Elucidation for the GABA(A) receptor-medicated inhibitory effect onbronchospasm
| Project/Area Number |
20689036
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
MIZUTA Kentaro Tohoku University, 大学院・歯学研究科, 助教 (40455796)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2008: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
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| Keywords | 気管支痙攣 / GABA(A)受容体 / 全身麻酔薬 / カルシウムイオン / Ca^<2+> / GABA (A)受容体 / GABA / 受容体 / IP_3 |
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| Research Abstract |
The transient intracellular Ca^<2+> ([Ca^<2+>]_i) increase in the human airway smooth muscle cells (HASM) during the contraction of the airway smooth muscle was attenuated by GABAA receptor agonist. Following (1) withdrawal of extracellular Ca^<2+>, or (2) blockade the voltage-gated Ca^<2+> channel blocker, GABAA receptor agonist did not attenuate the transient [Ca^<2+>]_i increases in HASM In contrast, (3) following depletion of Ca^<2+> in the sarcoplasmic reticulum, GABAA receptor agonist retained its ability to attenuate the transient [Ca^<2+>]_i increase. GABA also induced the membrane hyperpolarization on HASM.
These results suggest that the activation of the GABAA receptors on airway smooth muscle cells attenuated extracellular Ca^<2+> influx through the membrane hyperpolarization and facilitates airway smooth muscle relaxation.
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Report
(3 results)
Research Products
(34 results)
