Immunological and physiological analysis of the function and construction of stromal cell network in secondary lymphoid organ

• Project/Area Number: 20689005
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kansai Medical University
• Principal Investigator: KATAKAI Tomoya Kansai Medical University, 医学部, 講師 (00324682)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000); Fiscal Year 2009: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2008: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
• Keywords: 細胞間相互作用 / 細胞動態 / ストローマ細胞 / 組織微小環境 / 二次リンパ器官 / 免疫応答 / リンパ節 / 遣伝子 / 細胞・組織 / 生体分子 / 発生・分化 / 免疫学 / 遺伝子

Research Abstract

In the secondary lymphoid organs in which immune responses are efficiently induced, the network of mesenchymal stromal cells support tissue architecture and immune cells behaviors. However, the detailed functions of stromal cells are still largely unclear. Technical advances to investigate lymphoid stromal cells are also required.
We established a method to efficiently isolate stromal cell fraction from mouse lymph node and for the culture of the stromal cells to make a confluent monolayer. Using this technique, we observed that isolated primary lymphocytes efficiently migrated on the stromal monolayer. Moreover, We constructed three-dimensional network of primary stromal cells using supportive meshwork substrates in vitro. We also tried to develop an animal model for genetically modifying stromal cells in vivo.

Research Products

• [Journal Article] Rap1 controls lymphocyte adhesion cascade and interstitial migration within lymph nodes in RAPL-dependent and -independent manners (2010)
  • Author(s): Ebisuno Y., Katagiri K., Katakai T., Ueda Y., Nemoto T., Inada H., Nabekura, J., Okada T., Kannagi R., Tanaka T., Miyasaka M., Hogg N., Kinashi T.
  • Journal Title: Blood 115 Pages: 804-814
  • Peer Reviewed
• [Journal Article] Rap1 controls lymphocyte adhesion cascades and interstitial migration within lymph nodes in RAPL-dependent and -independent manners (2010)
  • Author(s): Ebisuno Y., Katagiri K., Katakai T., et al.
  • Journal Title: Blood 115 Pages: 804-814
  • Peer Reviewed
• [Journal Article] Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes (2009)
  • Author(s): Katagiri K., Katakai T., Ebisuno Y., Ueda Y., Okada T., Kinashi T.
  • Journal Title: EMBO J. 28 Pages: 1319-1331
  • Peer Reviewed
• [Journal Article] CXCL13 production by an established lymph node stromal cell via lymphotoxin-beta receptor engagement involves the cooperation of multiple signaling pathways (2009)
  • Author(s): Suto H., Katakai T., Sugai M., Kinashi T., Shimizu A.
  • Journal Title: Int. Immunol. 21 Pages: 467-476
  • Peer Reviewed
• [Journal Article] CXCL13 production by an established stromal cell line via lymphotoxin-beta receptor engagement involves the cooperation of multiple signaling pathways (2009)
  • Author(s): Suto H., Katakai T., et al.
  • Journal Title: Int.Immunol. 21 Pages: 467-476
  • Peer Reviewed
• [Journal Article] CXCL13 production by an established stromal cell line via lymphotoxin-beta receptor engagement involves the cooperation of multiple signaling pathways (2009)
  • Author(s): Suto H., Katakai T, et.al.
  • Journal Title: Int. Immunol 21 Pages: 467-476
  • Peer Reviewed
• [Journal Article] Organizer-like reticular stromal cell layer common adult secondary lymphoid organs (2008)
  • Author(s): Katakai T., Suto H., Sugai M., Gonda H., Togawa A., Suematsu S., Ebisuno Y., Katagiri K., Kinashi T., Shimizu A.
  • Journal Title: J. Immunol. 181 Pages: 6189-6200
  • Peer Reviewed
• [Journal Article] Undesired meeting of lymphocytes: organ -specific infiltration and the organization of ectopic lymphoid tissue in a murine experimental autoimmune gastritis (2008)
  • Author(s): Katakai T., Shimizu A.
  • Journal Title: Immunol. Lett. 118 Pages: 103-109
  • Peer Reviewed
• [Journal Article] Organizer-like reticular stromal layer common to adult secondary lymphoid organs. (2008)
  • Author(s): Katakai T., et.al.
  • Journal Title: J. Immunol 181 Pages: 6189-6200
  • Peer Reviewed
• [Journal Article] Undesired meeting of lymphocytes : organ-specific infiltration and the organization of ectopic lymphoid tissue in a murine experimental autoimmune gastritis (2008)
  • Author(s): Katakai T. and Shimizu A.
  • Journal Title: Immunol. Lett 118 Pages: 103-109
  • Peer Reviewed
• [Presentation] LFA-1 dependent adhesion to ICAM-1 contributes to high-velocity migration of primary T cells on stromal cells isolated from lymph nodes (2009)
  • Author(s): 片貝智哉, 他
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-02
• [Presentation] LFA-1-dependent adhesion to ICAM-1 contributes to high-velocity migration of primary T cells on stromal cells isolated from lymph nodes (2009)
  • Author(s): 片貝智哉, 他
  • Organizer: 第39回 日本免疫学会総会・学術集会
  • Place of Presentation: 日本免疫学会総会・学術集会大阪国際会議場
  • Year and Date: 2009-12-02
• [Presentation] Lymph node derived stromal cell lines support the motility of lymphocytes in vitro (2008)
  • Author(s): 片貝智哉, 他
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都・国際会館
  • Year and Date: 2008-12-01
• [Presentation] Lymph node derived stromal cell lines support the motility of lymphocytes in vitro (2008)
  • Author(s): 片貝智哉, 他
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Remarks]
  • URL: http://www3.kmu.ac.jp/molgent/index.html