Analysis of Sec63 conditional knockout mice

• Project/Area Number: 20689017
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Hokkaido University
• Principal Investigator: NISHIO Saori Hokkaido University, 北海道大学病院, 助教 (90463736)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000); Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2009: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2008: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: 常染色体優性多発性嚢胞腎 / 常染色体優性遺伝性肝嚢胞 / Sec63 / アポトーシス / 細胞増殖 / cilia / 水腎症 / 常染色体優性多発性肝嚢胞 / Pkd2 / Pkd1

Research Abstract

I elucidated the mechanism of cystformation in autosomal dominant polycystic liver disease by analysis of Sec63 conditional knockout mice. I also elucidated one of the function of Sec63. Sec63 is strongly associated with apoptosis.

Research Products

• [Journal Article] Distinct macrophage phenotypes contribute to kidney injury and repair. (2011)
  • Author(s): Lee S, Huen S, Nishio H, Nishio S, 他
  • Journal Title: J Am Soc Nephrol. 22(2) Pages: 317-326
  • Peer Reviewed
• [Journal Article] Activating AMP-activated protein kinase (AMPK) slows renal cystogenesis. (2011)
  • Author(s): Takiar V, Nishio S, 他
  • Journal Title: Proc Natl Acad Sci USA. 108(6) Pages: 2462-2467
  • Peer Reviewed
• [Journal Article] Transplantation-associated thrombotic microangiopathy aftersteroid pulse for polyserositis related to graft-versus-host disease. (2011)
  • Author(s): Ishikawa Y, Nishio S, 他
  • Journal Title: Clin Exp Nephrol. 15(1) Pages: 179-183
  • Peer Reviewed
• [Journal Article] Distinct macrophage phenotypes contribute to kidney injury and repair. (2011)
  • Author(s): Lee S, Huen S, Nishio H, Nishio S, 他
  • Journal Title: J Am Soc Nephrol. Volume: 22 Pages: 317-326
  • Peer Reviewed
• [Journal Article] Activating AMP-activated protein kinase (AMPK) slows renal cystogenesis. (2011)
  • Author(s): Takiar V, Nishio S, 他
  • Journal Title: Proc Natl Acad Sci Volume: 108 Pages: 2462-2767
  • Peer Reviewed
• [Journal Article] Transplantation-associated thrombotic microangiopathy after steroid pulse for polyserositis related to graft-versus-host disease. (2011)
  • Author(s): Ishikawa Y, Nishio S, 他
  • Journal Title: Clin Exp Nephrol. Volume: 15 Pages: 179-183
  • Peer Reviewed
• [Journal Article] Loss of oriented cell division does not initiate cyst formation. (2010)
  • Author(s): Nishio S, 他
  • Journal Title: J Am Soc Nephrol. 21(2) Pages: 295-302
  • Peer Reviewed
• [Journal Article] A kelch family protein Nd1-L functions as a metastasis suppressor in cancer cells via Rho family proteins mediated mechanism. (2010)
  • Author(s): Ohta Y, Fujimura L, Nishio S, 他
  • Journal Title: Int.J.Oncology. 36(2) Pages: 427-434
  • Peer Reviewed
• [Journal Article] Loss of oriented cell division does not initiate cyst formation. (2010)
  • Author(s): Nishio S
  • Journal Title: J Am Soc Nephrol. Volume: 21 Pages: 295-302
  • Peer Reviewed
• [Journal Article] A kelch family protein Ndl-L functions as a metastasis suppressor in cancer cells via Rho family proteins mediated mechanism. (2010)
  • Author(s): Ohta Y, Fujimura L, Nishio S, 他
  • Journal Title: Int.J.Oncology. Volume: 36 Pages: 427-434
  • Peer Reviewed
• [Journal Article] A murine model f neonatal diabetes mellitus in Glis3-deficent mice (2009)
  • Author(s): Watanabe N, Hiramatsu K, Miyamoto R, Yasuda K, Suzuki N, Oshima N, Kiyonari H, Shiba D, Nishio S, 他
  • Journal Title: FEBS Lett. 583(12) Pages: 2108-2113
  • Peer Reviewed
• [Journal Article] A murin model of neonatal diabetes mellitus in Glis3-deficent mice (2009)
  • Author(s): Watanabe N, Hiramatsu K, Miyamoto R, Yasuda K, Suzuki N, Oshima N, Kiyonari H, Shiba D, Nishio S, Mochizuki T, Yokoyama T, Maruyama S, Matsuo S, Wakamatsu Y, Hashimoto H.
  • Journal Title: FEBS Lett. 583 Pages: 2108-2113
  • Peer Reviewed
• [Journal Article] A kelch family protein Nd1-L functions as a metastasis suppressor in cancer cells via Rho family proteins mediated mechanism (2009)
  • Author(s): Ohta Y, Fujimura L, Nishio S, Arima M, Sakamoto A, Shimada H, Ochiai T, Tokuhisa T, Hatano M.
  • Journal Title: Int J Oncol 36 Pages: 427-434
  • Peer Reviewed
• [Journal Article] Loss of oriented cell division does not initiate cyst formation (2009)
  • Author(s): Nishio S, Tian X, Gallagher AR, Yu Z, Patel V, Igarashi P, Somlo S
  • Journal Title: J Am Soc Nephrol. 21 Pages: 295-302
  • Peer Reviewed
• [Journal Article] Cyst formation and activation of the extracellular regulated kinase pathway after kidney specific inactivation of Pkd1. (2008)
  • Author(s): Shibazaki S, Yu Z, Nishio S, 他
  • Journal Title: Hum Mol Genet. 17(11) Pages: 1505-1516
  • Peer Reviewed
• [Journal Article] Cyst formation and activation of the extracellular regulated kinase pathway after kidney specific inactivation of Pkdl (2008)
  • Author(s): Shibazaki S, Yu Z, Nishio S,Tian X, Brent T, Mitobe M, Louvi A, Ve lazquez H, Ishibe S, Cantley G, Ig arashi P, Somlo S
  • Journal Title: Human Moleculer Genetics 17 Pages: 1505-1516
  • Peer Reviewed
• [Presentation] 多発性嚢胞腎の嚢胞形成機序における細胞内細胞極性の関与の検討 (2010)
  • Author(s): 西尾妙織, 他
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-06-18
• [Presentation] 欠失変異体を用いた常染色体優性多発性嚢胞腎モデルメダカの解析 (2010)
  • Author(s): 石川康暢, 西尾妙織, 他
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-06-17
• [Presentation] 網膜症を認めず腎生検にて糖尿病性腎症と診断された2例の検討 (2009)
  • Author(s): 西尾妙織, 他
  • Organizer: 第43回日本糖尿病学会
  • Place of Presentation: 旭川市
  • Year and Date: 2009-11-08
• [Presentation] 網膜症を認めず腎生検にて糖尿病性腎症と診断された2例の検討 (2009)
  • Author(s): 西尾妙織, 三好秀明, 近藤琢磨, 永井聡, 吉岡成人, 小池隆夫
  • Organizer: 第43回日本糖尿病学会北海道地方会
  • Place of Presentation: 旭川市
  • Year and Date: 2009-11-08
• [Presentation] Interferon-gamma as a Key Cytokine in the Progression of Diabetic Nephropathy (2009)
  • Author(s): Ito M, Nishio S, Mochizuki T, Koike T
  • Organizer: 42st American Society of Nephrology annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-10-30
• [Presentation] Raf blockade ameliorates cyst progression and maintains kidney function in anorthologous mouse model of autosomal dominant polycystickidney (2009)
  • Author(s): Nishio S, Yu Z, Ming Ma, Ibrahim P, Zhang J, Tsai J, Bollag G, Somlo S
  • Organizer: 42st American Society of Nephrology annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-10-29
• [Presentation] Dissociation of oriented cell division and cyst formation in polycystic kidney disease (2009)
  • Author(s): Nishio S, Gallagher AR, Tian X, Yu Z, Igarashi P, Somlo S
  • Organizer: 42st American Society of Nephrology annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-10-29
• [Presentation] Predictive Marker and Treatment for Bone Loss after Renal Graft Trans plantation (2009)
  • Author(s): Nakazawa D, Ito M, Nishio S, Mochizuki T, Shimoda N, Miura M, Nonomura K, Koike T
  • Organizer: 42st American Society of Nephrology annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-10-29
• [Presentation] 無事に出産するに至った著明なネフローゼ症候群を呈した2型糖尿病合併妊娠の一例 (2009)
  • Author(s): 西尾妙織, 他
  • Organizer: 第252回日本内科学会地方会
  • Place of Presentation: 旭川市
  • Year and Date: 2009-09-12
• [Presentation] Sec63コンディショナルノックアウトマウスの解析 (2009)
  • Author(s): 西尾妙織, 望月俊雄, 三戸部倫大, 小池隆夫
  • Organizer: 第52回日本腎臓学会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-06-04
• [Presentation] Interferon-gamma as a key cytokine in the progression of diabetic nephropathy (2009)
  • Author(s): Ito M, Nishio S, 他
  • Organizer: 42th American Society of Nephrology annual meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Predictive Marker and Treatment for Bone Loss after Renal Graft Transplantation. (2009)
  • Author(s): Nakazawa D, Nishio S, 他
  • Organizer: 42th American Society of Nephrology annual meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Raf blockade ameliorates cyst progression and maintains kidney function in an orthologous mouse model of autosomal dominant polycystic kidney. (2009)
  • Author(s): Nishio S, 他
  • Organizer: 42st American Society of Nephrology annual meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Dissociation of oriented cell division and cyst formation in polycystic kidney disease. (2009)
  • Author(s): Nishio S, 他
  • Organizer: 42st American Society of Nephrology annual meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] 薬剤誘導型Pkd1コンディショナルノックアウトマウスに対するmTOR阻害薬の効果 (2009)
  • Author(s): 江端真一、西尾妙織, 他
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] Sec63コンディショナルノックアウトマウスの解析 (2009)
  • Author(s): 西尾妙織, 他
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] The Association of serum IgE concentration with prognosis of nephropathy in type II diabetes. (2009)
  • Author(s): Ito M, Nishio S, 他
  • Organizer: World Congress of nephrology 2009
  • Place of Presentation: Milan Italy
• [Presentation] Generation of Pkdl conditional knockout mice and treatment with everolimus (2008)
  • Author(s): Ebata S, Nishio S, et.al.
  • Organizer: アメリカ腎臓学会
  • Place of Presentation: 米国・フィラデルフィア
  • Year and Date: 2008-11-06
• [Presentation] 薬剤誘導性Pkd1コンディショナルノックアウトマウスの作成と解析 (2008)
  • Author(s): 江端真一、西尾妙織, 他
  • Organizer: 第51回日本腎臓学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-05-31
• [Presentation] Generation of Pkd1 conditional knockout mice and treatment with everolimus. (2008)
  • Author(s): Ebata S, Nishio S, 他
  • Organizer: 41th American Society of Nephrology annual meeting
  • Place of Presentation: Philadelphia, USA
• [Book] 嚢胞腎の腎腫大、「腎と透析」 (2008)
  • Author(s): 西尾妙織, 他
  • Publisher: 東京医学
• [Book] 腎と透析 (2008)
  • Author(s): 西尾妙織、望月俊雄
  • Publisher: 東京医学