Integrative genomic analyses for understanding the systematic gene expression changes in the brains of patients with psychiatric disorders

• Project/Area Number: 20689022
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Psychiatric science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: IWAMOTO Kazuya The Institute of Physical and Chemical Research, 医学部附属病院, 特任准教授 (40342753)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000); Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: エピジェネティクス / DNAメチレーション / 統合失調症 / 双極性障害 / 精神疾患 / 死後脳 / DNAメチル化 / 遺伝子発現

Research Abstract

We have profiled single nucleotide polymorphism pattern, copy number variations, and DNA methylation pattern in the postmortem brains of patients with mental disorders. We then linked the genetic/epigenetic variations to gene expression changes in the brain.

Research Products

• [Journal Article] Neurons show distinctive DNA methylation profile and higher interindividual variations compared with non-neurons. (2011)
  • Author(s): Iwamoto K, Bundo M, Ueda J, Oldham MC, Ukai W, Hashimoto E, Saito T, Geschwind DH, Kato T.
  • Journal Title: GenomeRes 21 Pages: 688-696
  • Peer Reviewed
• [Journal Article] Survey of the effect of genetic variations on gene expression in human prefrontal cortex and its application to genetics of psychiatric disorders. (2011)
  • Author(s): Iwamoto K, Ueda J, Bundo M, Kojima T, Kato T.
  • Journal Title: Neurosci Res 70 Pages: 238-242
  • Peer Reviewed
• [Journal Article] Comprehensive DNA methylation analysis of human peripheral blood leukocytes and lymphoblastoid cell lines. (2011)
  • Author(s): Sugawara H, Iwamoto K, Bundo M, Ueda J, Ishigooka J, Kato T.
  • Journal Title: Epigenetics 6 Pages: 508-515
  • Peer Reviewed
• [Journal Article] DNAメチル化研究の最新の動向 (2011)
  • Author(s): 岩本和也、文東美紀
  • Journal Title: 分子精神医学 11 Pages: 90-93
• [Journal Article] 神経細胞のDNAメチル化 (2011)
  • Author(s): 加藤忠史、文東美紀、岩本和也
  • Journal Title: 分子精神医学 11 Pages: 112-117
• [Journal Article] Neurons show distinctive DNA methylation profile and higher interindividual variations compared with non-neurons (2011)
  • Author(s): Kazuya Iwamoto
  • Journal Title: Genome Research Volume: (In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Survey of the effect of genetic variations on gene expression in human prefrontal cortex and its application to genetics of psychiatric disorders (2011)
  • Author(s): Kazuya Iwamoto
  • Journal Title: Neuroscience Research Volume: (In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Measuring RNA editing of serotonin 2C receptor (2011)
  • Author(s): Kazuya Iwamoto
  • Journal Title: Biochemistry (Moscow) Volume: (In press)(掲載確定)
• [Journal Article] Comprehensive DNA methylation analysis of human peripheral blood leukocytes and lymphoblastoid cell lines. (2011)
  • Author(s): Sugawara Hiroko
  • Journal Title: Epigenetics Volume: (In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Therapeutic implications of down-regulation of cyclophilin D in bipolar disorder. (2010)
  • Author(s): Kubota M, Kasahara T, Iwamoto K, Komori A, Miyauchi T, Ishiwata M, Kato T.
  • Journal Title: Int J Neuropsychopharmacol 13 Pages: 1355-1368
  • Peer Reviewed
• [Journal Article] Effect of mood stabilizers on gene expression in lymphoblastoid cells. (2010)
  • Author(s): Sugawara H, Iwamoto K, Bundo M, Ishiwata M, Ueda J, Kakiuchi C, Ishigooka J, Kato T.
  • Journal Title: J Neural Transm 117 Pages: 155-164
  • Peer Reviewed
• [Journal Article] Mutation screening and assessment of the effect of genetic variations on expression and RNA editing of serotonin receptor 2C in the human brain. (2010)
  • Author(s): Bundo M, Iwamoto K, Yamada K, Yoshikawa T, Kato T.
  • Journal Title: Psychiatry Clin Neurosci 64 Pages: 57-61
  • Peer Reviewed
• [Journal Article] セロトニン2C受容体 (2010)
  • Author(s): 文東美紀、岩本和也、加藤忠史
  • Journal Title: 生体の科学 61 Pages: 394-396
• [Journal Article] 気分障害患者の死後脳におけるオミックス研究 (2010)
  • Author(s): 文東美紀、岩本和也
  • Journal Title: Medical Bio 48 Pages: 46-51
• [Journal Article] Expression of mitochondrial complex I subunit gene NDUFV2 in the lymphoblastoid cells derived from patients with bipolar disorder and schizophrenia. (2009)
  • Author(s): Washizuka S, Iwamoto K, Kakiuchi C, Bundo M, Kato T.
  • Journal Title: Neurosci Res 63 Pages: 199-204
  • Peer Reviewed
• [Journal Article] A 3-bp deletion of mitochondrial DNA tRNALys observed in lymphoblastoid cells. (2009)
  • Author(s): Sugawara H, Iwamoto K, Bundo M, Kazuno AA, Nakano Y, Ishigooka J, Kato T.
  • Journal Title: J Hum Genet 54 Pages: 612-613
  • Peer Reviewed
• [Journal Article] 脳神経系ゲノムの多型性と精神疾患 (2009)
  • Author(s): 岩本和也、加藤忠史
  • Journal Title: 脳と精神の医学 20 Pages: 331-337
  • NAID: 10028262804
• [Journal Article] 双極性障害のゲノムワイド研究 (2009)
  • Author(s): 岩本和也、加藤忠史
  • Journal Title: 医学のあゆみ 231 Pages: 1019-1022
• [Journal Article] ゲノム多型の遺伝子発現への影響評価と精神疾患研究 (2009)
  • Author(s): 岩本和也、加藤忠史
  • Journal Title: 医学のあゆみ 229 Pages: 199-201
• [Journal Article] 精神疾患とepigenetics (2009)
  • Author(s): 加藤忠史、岩本和也
  • Journal Title: 細胞工学 28 Pages: 567-571
• [Journal Article] Serotonin receptor 2C and mental disorders : genetic, expression, and RNA editing studies (2009)
  • Author(s): 岩本和也
  • Journal Title: RNA biology 6 Pages: 248-253
  • Peer Reviewed
• [Journal Article] 双極性障害のゲノムワイド研究 (2009)
  • Author(s): 岩本和也
  • Journal Title: 医学のあゆみ 231 Pages: 1019-1022
• [Journal Article] ゲノム多型の遺伝子発現への影響評価と精神疾患研究 (2009)
  • Author(s): 岩本和也
  • Journal Title: 医学のあゆみ 229 Pages: 199-201
• [Journal Article] Gene expression profiling of major depression and suicide in the prefrontal cortex of postmortem brains. (2008)
  • Author(s): Tochigi M, Iwamoto K, Bundo M, Sasaki T, Kato N, Kato T.
  • Journal Title: Neurosci Res 60 Pages: 184-191
  • NAID: 10031136219
  • Peer Reviewed
• [Journal Article] Functional organization of the transcriptome in human brain. (2008)
  • Author(s): Oldham MC, Konopka G, Iwamoto K, Langfelder P, Kato T, Horvath S, Geschwind DH.
  • Journal Title: Nat Neurosci 11 Pages: 1271-1282
  • Peer Reviewed
• [Journal Article] Aberrant DNA methylation associated with bipolar disorder identified from discordant monozygotic twins. (2008)
  • Author(s): Kuratomi G, Iwamoto K, Bundo M, Kusumi I, Kato N, Iwata N, Ozaki N, Kato T.
  • Journal Title: Mol Psychiatry 13 Pages: 429-441
  • Peer Reviewed
• [Journal Article] Effect of a functional single nucleotide polymorphism in the 2',3'-cyclic nucleotide 3'-phosphodiesterase gene on the expression of oligodendrocyte-related genes in schizophrenia. (2008)
  • Author(s): Iwamoto K, Ueda J, Bundo M, Nakano Y, Kato T.
  • Journal Title: Psychiatry Clin Neurosci 62 Pages: 103-108
  • Peer Reviewed
• [Journal Article] Functional organization of the transcriptome in human brain. (2008)
  • Author(s): Michael C Oldham
  • Journal Title: Nature Neuroscience 11 Pages: 1271-1282
  • Peer Reviewed
• [Journal Article] Mutation screening and assessment of the effect of genetic variations on expression and RNA editing of serotonin receptor 2C in the human brain
  • Author(s): Bundo Miki
  • Journal Title: Psychiatry and Clinical Neurosciences (In press)
  • Peer Reviewed
• [Journal Article] 脳神経系ゲノムの多型性と精神疾患
  • Author(s): 岩本和也
  • Journal Title: 脳と精神の医学 (In press)
  • NAID: 10028262804
• [Journal Article] Epigenetic profiling in schizophrenia and related mental disorders
  • Author(s): Kazuya Iwamoto
  • Journal Title: Neuropsychobiology (in press)
  • Peer Reviewed
• [Journal Article] Expression of mitochondorial complex I subunit gene NDUFV2 in the lymphoblastoid cells derived from patients with bipolar disorder and schizophrenia
  • Author(s): Shinsuke Washizuka
  • Journal Title: Neuroscience Research (in press)
  • Peer Reviewed
• [Presentation] Epigenetics of bipolar disorder (2011)
  • Author(s): Kazuya Iwamoto
  • Organizer: Epigenetics in Clinical Medicine
  • Place of Presentation: Karolinska Institute, Sweden
  • Year and Date: 2011-04-04
• [Presentation] Epigenetics of bipolar disorder (2011)
  • Author(s): Iwamoto K, Kato T.
  • Organizer: Epigenetics in clinical Medicine
  • Place of Presentation: Stockholm, Sweden
• [Presentation] 脳ゲノムの多様性と精神疾患 (2011)
  • Author(s): 岩本和也、文東美紀、笠井清登、加藤忠史
  • Organizer: 第33回日本生物学的精神医学会
  • Place of Presentation: ホテルグランパシフィックLE DAIBA・東京
• [Presentation] Promoter-wide DNA methylation analysis in neuronal nuclei derived from brains of patients with psychiatric diseases (2010)
  • Author(s): Kazuya Iwamoto
  • Organizer: 60th American Society of Human Genetics
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2010-11-02
• [Presentation] 精神疾患患者由来試料を用いた分子病因およびバイオマーカー探索 (2010)
  • Author(s): 岩本和也
  • Organizer: 第18回日本精神行動遺伝医学会
  • Place of Presentation: 東北大学医学部・宮城
  • Year and Date: 2010-09-18
• [Presentation] 精神疾患患者由来試料を用いた分子病因およびバイオマーカー探索 (2010)
  • Author(s): 岩本和也
  • Organizer: 第18回日本精神行動遺伝医学会
  • Place of Presentation: 東北大学・宮城
  • Year and Date: 2010-09-18
• [Presentation] 精神疾患患者死後脳前頭葉神経細胞核におけるDNAメチル化解析 (2010)
  • Author(s): 岩本和也
  • Organizer: 第4回エピジェネティクス研究会
  • Place of Presentation: 米子市民文化ホール・鳥取
  • Year and Date: 2010-05-28
• [Presentation] Promoter-wide DNA methylation analysis in neuronal nuclei derived from brains of patients with psychiatric diseases. (2010)
  • Author(s): Iwamoto K, Bundo M, Ueda J, Kasai K, Kato T.
  • Organizer: 60th American Society of Human Genetics
  • Place of Presentation: Washington DC, USA
• [Presentation] Aberrant DNA methylation in neuronal nuclei derived from brains of patients with bipolar disorder. (2010)
  • Author(s): Iwamoto K, Bundo M, Ueda J, Kasai K, Kato T.
  • Organizer: 18th World Congress of Psychiatric Genetics
  • Place of Presentation: Athens, Greece
• [Presentation] エピジェネティック変異と精神疾患 (2010)
  • Author(s): 岩本和也、文東美紀、笠井清登、加藤忠史
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会
  • Place of Presentation: 神戸ポートアイランド・兵庫
• [Presentation] 精神疾患患者死後脳におけるエピゲノム解析 (2010)
  • Author(s): 岩本和也
  • Organizer: 第20回日本臨床精神薬理学会・第40回日本神経精神薬理学会
  • Place of Presentation: 仙台国際センター・宮城
• [Presentation] 精神疾患患者死後脳におけるDNAメチル化変異の探索 (2010)
  • Author(s): 岩本和也、文東美紀、上田順子、笠井清登、加藤忠史
  • Organizer: 第29回躁うつ病の薬理・生化学的研究懇話会
  • Place of Presentation: 箱根湯本・神奈川
• [Presentation] 精神疾患患者死後脳前頭葉神経細胞核におけるDNAメチル化解析 (2010)
  • Author(s): 岩本和也、文東美紀、上田順子、笠井清登、加藤忠史
  • Organizer: 第4回エピジェネティクス研究会
  • Place of Presentation: 米子市文化ホール・鳥取
• [Presentation] ヒト健常者大脳皮質単一神経細胞における染色体構造異常の検出 (2009)
  • Author(s): 岩本和也
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜市、パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] Comprehensive DNA methylation analysis in the human brain and its application to psychiatric diseases (2009)
  • Author(s): 岩本和也
  • Organizer: The 17th World Congress of Psychiatric Genetics
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-11-06
• [Presentation] Comprehensive DNA methylation analysis in neuronal and non-neuronal cells (2009)
  • Author(s): 岩本和也
  • Organizer: The 59th American Society for Human Genetics
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2009-10-23
• [Presentation] ヒト神経細胞・非神経細胞における網羅的DNAメチル化解析 (2009)
  • Author(s): 岩本和也
  • Organizer: 第3回エピジェネティクス研究会
  • Place of Presentation: 東京、学術総合センター
  • Year and Date: 2009-05-22
• [Presentation] ヒト脳神経系細胞におけるDNAメチル化解析-全ゲノムメチル化量の定量とメチル化領域の個人間差異の検討- (2009)
  • Author(s): 岩本和也
  • Organizer: 第31回日本生物学的精神医学会
  • Place of Presentation: 京都、国立京都国際会館
  • Year and Date: 2009-04-24
• [Presentation] ヒト脳神経細胞におけるDNAメチル化解析と精神疾患研究への応用について (2009)
  • Author(s): 岩本和也、加藤忠史
  • Organizer: 第5回行動遺伝研究会
  • Place of Presentation: 国立遺伝学研究所、三島
  • Year and Date: 2009-03-12
• [Presentation] ヒト健常者大脳皮質単一神経細胞における染色体構造異常の検出 (2009)
  • Author(s): 岩本和也、文東美紀、上田順子、中野陽子、足立直樹、鵜飼渉、橋本恵理、齋藤利和、小島俊男、加藤忠史
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜・神奈川
• [Presentation] ヒト神経細胞・非神経細胞における網羅的DNAメチル化解析 (2009)
  • Author(s): 岩本和也
  • Organizer: 第3回エピジェネティクス研究会
  • Place of Presentation: 学術総合センター、東京
• [Presentation] ヒト脳神経系細胞におけるDNAメチル化解析-全ゲノムメチル化量の定量とメチル化領域の個人間差異の検討- (2009)
  • Author(s): 岩本和也、文東美紀、上田順子、中野洋子、鵜飼渉、橋本恵理、齋藤利和、加藤忠史
  • Organizer: 第31回日本生物学的精神医学会
  • Place of Presentation: 京都
• [Presentation] 双極性障害患者死後脳を用いた遺伝子発現解析 (2008)
  • Author(s): 岩本和也、文東美紀、加藤忠史
  • Organizer: 第27回躁うつ病の薬理・生化学的研究懇話会
  • Place of Presentation: 箱根
  • Year and Date: 2008-06-21
• [Presentation] Exploring the effect of genetic variations on gene expression in human brain and its application to genetics of bipolar disorder. Symposium on Genome-wide studies of bipolar disorder and mood stabilization. (2008)
  • Author(s): Iwamoto K, Kato T.
  • Organizer: The 16th World Congress of Psychiatric Genetics
  • Place of Presentation: Osaka
• [Presentation] DNA methylation analysis in the human brain : promoter tiling array analysis of neuronal and non-neuronal nuclei. (2008)
  • Author(s): Iwamoto K, Bundo M, Ueda J, Nakano Y, Ukai W, Hashimoto E, Saito T, Kato T
  • Organizer: The 16th World Congress of Psychiatric Genetics
  • Place of Presentation: Osaka
• [Presentation] Epigenetic Analysis in Neuronal and Non-neuronal Cells and its Application for Psychiatric Disease. (2008)
  • Author(s): Iwamoto K, Bundo M, Ueda J, Nakano Y, Ukai W, Hashimoto E, Saito T, Kato T.
  • Organizer: 2nd WFSBP Asia-Pacific Congress and 39th Annual Meeting of JSBP.
  • Place of Presentation: Toyama
• [Presentation] DNA methylation analysis of neuronal and non-neuronal nuclei in the human prefrontal cortex : promoter tiling array analysis (2008)
  • Author(s): 岩本和也、文東美紀、上田順子、中野陽子、鵜飼渡、橋本恵理、齋藤利和、加藤忠史
  • Organizer: 第31回日本分子生物学会・第81回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド、兵庫
• [Presentation] ヒト脳遺伝子発現における遺伝的多型の影響評価と精神疾患研究への応用 (2008)
  • Author(s): 岩本和也、加藤忠史
  • Organizer: 第53回人類遺伝学会
  • Place of Presentation: パシフィコ横浜、神奈川
• [Presentation] DNA methylation analysis in human neuronal and non-neuronal cells (2008)
  • Author(s): 岩本和也、文東美紀、上田順子、中野陽子、鵜飼渡、橋本恵理、斉藤利和、加藤忠史
  • Organizer: 第2回エピジェネティクス研究会年会
  • Place of Presentation: 東レ総合研修センター、三島
• [Presentation] DNA methylation analysis of neuronal and non-neuronal nuclei in the human prefrontal cortex : promoter tiling array analysis (2008)
  • Author(s): 岩本和也
  • Organizer: 第31回日本分子生物学会・第81回日本生化学会合同大会
  • Place of Presentation: 神戸
• [Presentation] ヒト脳遺伝子発現における遺伝的多型の影響評価と精神疾患研究への応用 (2008)
  • Author(s): 岩本和也
  • Organizer: 第53回人類遺伝学会
  • Place of Presentation: 横浜
• [Presentation] DNA methylation analysis in human neuronal and non-neuronal cells (2008)
  • Author(s): 岩本和也
  • Organizer: 第2回エピジェネティクス研究会年会
  • Place of Presentation: 三島
• [Presentation] Exploring the effect of genetic variations on gene expression in human brain and its application to genetics of bipolar disorder. (2008)
  • Author(s): Kazuya Iwamoto
  • Organizer: The 16th World Congress of Psychiatric Genetics
  • Place of Presentation: Osaka
• [Presentation] DNA methylation analysis in the human brain : promoter tiling array analysis of neuronal and non-neuronal nuclei (2008)
  • Author(s): Kazuya Iwamoto
  • Organizer: The 16th World Congress of Psychiatric Genetics
  • Place of Presentation: Osaka
• [Presentation] Epigenetic analysis in neuronal and non-neuronal cells and its application for psychiatric disease (2008)
  • Author(s): Kazuya Iwamoto
  • Organizer: 2^<nd> WFSBP Asia-Pacific Congress and 39^<th> Annual Meeting of JSBP
  • Place of Presentation: Toyama
• [Presentation] Comprehensive DNA methylation analysis in the human brain and its application to psychiatric diseases.
  • Author(s): Iwamoto K, Bundo M, Ueda J, Nakano Y, Oldham MC, Ukai W, Hashimoto E, Saito T, Geschwind DH, Kato T.
  • Organizer: The 17th World Congress of Psychiatric Genetics
  • Place of Presentation: Manchester Grand Hyatt, SanDiego
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