Construction of a novel taste sensor using the taste receptor protein of vertebrate origin

• Project/Area Number: 20688015
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: The University of Tokyo
• Principal Investigator: MISAKA Takumi The University of Tokyo, 大学院・農学生命科学研究科, 准教授 (40373196)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000); Fiscal Year 2009: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2008: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
• Keywords: 分子間相互作用 / 味覚 / 受容体 / 味物質 / センサー / 培養細胞 / 食品 / 遺伝子 / 生体分子

Research Abstract

We planned to construct a novel taste sensor using the taste receptor protein. The taste censor should be present a stable score to the tastants. To measure the sweetness, we generated a cell line that were stably expressing the human sweet taste receptor (hT1R2/hT1R3). The cell could be worked as a sensor for sweet taste to obtain the change of fluorescent intensities of the intracellular calcium indicatordye.

Research Products

• [Journal Article] Amiloride reduces the sweet taste intensity by inhibiting the human sweet taste receptor Biochem. (2010)
  • Author(s): Imada, T., Misaka, T., Fujiwara, S., Okada, S., Fukuda, Y., Abe, K.
  • Journal Title: Biophys. Res. Commun. (in press)
• [Journal Article] pH-dependent structural change of neoculin in special reference to its taste-modifying activity. (2009)
  • Author(s): Morita, Y., Nakajima, K., Iiduka, K., Terada, T., Shimizu-Ibuka, A., Ito, K., Koizumi, A., Asakura, T., Misaka, T., Abe, K.
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 2552-2555
• [Journal Article] Acetic acid activates PKD1L3-PKD2L1 channel-a candidate sour taste receptor. (2009)
  • Author(s): Ishii, S., Misaka, T., Kishi, M., Kaga, T., Ishimaru, Y., Abe, K.
  • Journal Title: Biochem. Biophys. Res. Commun. 385 Pages: 346-350
• [Journal Article] pH-Dependent inhibition of the human bitter taste receptor hTAS2R16 by a variety of acidic substances. (2009)
  • Author(s): Sakurai, T., Misaka, T., Nagai, T., Ishimaru, Y., Matsuo, S., Asakura, T., Abe, K.
  • Journal Title: J. Agric. Food Chem. 57 Pages: 2508-2514
• [Journal Article] pH-dependent structural change in neoculin with special reference to its taste-modifying activity (2009)
  • Author(s): Morita, Y., ら
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 2552-2555
  • Peer Reviewed
• [Journal Article] Acetic acid activates PKD1L3-PKD2L1 channel--a candidate sour taste receptor (2009)
  • Author(s): Ishii, S., ら
  • Journal Title: Biochem.Biophys.Res.Commun. 385 Pages: 346-350
  • Peer Reviewed
• [Journal Article] pH-Dependent inhibition of the human bitter taste receptor hTAS2R16 by a variety of acidic substances (2009)
  • Author(s): Sakurai T. et.al.
  • Journal Title: J. Agric. Food Chem 57 Pages: 2508-2514
  • Peer Reviewed
• [Presentation] ヒト甘味受容体発現細胞による甘味阻害剤の検索 (2010)
  • Author(s): 今田隆将、阿部啓子、三坂巧
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] ヒト甘味受容体安定発現細胞株の作製の試み (2009)
  • Author(s): 今田隆将、阿部啓子、三坂巧
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/biofunc/
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/biofunc/
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/biofunc/
• [Patent(Industrial Property Rights)] 甘味受容体発現コンストラクト、これを発現させた細胞体、及びその利用 (2009)
  • Inventor(s): 阿部啓子、三坂巧、今田隆将、藤原聡
  • Industrial Property Rights Holder: 東京大学、長谷川香料株式会社
  • Filing Date: 2009-12-02