Examination of chemokine roles in CLP-induced sepsis model and its application for forensic diagnosis

• Project/Area Number: 20689015
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Legal medicine
• Research Institution: Wakayama Medical University
• Principal Investigator: ISHIDA Yuko 和歌山県立医科大学, 医学部, 講師 (10364077)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥25,870,000 (Direct Cost: ¥19,900,000、Indirect Cost: ¥5,970,000); Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2008: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
• Keywords: 敗血症 / 法医診断学 / ケモカイン / マウス / cecal ligation and puncture(CLP) / cecal ligation puncture / cecal ligation and puncture / ケモカイーン

Research Abstract

We examined CCR2 and CX3CR1 expression in sepsis-induced lung injury. Morphometrically, the average of CCR2-and CX3CR1-positive macrophages was significantly increased in sepsis group compared with control group in double-color immunofluorescence analysis. Immunohistochemical detection of CCR2 and CX3CR1 expression in the lungs can be considered as valuable diagnostic tools for the postmortem diagnosis of sepsis.

Research Products

• [Journal Article] 侵襲と生体反応～サイトカイン・ケモカインの病態生理学的役割～ (2012)
  • Author(s): 石田裕子
  • Journal Title: 和歌山医学 Volume: 63 Pages: 7-14
  • NAID: 40019277890
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of CCR2 and CX3CR1 in sepsis-induced lung injury (2009)
  • Author(s): An JL, Ishida Y, Kimura A, Tsokos M, Kondo T
  • Journal Title: Forensic Sci Int Volume: 192
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of CCR2 and CX3CR1 in sepsis-induced lung injury. (2009)
  • Author(s): An JL, Ishida Y, Kimura A, Tsokos M, Kondo T.
  • Journal Title: Forensic Sci Int. 192(1-3)
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of CCR2 and CX3CR1 in sepsis-induced lung injury (2009)
  • Author(s): An JunLing
  • Journal Title: Forensic Sci Int 192
  • Peer Reviewed
• [Journal Article] Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis (2008)
  • Author(s): Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T
  • Journal Title: J Immunol Volume: 181 Pages: 4208-18
  • Peer Reviewed
• [Presentation] マウス敗血症性腹膜炎におけるCX3CL1-CX3CR1の病態生理学的役割解析 (2008)
  • Author(s): 石田裕子, 林敬人, 木村章彦, 向田直史, 近藤稔和
  • Organizer: 第29回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] マウス敗血症性腹膜炎におけるCX3CH-CX3CRIの病態生理学的役割解析 (2008)
  • Author(s): 石田裕子
  • Organizer: 第29回日本炎症・再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] Immunohistochemical detection of CX3CR1 and CCR2 in sepsis-induced lung injury (2008)
  • Author(s): An JL, Ishida Y, Nosaka M, Kawaguchi M, Kimura A, Tsokos M, Kondo T
  • Organizer: 7^<th> International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka