Wickramasekara Rajapakshage Bandula Kumara, Hiroshi Ohta, Yoshimitsu Maede, Mitsuyoshi Takiguchi
  • Journal Title: Japanese Journal of Veterinary Research 58巻 Pages: 155-164
  • NAID: 120002548795
  • Peer Reviewed
• [Journal Article] Development and characterization of a strain of Babesia gibsoni resistant to diminazene aceturate in vitro. (2010)
  • Author(s): Shiang-Jyi Hwang, Masahiro Yamasaki, Kensuke Nakamura, Noboru Sasaki, Masahiro Murakami, Wickramasekara Rajapakshage Bandula Kumara, Hiroshi Ohta, Yoshimitsu Maede, Mitsuyoshi Takiguchi
  • Journal Title: Journal of Veterinary Medical Science 72巻 Pages: 765-771
  • NAID: 130000168031
  • Peer Reviewed
• [Journal Article] Development and characterization of a strain of Babesia gibsoni resistant to diminazene aceturate in vitro. (2010)
  • Author(s): Hwang S-J
  • Journal Title: Journal of Veterinary Medical Science Volume: 72(6) Pages: 765-761
  • Peer Reviewed
• [Journal Article] Reduced transcript levels of the heat shock protein 70 gene in diminazene aceturate-resistant Babesia gibsoni variants under low concentrations of diminazene aceturate. (2010)
  • Author(s): Hwang S-J
  • Journal Title: Japanese Journal of Veterinary Research Volume: 58(3-4) Pages: 155-164
  • Peer Reviewed
• [Journal Article] Contrast-enhanced ultrasonography for characterization of canine focal liver lesions. (2010)
  • Author(s): Nakamura K
  • Journal Title: Veterinary Radiology & Ultrasound Volume: 51(1) Pages: 79-85
  • Peer Reviewed
• [Journal Article] Contrast-enhanced ultrasonography for characterization of focal splenic lesions in dogs. (2010)
  • Author(s): Nakamura K
  • Journal Title: Journal of Veterinary Internal Medicine Volume: 24 Pages: 1290-1297
  • Peer Reviewed
• [Journal Article] Effects and mechanisms of action of ionophorous antibiotics valinomycin and salinomycin-Na on Babesia gibsoni in vitro. (2009)
  • Author(s): Masahiro Yamasaki, Kensuke Nakamura, Norihisa Tamura, Shiang-Jyi Hwang, Muneyoshi Yoshikawa, Noboru Sasaki, Hiroshi Ohta, Osamu Yamato, Yoshimitsu Maede, Mitsuyoshi Takiguchi
  • Journal Title: Journal of Parasitology 95巻 Pages: 1532-1538
  • Peer Reviewed
• [Journal Article] Isolation of antibabesial compounds from Brucea javanica, Curcuma xanthorrhiza, and Excoecaria cochinchinensis. (2009)
  • Author(s): Kazuaki Yamada, Subeki, Kensuke Nabeta, Masahiro Yamasaki, Ken Katakura, Hideyuki Matsuura
  • Journal Title: Bioscience, biotechnology, and biochemistry 73巻 Pages: 776-780
  • NAID: 10027539316
  • Peer Reviewed
• [Journal Article] Evaluation of efficary of Bruceine A, a natural quassinoid compound extracted from a medicinal plant, Brucea javanica, for canine babesiosis. (2009)
  • Author(s): Ryo Nakao, Chiaki Mizukami, Yuta Kawamura, Subeki, Saw Bawm, Masahiro Yamasaki, Yoshimitsu Maede, Hideyuki Matsuura, Kensuke Nabeta, Nariaki Nonaka, Yuzaburo Oku, Ken Katakura
  • Journal Title: Journal of Veterinary Medical Science 71巻 Pages: 33-41
  • Peer Reviewed
• [Journal Article] Effects and mechanisms of action of ionophorous antibiotics valinomycin and salinomycin-Na on Babesia gibsoni in vitro (2009)
  • Author(s): Yamasaki, M., Nakamura, K., Tamura, N., Hwang, S.J., Yoshikawa, M., Sasaki, N., Ohta, H., Yamato, O., Maede, Y., Takiguchi, M.
  • Journal Title: J.Parasitol. 95 Pages: 1532-1538
  • Peer Reviewed
• [Journal Article] Isolation of antibabesial compounds from Brucea javanica, Curcuma xanthorrhiza, and Excoecaria cohinchinensis (2009)
  • Author(s): Yamada, K., Subeki, Nabeta, K., Yamasaki, M., Katakura, K., Matsuura, H.
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 776-780
  • Peer Reviewed
• [Journal Article] Evaluation of efficacy of Bruceine A, a natural quassinoid compound extracted from a medicinal plant, Brucea javanica, for canine babesiosis (2009)
  • Author(s): Nakao, R., Mizukami, C., Kawamura, Y., Subeki, Bawm, S., Yamasaki, Y., Maede, Y., Matsuura, H., Nabeta, K., Oku, Y., Katakura, K.
  • Journal Title: J.Vet.Med.Sci. 71 Pages: 33-41
  • NAID: 110007055383
  • Peer Reviewed
• [Journal Article] Evaluation of efficacy of Bruceine A, a natural quassinoid compound extracted from a medicinal plant, Brucea javanica, for caninebabesiosis (2009)
  • Author(s): Nakao, R., Mizukami, C., Kawamura, Y., Subeki, Bawm, S., Yamasaki, M., Maede, Y., Matsuura, H., Nabeta, K., Oku, Y., Katakura. K.
  • Journal Title: J. Vet. Med. Sci 71 Pages: 33-41
  • Peer Reviewed
• [Journal Article] In vitro evaluation of the growth inhibitory activities of 15 drugs against Babesia gibsoni (Aomori strain). (2008)
  • Author(s): Aya Matsuu, Masahiro Yamasaki, Xuenan Xuan, Hiromi Ikadai, Yoshiaki Hikasa
  • Journal Title: Veterinary Parasitology 157巻 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Flow cytometry to evaluate the level of Babesia gibsoni parasitemia in vivo and in vitro by using the fluorescent nucleic acid stain SYTO16. (2008)
  • Author(s): Masahiro Yamasaki, Shiang-Jyi Hwang, Hiroshi Ohta, Osamu Yamato, Yoshimitsu Maede, Mitsuyoshi Takiguchi
  • Journal Title: Japanese Journal of Veternary Research 55巻 Pages: 129-136
  • NAID: 120000951648
  • Peer Reviewed
• [Journal Article] Heat shock response of Babesia gibsoni heat shock protein 70. (2008)
  • Author(s): Masahiro Yamasaki, Motoshi Tajima, Osamu Yamato, Shiang-Jyi Hwang, Hiroshi Ohta, Yoshimitsu Maede
  • Journal Title: Journal of Parasitology 94巻 Pages: 119-124
  • Peer Reviewed
• [Journal Article] Retrospective Diagnosis of Feline GM2 Gangliosidosis Variant 0(Sandhoff-like Disease)in Japan : Possible Spread of the Mutant Allele in the Japanese Domestic Cat Population (2008)
  • Author(s): Yamato, O., Hayashi, D., Satoh, H., Shoda, T., Uchida, K., Nakayama, H., Sasaki, H., Masegi, T., Murai, A., Iida, T., Hisada, H., Hisada, A., Yamasaki, M., Maede, Y., Arai, T.
  • Journal Title: J. Vet. Med. Sci 70 Pages: 813-818
  • NAID: 130000446910
  • Peer Reviewed
• [Journal Article] In vitro evaluation of the growth inhibitory activities of 15 drugs against Babesia gibsoni (Aomori strain) (2008)
  • Author(s): Matsuu, A., Yamasaki, M., Xuan, X., Ikadai, H. Hikasa, Y.
  • Journal Title: Vet. Parasitol 157 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Flow cytometry to evaluate the level of Babesia gibsoni parasitemia in vivo and in vitro by using the fluorescent nucleic acid stain SYTO16 (2008)
  • Author(s): Yamaski, M., Hwang, S. J., Ohta, H., Yamato, O., Maede, Y., Takiguchi, M.
  • Journal Title: Jpn. J. Vet. Res. 155 Pages: 129-136
  • Peer Reviewed
• [Journal Article] Heat shock response of Babesia gibsoni heat shock protein 70 (2008)
  • Author(s): Yamasaki, M., Tajima, M., Yamato, Y., Hwang, S. J., Ohta, H., Maede, Y.
  • Journal Title: J. Parasitol 94 Pages: 119-124
  • Peer Reviewed
• [Presentation] アムホテリシンBによるイヌバベシア症の治療に関する研究 (2011)
  • Author(s): 山崎真大、黄香寂、W.R.Bandula Kumara、谷山祐介、坪井嘉啓、大田寛、滝口満喜
  • Organizer: 第151回日本獣医学会学術集会(東日本大震災のため、講演要旨集の発行をもって終了)
  • Place of Presentation: 東京都府中市、東京農工大学
  • Year and Date: 2011-03-31
• [Presentation] アムホテリシンBによるイヌバベシア症の治療に関する研究 (2011)
  • Author(s): 山崎真大
  • Organizer: 第151回日本獣医学会学術集会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-03-31
• [Presentation] 培養Babesia gibsoniの末梢血単核球への侵入と増殖の検討 (2010)
  • Author(s): 谷山祐介、山崎真大、黄香寂、大田寛、滝口満喜
  • Organizer: 第79回日本寄生虫学会大会
  • Place of Presentation: 北海道旭川市、旭川医科大学
  • Year and Date: 2010-05-20
• [Presentation] 培養Babesia gibsoniの末梢血単核球への侵入と増殖の検討 (2010)
  • Author(s): 谷山裕介
  • Organizer: 第79回日本寄生虫学会大会
  • Place of Presentation: 旭川市大雪クリスタルホール
  • Year and Date: 2010-05-20
• [Presentation] Localization of Babesia gibsoni heat shock protein 70 in the parasites and erythrocytes in vitro. (2009)
  • Author(s): M. Yamasaki, S. J. Hwang, H. Ohta, M. Takiguchi,
  • Organizer: 2009 Asian Meeting of Animal Medicine Specialities (AMAMS), Asian Veterinary Meeting
  • Place of Presentation: Grand Victoria otel Taipei, Taipei, Taiwan
  • Year and Date: 2009-12-12
• [Presentation] Localization of Babesia gibsoni heat shock protein 70 in the parasites and erythrocytes in vitro (2009)
  • Author(s): M.Yamasaki, S.J.Hwang, H.Ohta, M.Takiguchi
  • Organizer: Asian Meeting of Animal Medicine Specialties(AMAMS2009)
  • Place of Presentation: 台北(台湾)
  • Year and Date: 2009-12-12
• [Presentation] Diminazene aceturate耐性Babesia gibsoni株に対する他の抗バベシア原虫薬の効果 (2009)
  • Author(s): 黄香寂、谷山祐介、山崎真大、大田寛、滝口満喜
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取県鳥取市、鳥取大学
  • Year and Date: 2009-09-26
• [Presentation] 培養Babesia gibsoniのストレス環境下におけるheat shock protein 70の役割に関する研究 (2009)
  • Author(s): 山崎真大、黄香寂、大田寛、滝口満喜
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京都千代田区、法政大学市ヶ谷キャンパス外濠校舎
  • Year and Date: 2009-03-28
• [Presentation] 培養Babesia gibsoniのストレス環境下におけるheat shogk protein 70の役割に関する研究 (2009)
  • Author(s): 山崎真大、黄寂香、大田寛、滝口満喜
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 法政大学(東京都)
  • Year and Date: 2009-03-28
• [Presentation] ポリエン系抗生物質アムホテリシンBの抗Babesia gibsoni効果 (2008)
  • Author(s): 原田絵梨子、山崎真大、大田寛、滝口満喜
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎県宮崎市、宮崎大学
  • Year and Date: 2008-09-25
• [Presentation] Diminazene aceturate耐性Babesia gibsoni株の作成 (2008)
  • Author(s): 黄香寂、山崎真大、大田寛、滝口満喜
  • Organizer: 第146回日本獣医学会学術集会
  • Place of Presentation: 宮崎県宮崎市、宮崎大学
  • Year and Date: 2008-09-25