Molecular mechanisms of establishment of brain asymmetry

• Project/Area Number: 20700281
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Tohoku University
• Principal Investigator: MASANORI Takahashi Tohoku University, 大学院・医学系研究科, 助教 (20361074)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 分子・細胞神経科学 / 左右非対称性 / カドヘリン / 大脳皮質 / 線条体 / 活動依存性

Research Abstract

In this study, I examined characters of cells expressing cadherin20 in the lateral striatal region and somatosensory cortex in adult rats using in situ hybridization and immunohistochemistry. These studies revealed that all cadherin20+ cells in the lateral striatum were both direct and indirect pathways medium spiny projection neurons. I also established the method to determine relationship between asymmetric expression of cadherin20 and handedness in rats. We could distinguish left or right-handedness of rats using this method.

Research Products

• [Journal Article] Dynamic expression of Six family genes in the dental mesenchyme and the epithelial ameloblast stem/progenitor cells during murine tooth development. (2010)
  • Author(s): Nonomura, K., Takahashi, M*., Wakamatsu, Y. Takano-Yamamoto, T., Osumi, N.
  • Journal Title: J. Anat. 216(Corresponding author) Pages: 80-91
  • Peer Reviewed
• [Journal Article] Downstream genes of Pax6 revealed by comprehensive transcriptome profiling in the developing rat hindbrain. (2010)
  • Author(s): Numayama-Tsuruta, K., Arai, Y., Takahashi, M., Sasaki-Hoshino, M., Funatsu, N., Nakamura, S., Osumi, N.
  • Journal Title: BMC Dev. Biol. 10 Pages: 6-6
  • Peer Reviewed
• [Journal Article] Dynamic expression of Six family genes in the dental mesenchyme and the epithelial ameloblast stem/progenitor cells during murine tooth development (2010)
  • Author(s): Nonomura, K., Takahashi, M., Wakamatsu, Y., Takano-Yamamoto, T., Osumi, N.
  • Journal Title: J.Anat. 216 Pages: 80-91
  • Peer Reviewed
• [Journal Article] Downstream genes of Pax6 revealed by comprehensive transcriptome profiling in the developing rat hindbrain (2010)
  • Author(s): Numayama-Tsuruta, K., Arai, Y., Takahashi, M., Sasaki-Hoshino, M., Funatsu,N., Nakamura, S., Osumi, N.
  • Journal Title: BMC Dev.Biol. 10 Pages: 6-6
  • Peer Reviewed
• [Journal Article] Expression study of cadherin7 and cadherin20 in the embryonic and adult rat central nervous system. (2008)
  • Author(s): Takahashi, M., Osumi, N.
  • Journal Title: BMC Dev. Biol. 8 Pages: 87-87
  • Peer Reviewed
• [Journal Article] Transferring genes into cultured mammalian embryos by electroporation. (2008)
  • Author(s): Takahashi, M., Nomura, T., Osumi, N.
  • Journal Title: Dev. Growth Differ. 50 Pages: 485-497
  • NAID: 10021097252
  • Peer Reviewed
• [Journal Article] Expression study of cadherin7 and cadherin20 in the embryonic and adult rat central nervous system (2008)
  • Author(s): Takahashi, M., and Osumi, N.
  • Journal Title: BMC Dev. Biol 8 Pages: 87-87
  • Peer Reviewed
• [Journal Article] Transferring genes into cultured mammalian embryos by electroporation (2008)
  • Author(s): Takahashi, M., Nomura, T., and Osumi, N.
  • Journal Title: Dev. Growth Differ. 50 Pages: 485-497
  • NAID: 10021097252
  • Peer Reviewed
• [Presentation] 哺乳類菱脳における境界の維持のメカニズムシンポジウム (2010)
  • Author(s): 高橋将文
  • Organizer: 仙台動物発生コロキウム
  • Place of Presentation: 仙台(招待講演)
  • Year and Date: 2010-01-22
• [Presentation] 哺乳類菱脳における境界の維持のメカニズム (2010)
  • Author(s): 高橋将文
  • Organizer: シンポジウム「仙台動物発生コロキウム」
  • Place of Presentation: 仙台
  • Year and Date: 2010-01-22
• [Presentation] Pax6 is involved in specification of rhombomere boundary cells in the rat hindbrain. (2009)
  • Author(s): Takahashi, M., Osumi, N.
  • Organizer: Construction and Reconstruction of the Brain.
  • Place of Presentation: Awaji
  • Year and Date: 2009-10-09
• [Presentation] Pax6 is involved in specification of rhombomere boundary cells in the rat hindbrain (2009)
  • Author(s): Takahashi, M., Osumi, N.
  • Organizer: Construction and Reconstruction of the Brain
  • Place of Presentation: Awaji
  • Year and Date: 2009-10-09
• [Presentation] Pax6 is involved in specification of rhombomereboundary cells in the rat hindbrain. (2009)
  • Author(s): Takahashi, M., Osumi, N.
  • Organizer: 第9回日本分子生物学会春期シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-11
• [Presentation] Pax6 is involved in specification of rhombomereboundary cells in the rat hindbrain. (2009)
  • Author(s): Takahashi, M., Osumi, N.
  • Organizer: CDB Symposium 2009 Shape and Polarity.
  • Place of Presentation: Kobe
  • Year and Date: 2009-03-23
• [Presentation] The expression of cad20 mRNA represents a novel subtype of striatal projection neurons and brain asymmetry. (2008)
  • Author(s): Takahashi, M., Osumi, N.
  • Organizer: 第31回日本神経科学大会Neuroscience2008
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] The expression of cad20 mRNA represents a novel subtype of striatal projection neurons and brain asymmetry (2008)
  • Author(s): Takahashi, M., and and Osumi, N
  • Organizer: 第31回日本神経科学大会Neuroscience2008
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Book] Electroporation into cultured mammalian embryos.(Electroporation and Sonoporation in Developmental Biology.) (2009)
  • Author(s): Nomura, T, Takahashi, M., Osumi, N.
  • Publisher: Springer
• [Book] Electroporation into cultured mammalian embryos. Electroporation and Sonoporation in Developmental Biology (2009)
  • Author(s): Nomura, T, Takahashi, M., and Osumi, N.
  • Total Pages: 331
  • Publisher: Springer