Development of gene therapy for vascular disease using multi-functional polymeric micellar gene vector

• Project/Area Number: 20689024
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Nagasaki University (2010-2011); The University of Tokyo (2008-2009)
• Principal Investigator: OBA Makoto 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (20396716)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000); Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 血管外科学 / 遺伝子治療 / ドラッグデリバリーシステム / 非ウイルス遺伝子ベクター / ドラッグテリバリーシステム

Research Abstract

I developed systemic gene delivery systems for vascular diseases using polymeric micelles, composed of poly(ethylene glycol)-polycation block copolymers and plasmid DNA. I designed polymeric micelles with various intelligent functions, and they achieved an enhancement of stability in blood stream, targeting to disease regions, controlling intracellular trafficking, and effective release of encapsulated gene.

Research Products

• [Journal Article] Polyplex micelles prepared fromω-cholesteryl PEG-polycation block copolymers for systemic gene delivery (2011)
  • Author(s): M. Oba, K. Miyata, K. Osada, R. J. Christie, M. Sanjoh, W. Li, S. Fukushima, T. Ishii, M. R. Kano, N. Nishiyama, H. Koyama, K. Kataoka
  • Journal Title: Biomaterials Volume: 32(2) Pages: 652-663
  • Peer Reviewed
• [Journal Article] Antiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked polyplex micelles (2011)
  • Author(s): Y. Vachutinsky, M. Oba, K. Miyata, S. Hiki, M. R. Kano, N. Nishiyama, H. Koyama, K. Miyazono, K. Kataoka
  • Journal Title: J. Control. Release Volume: 149(1) Pages: 51-57
  • Peer Reviewed
• [Journal Article] Effect of integrin targeting and PEG shielding on polyplex micelle internalization studied by live-cell imaging (2011)
  • Author(s): F. M. Konig, Y. Vachutinsky, M. Oba, K. Miyata, N. Nishiyama, K. Kataoka, C. Brauchle, N. Ruthardt
  • Journal Title: J. Control. Release Volume: 156(3) Pages: 364-373
  • Peer Reviewed
• [Journal Article] Polylex micelles prepared from ω-cholesteryl PEG-polycation block copolymers for systemic gene delivery (2011)
  • Author(s): M.Oba, K.Miyata, K.Osada, R.J.Christie, M.Sanjoh, W.Li, S.Fukushima, T.Ishii, M.R.Kano, N.Nishiyama, H.Koyama, K.Kataoka
  • Journal Title: Biomaterials Volume: 32(2) Issue: 2 Pages: 652-663
  • DOI: 10.1016/j.biomaterials.2010.09.022
  • Peer Reviewed
• [Journal Article] Antaiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked polyplex micelles (2011)
  • Author(s): Y.Vachutinsky, M.Oba, K.Miyata, S.Hiki, M.R.Kano, N.Nishiyama, H.Koyama, K.Miyazono, K.Kataoka
  • Journal Title: Journal of Controlled Release Volume: 149(1) Pages: 51-57
  • Peer Reviewed
• [Journal Article] Effect of integrin targeting and PEG shielding on polyplex micelle internalization studied by live-cell imaging (2011)
  • Author(s): F.M.Konig, Y.Vachutinsky, M.Oba, K.Miyata, N.Nishiyama, K.Kataoka, C.Brauchle, N.Ruthardt
  • Journal Title: Journal of Controlled Release Volume: 151(2) Issue: 3 Pages: 104-109
  • DOI: 10.1016/j.jconrel.2011.08.003
  • Peer Reviewed
• [Journal Article] Polyion complex stability and gene silencing efficiency with a siRNA-grafted polymer delivery system (2010)
  • Author(s): H. Takemoto, A. Ishii, K. Miyata, M. Nakanishi, M. Oba, T. Ishii, Y. Yamasaki, N. Nishiyama, K. Kataoka
  • Journal Title: Biomaterials Volume: 31(31) Pages: 8097-8105
  • Peer Reviewed
• [Journal Article] Introduction of stearoyl moieties into a biocompatible cationic polyaspartamide derivative, PAsp(DET), with endosomal escaping function for enhanced siRNA-mediated gene knockdown (2010)
  • Author(s): H. J. Kim, A. Ishii, K. Miyata, Y. Lee, S. Wu, M. Oba, N. Nishiyama, K. Kataoka
  • Journal Title: J. Control. Release Volume: 145(2) Pages: 141-148
  • Peer Reviewed
• [Journal Article] pDNA/ poly(L-lysine) polyplexes functionalized with a pH-sensitive charge-conversional poly(aspartamide) derivative for controlled gene delivery to human umbilical vein endothelial cells (2010)
  • Author(s): M. Sanjoh, S. Hiki, Y. Lee, M. Oba, K. Miyata, T. Ishii, K. Kataoka
  • Journal Title: Macromol. Rapid Comm Volume: 31(13) Pages: 1181-1186
  • Peer Reviewed
• [Journal Article] Antiangiogenic gene therapy of solid tumor by systemic injection of polyplex micelles loading plasmid DNA encoding soluble Flt-1 (2010)
  • Author(s): M. Oba, Y. Vachutinsky, K. Miyata, M. R. Kano, S. Ikeda, N. Nishiyama, K. Itaka, K. Miyazono, H. Koyama, K. Kataoka
  • Journal Title: Mol. Pharmaceutics Volume: 7(2) Pages: 501-509
  • Peer Reviewed
• [Journal Article] Enhanced transfection with silica-coated polyplexes loading plasmid DNA (2010)
  • Author(s): K. Miyata, N. Gouda, H. Takemoto, M. Oba, Y. Lee, H. Koyama, Y. Yamasaki, K. Itaka, N. Nishiyama, K. Kataoka
  • Journal Title: Biomaterials Volume: 31(17) Pages: 4764-4770
  • Peer Reviewed
• [Journal Article] Enhanced transfection with silica-coated polyplexes loading plasmid DNA (2010)
  • Author(s): Kanjiro Miyata, Noha Gouda, Hiroyasu Takemoto, Makoto Oba, Yan Lee, Hiroyuki Koyama, Yuichi Yamasaki, Keiji Itaka, Nobuhiro Nishiyama, Kazunori Kataoka
  • Journal Title: Biomaterials 31(17) Pages: 4764-4770
  • Peer Reviewed
• [Journal Article] Antiangiogenic gene therapy of solid tumor by systemic injection of polyplex micelles loading plasmid DNA encoding soluble Flt-1 (2010)
  • Author(s): Makoto Oba, Yelena Vachutinsky, Kanjiro Miyata, Mitsunobu R.Kano, Sorato Ikeda, Nobuhiro Nishiyama, Keiji Itaka, Kohei Miyazono, Hiroyuki Koyama, Kazunori Kataoka
  • Journal Title: Mol.Pharmaceutics 7(2) Pages: 501-509
  • Peer Reviewed
• [Journal Article] Antiangiogenic gene therapy of solid tumor by systemic injection of polyplex micelles loading plasmid DNA encoding soluble Flt-1 (2010)
  • Author(s): M.Oba, Y.Vachutinsky, K.Miyata, M.R.Kano, S.Ikeda, N.Nishiyama, K.Itaka, K.Miyazono, H.Koyama, K.Kataoka
  • Journal Title: Molecular Pharmaceutics Volume: 7(2) Pages: 501-509
  • Peer Reviewed
• [Journal Article] pDNA/poly(L-lysine) polyplexes functionalized with a pH-sensitive charge-conversional poly(aspartamide) derivative for controlled gene delivery to human umbilical vein endothelial cells (2010)
  • Author(s): M.Sanjoh, S.Hiki, Y.Lee, M.Oba, K.Miyata, T.Ishii, K.Kataoka
  • Journal Title: Macromolecular Rapid Communications Volume: 31(13) Pages: 1181-1186
  • Peer Reviewed
• [Journal Article] Introduction of stearoyl moieties into a biocompatible cationic polyaspartamide derivative, PAsp(DET), with endosomal escaping function for enhanced siRNA-mediated gene knockdown (2010)
  • Author(s): H.J.Kim, A.Ishii, K.Miyata, Y.Lee, S.Wu, M.Oba, N.Nishiyama, K.Kataoka
  • Journal Title: Journal of Controlled Release Volume: 145(2) Pages: 141-148
  • Peer Reviewed
• [Journal Article] Antiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked micelles (2010)
  • Author(s): Y.Vachutinsky, M.Oba, et al
  • Journal Title: Journal of Controlled Release (in press)
  • Peer Reviewed
• [Journal Article] Anti-angiogenic gene therapy of solid tumor by systemic injection Of polyplex micelles loading plasmid DNA encoding soluble Flt-1 (2010)
  • Author(s): M.Oba, et al
  • Journal Title: Molecular Pharmaceutics 7(2) Pages: 501-509
  • Peer Reviewed
• [Journal Article] siRNA-based therapy of glomerulonephritis (2010)
  • Author(s): H.Shimizu, Y.Hori, S.Kaname, K.Yamada, N.Nishiyama, S.Matsumoto, K.Miyata, M.Oba, et al
  • Journal Title: Journal of the American Society of Nephrology 21 Pages: 622-633
  • Peer Reviewed
• [Journal Article] Enhanced percolation and gene expression in tumor hypoxia by PEGylated polyplex micelles (2009)
  • Author(s): M. Han, M. Oba, N. Nishiyama, M. R. Kano, S. Kizaka-Kondoh, K. Kataoka
  • Journal Title: Mol. Ther Volume: 17(8) Pages: 1404-1410
  • Peer Reviewed
• [Journal Article] Environmental-responsive block copolymer micelles with a disulfide cross-linked core for enhanced siRNA delivery (2009)
  • Author(s): S. Matsumoto, R. J. Christie, N. Nishiyama, K. Miyata, A. Ishii, M. Oba, H. Koyama, Y. Yamasaki, K. Kataoka
  • Journal Title: Biomacromolecules Volume: 10(1) Pages: 119-127
  • Peer Reviewed
• [Journal Article] Enhanced percolation and gene expression in tumor hypoxia by PEGylated polyplex micelles (2009)
  • Author(s): Muri Han, Makoto Oba, Nobuhiro Nishiyama, Mitsunobu R.Kano, Shinae Kizaka-Kondoh, Kazunori Kataoka
  • Journal Title: Mol.Ther 17(8) Pages: 1404-1410
  • Peer Reviewed
• [Journal Article] Enhanced percolation and gene expression in tumor hypoxia by PEGylated polyplex micelles (2009)
  • Author(s): M.Han, M.Oba, et al
  • Journal Title: Molecular Therapy 17(8) Pages: 1404-1410
  • Peer Reviewed
• [Journal Article] Environmental-responsive block copolymer micelles with a disulfide cross-linked core for enhanced siRNA delivery (2009)
  • Author(s): S.Matsumoto, R.J.Christie, N.Nishiyama, K.Miyata, A.Ishii, M.Oba, et al
  • Journal Title: Biomacromolecules 10(1) Pages: 119-127
  • Peer Reviewed
• [Journal Article] Polyplexes from poly(aspartamide) bearing 1, 2-diaminoethane side chains induce pH-selective, endosomal membrane destabilization with amplified transfection and negligible cytotoxicity (2008)
  • Author(s): K. Miyata, M. Oba, M. Nakanishi, S. Fukushima, Y. Yamasaki, H. Koyama, N. Nishiyama, K. Kataoka
  • Journal Title: J. Am. Chem. Soc Volume: 130(8) Pages: 16287-16294
  • Peer Reviewed
• [Journal Article] Polyplex micelles from triblock copolymers composed of tandemly aligned segments with biocompatible, endosomal escaping, and DNA-condensing functions for systemic gene delivery to pancreatic tumor tissue (2008)
  • Author(s): K. Miyata, M. Oba, M. R. Kano, S. Fukushima, Y. Vachutinsky, M. Han, H. Koyama, K. Miyazono, N. Nishiyama, K. Kataoka
  • Journal Title: Pharm. Res Volume: 25(12) Pages: 2924-2936
  • Peer Reviewed
• [Journal Article] Polyplex micelles with cyclic RGD peptide ligands and disulfide crosslinks directing to the enhanced transfection via controlled intracellular trafficking (2008)
  • Author(s): M. Oba, K. Aoyagi, K. Miyata, Y. Matsumoto, K. Itaka, N. Nishiyama, Y. Yamasaki, H. Koyama, K. Kataoka
  • Journal Title: Mol. Pharmaceutics Volume: 5(6) Pages: 1080-1092
  • Peer Reviewed
• [Journal Article] Polyplex micelles with cyclic RGD peptide ligands and disulfide crosslinks directing to the enhanced transfetion via controlled intracellular trafficking (2008)
  • Author(s): Makoto Oba, Kazuhiro Aoyagai, Kanjiro Miyata, Yu Matsumoto, Keiji Itaka, Nobuhiro Nishiyama, Yuichi Yamasaki, Hiroyuki Koyama, Kazunori Kataoka
  • Journal Title: Mol.Pharmaceutics 5(6) Pages: 1080-1092
  • Peer Reviewed
• [Journal Article] Polyplexes from poly(aspartamide) bearing 1, 2-diaminoethane side chains induce pH-selective, endosomal membrane destabilization with amplified transfection and negligible cytotoxicity (2008)
  • Author(s): Kanjiro Miyata, Makoto Oba, Masataka Nakanishi, Shigeto Fukushima, Yuichi Yamasaki, Hiroyuki Koyama, Nobuhiro Nishiyama, Kazunori Kataoka
  • Journal Title: J.Am.Chem.Soc 130(48) Pages: 16287-16294
  • Peer Reviewed
• [Journal Article] Polyplex micelles with cyclic RGD peptide ligands and disulfide crosslinks directing to the enhanced transfection via controlled intracellular trafficking (2008)
  • Author(s): M. Oba, et.al.
  • Journal Title: Molecular Pharmaceutics 5(6) Pages: 1080-1092
  • Peer Reviewed
• [Journal Article] Polyplex micelles from triblock copolymers composed of tandemly aligned segments with biocompatible, endosomal escaping, and DNA-condensing functions for systemic gene delivery to pancreatic tumor tissue (2008)
  • Author(s): K. Miyata, M. Oba, et.al.
  • Journal Title: Pharmaceutical Research 25(12) Pages: 2924-2936
  • Peer Reviewed
• [Journal Article] Polyplexes from poly(aspartamide)bearing 1,2-diaminoethane side chains induce pH-sensitive, endosomal membrane destabilization with amplified transfection and neglible cytotoxicity (2008)
  • Author(s): K. Miyata, M. Oba, et.al.
  • Journal Title: Journal of the American Chemical Society 130(48) Pages: 16287-16294
  • Peer Reviewed
• [Presentation] PEG-detachable polyaspartamide derivative block copolymer bearing stearoyl moieties for in vivo siRNA delivery (2011)
  • Author(s): H. J. Kim, M. Oba, F. Pittella, T. Nomoto, H. Cabral, Y. Matsumoto, K. Miyata, N. Nishiyama, K. Kataoka
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 岡山
• [Presentation] DDS素材としての可能性を秘めた非天然型アミノ酸含有ペプチドに関する基礎的研究 (2011)
  • Author(s): 大庭誠, 平田陽子, 加藤和也, 田中正一
  • Organizer: 第27回日本DDS学会学術集会
  • Place of Presentation: 東京
• [Presentation] Enhanced transfection by binary polyion complex from block-and homo-polycations towards systemic gene therapy (2011)
  • Author(s): Q. Chen, M. Oba, T. Ishii, K. Osada, K. Kataoka
  • Organizer: 第60回高分子年次大会
  • Place of Presentation: 大阪
• [Presentation] Enhanced transfection by binary polyion complex from block- and homo-polycations towards systemic gene therapy (2011)
  • Author(s): Qixian Chen, Makoto Oba, Takehiko Ishii, Kensuke Osada, Kazunori Kataoka
  • Organizer: 第60回高分子年次大会
  • Place of Presentation: 大阪
• [Presentation] Antiangiogenic gene therapy of subcutaneous pancreatic tumor by systemic injection of polyplex micelles loading sFlt-1 plasmid DNA (2010)
  • Author(s): M. Oba, Y. Vachutinsky, K. Miyata, N. Nishiyama, H. Koyama, K. Kataoka
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: Honolulu, Hawaii, USA
• [Presentation] 化学量論的な電荷比を超えて会合する高分子ミセル型遺伝子ベクターの設計とその機能評価 (2010)
  • Author(s): 大庭誠, 宮田完二郎, 長田健介, 福島重人, 西山伸宏, 小山博之, 片岡一則
  • Organizer: 遺伝子・デリバリー研究会第10回シンポジウム
  • Place of Presentation: 札幌
• [Presentation] Antiangiogenic gene therapy of subcutaneous pancreatic tumor by systemic injection of polyplex micelles loading sFlt-1 plasmid DNA (2010)
  • Author(s): Makoto Oba, Yelena Vachutinsky, Kanjiro Miyata, Nobuhiro Nishiyama, Hiroyuki Koyama, Kazunori Kataoka
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: Honolulu
• [Presentation] 化学両論的な電化比を超えて会合する高分子ミセル型遺伝子ベクターの設計とその機能評価 (2010)
  • Author(s): 大庭誠, 宮田完二郎, 長田健介, 福島重人, 西山伸宏, 小山博之, 片岡一則
  • Organizer: 遺伝子・デリバリー研究会第10回シンポジウム
  • Place of Presentation: 札幌
• [Presentation] Antiangiogenic gene therapy of subcutaneous pancreatic tumor by systemic injection of polyplex micelles loading sFlt-1 plasmid DNA (2010)
  • Author(s): M.Oba, Y.Vachutinsky, K.Miyata, N.Nishiyama, H.Koyama, K.Kataoka
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: Honolulu, USA
• [Presentation] Anti-angiogenic gene therapy of pancreatic adenocarcinoma using polyplex micelles with plasmid DNA encoding soluble form of Flt-1 (2009)
  • Author(s): M. Oba, Y. Vachutinsky, K. Miyata, S. Ikeda, N. Nishiyama, H. Koyama, K. Kataoka
  • Organizer: The 10th US-Japan Symposium on Drug Delivery System
  • Place of Presentation: Maui
• [Presentation] 高分子ミセル型遺伝子ベクターを用いた膵臓がんの遺伝子治療法の開発 (2009)
  • Author(s): 大庭誠, Y. Vachutinsky, 宮田完二郎, 狩野光伸, 宮園浩平, 西山伸宏, 小山博之, 片岡一則
  • Organizer: 第31回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
• [Presentation] 膵臓がんの遺伝子治療を目指した高分子ミセル型遺伝子ベクターの開発 (2009)
  • Author(s): 大庭誠, Y. Vachutinsky, 宮田完二郎, 狩野光伸, 宮園浩平, 西山伸宏, 位高啓史, 小山博之, 片岡一則
  • Organizer: 遺伝子・デリバリー研究会第9回シンポジウム
  • Place of Presentation: 大阪
• [Presentation] 高分子ミセル型遺伝子ベクターを用いた膵臓がんの遺伝子治療法の開発 (2009)
  • Author(s): 大庭誠, Vachutinsky Yelena, 宮田完二郎, 狩野光伸, 宮園浩平, 西山伸宏, 小山博之, 片岡一則
  • Organizer: 第31回日本バイオマテリアル学会
  • Place of Presentation: 京都
• [Presentation] Anti-angiogenic gene therapy of pancreatic adenocarcinoma using ppolyplex micelles with plasmid DNA encoding soluble form of Flt-1 (2009)
  • Author(s): Makoto Oba, Yelena Vachutinsky, Kanjiro Miyata, Sorato Ikeda, Nobuhiro Nishiyama, Hiroyuki Koyama, Kazunori Kataoka
  • Organizer: The 10th US-Japan Symposium on Drug Delivery System
  • Place of Presentation: Maui
• [Presentation] Anti-angiogenic gene therapy of pancreatic adenocarcinoma using polyplex micelles with plasmid DNA encoding soluble form of Flt-1 (2009)
  • Author(s): 大庭誠, et al
  • Organizer: The 10^<th> US-Japan Symposium on Drug Delivery System
  • Place of Presentation: Maui, USA
• [Presentation] Anti-angiogenic gene therapy for pancreatic cancer by systemic administration of polyplex micelles (2008)
  • Author(s): M. Oba
  • Organizer: CMSI-GCOE Students Workshop : Frontiers in Nanomedicine
  • Place of Presentation: Tokyo
  • Year and Date: 2008-11-19
• [Presentation] 疎水性相互作用により安定化されたポリプレックスミセル型遺伝子ベクター (2008)
  • Author(s): 大庭誠, 宮田完二郎, 福島重人, 長田健介, 位高啓史, 西山伸宏, 小山博之, 片岡一則
  • Organizer: 遺伝子・デリバリー研究会第8回シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2008-05-09
• [Presentation] 疎水性相互作用を利用した高分子ミセル型遺伝子キャリアの開発 (2008)
  • Author(s): 大庭誠, 宮田完二郎, 福島重人, 位高啓史, 西山伸宏, 小山博之, 片岡一則
  • Organizer: 第37回医用高分子シンポジウム
  • Place of Presentation: 東京
• [Presentation] Functional assessment of polyplex micelles with cyclic RGD peptide ligands and disulfide crosslinked core (2008)
  • Author(s): M. Oba, K. Aoyagi, K. Miyata, N. Nishiyama, H. Koyama, K. Kataoka
  • Organizer: 35^<th> Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: New York
• [Presentation] 疎水性相互作用を利用した高分子ミセル型遺伝子ベクターの開発 (2008)
  • Author(s): 大庭誠, 宮田完二郎, 福島重人, 長田健介, 位高啓史, 西山伸宏, 小山博之, 片岡一則
  • Organizer: 第24回日本DDS学会
  • Place of Presentation: 東京
• [Presentation] Polyplex micelles from plasmid DNA and PEG-based block copolymer with environmental-responsive crosslinks and targetable ligands (2008)
  • Author(s): M. Oba, K. Aoyagi, K. Miyata, K. Itaka, N. Nishiyama, H. Koyama, K. Kataoka
  • Organizer: 第14回日本遺伝子治療学会総会
  • Place of Presentation: Sapporo
• [Presentation] Functional assessment of polyplex micelles with cyclic RGD peptide ligands and disulfide crosslinked core (2008)
  • Author(s): Makoto Oba, Kazuhiro Aoyagai, Kanjiro Miyata, Nobuhiro Nishiyama, Hiroyuki Koyama, Kazunori Kataoka
  • Organizer: 35th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: New York
• [Presentation] Functional assessment of polyplex micelles with cyclic RGD peptide ligands and disulfide crosslinked core (2008)
  • Author(s): M. Oba, et al
  • Organizer: 35^<th> Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: New York, USA
• [Remarks]
  • URL: http://www.bmw.t.u-tokyo.ac.jp/
• [Patent(Industrial Property Rights)] 電荷変換型三元系ポリプレックス (2009)
  • Inventor(s): 片岡一則, 宮田完二郎, Lee Yan, 大庭誠, 西山伸宏, 比木茂寛, 三條 舞, Kim Hyn Jin
  • Industrial Property Rights Holder: 片岡一則, 宮田完二郎, Lee Yan, 大庭誠, 西山伸宏, 比木茂寛, 三條 舞, Kim Hyn Jin
  • Filing Date: 2009-04-30
• [Patent(Industrial Property Rights)] エンドソーム脱出機能を有する電荷変換型二元系ポリプレックス (2008)
  • Inventor(s): 片岡一則、YanLee、宮田完二郎、大庭誠
  • Industrial Property Rights Holder: 東京大学
  • Filing Date: 2008-04-30
  • Overseas