Functional analysis of small G protein Rac1 and Rac3 signalings during corticogenesis

• Project/Area Number: 20700289
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Kobe University
• Principal Investigator: KASSAI Hidetoshi Kobe University, 大学院・医学系研究科, 助教 (40403232)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 分子 / 細胞神経科学 / 大脳皮質 / 遺伝子改変マウス / 低分子量Gタンパク質 / Rac / マウス

Research Abstract

Rac1 and Rac3 are highly homologous proteins, and it has been quite difficult to analyze their functions separately. Here I generated the knock-in mice, in which express epitope-tagged Rac3 so that Rac1 and Rac3 are easily distinguished at the protein level. By using these knock-in mice, expression patterns of Rac1 and Rac3 are traced in the developing brains. Also, proteomic analysis of Rac3 complex was performed to clarify Rac3 signaling in the brain. I identified several proteins which specifically associate with activated Rac3.

Research Products

• [Journal Article] Rac1 in cortical projection neurons is selectively required for midline crossing of commissural axonal formation. (2008)
  • Author(s): Hidetoshi Kassai, Toshio Terashima, Masahiro Fukaya, Kazuki Nakao, Mizuho Sakahara, Masahiko Watanabe, Atsu Aiba
  • Journal Title: Eur. J. Neurosci. 28(2) Pages: 257-67
  • Peer Reviewed
• [Journal Article] Rac1 in cortical projection neurons is selectively required for midline crossing of commissural axonal formation. (2008)
  • Author(s): 葛西秀俊
  • Journal Title: European Journal of Neuroscience 28 Pages: 257-267
  • Peer Reviewed
• [Presentation] プロテオミクス手法を用いた遺伝子改変マウスの脳機能解析 (2009)
  • Author(s): 葛西秀俊
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] プロテオミクス手法を用いた遺伝子改変マウスの脳機能解析 (2009)
  • Author(s): 葛西秀俊
  • Organizer: 第32回 日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] 小脳プルキンエ細胞におけるmGluR1-Homer複合体の生理機能解析 (2009)
  • Author(s): 葛西秀俊・渡久川兼誉・橋本浩一・岡村千絵子・中尾和貴・狩野方伸・饗場篤
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] 小脳プルキンエ細胞におけるmGluR1-Homer 複合体の生理機能解析 (2009)
  • Author(s): 葛西秀俊
  • Organizer: 第32回 日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] Small G protein Rac1 is selectively required for the formation of commissural axons in the cerebral cortex (2008)
  • Author(s): Hidetoshi Kassai, Toshio Terashima, Masahiro Fukaya, Kazuki Nakao, Mizuho Sakahara, Masahiko Watanabe, Atsu Aiba
  • Organizer: 37th annual meeting of the Society for Neuroscience
  • Place of Presentation: アメリカ合衆国・ワシントンD.C.
  • Year and Date: 2008-11-16
• [Presentation] Small G protein Rac1 is selectively required for the formation of commissural axons in the cerebral cortex (2008)
  • Author(s): 葛西秀俊
  • Organizer: 37th annual meeting of the Society for Neuroscience
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-11-16
• [Presentation] 大脳皮質の交連線維形成におけるRac1の役割 (2008)
  • Author(s): 葛西秀俊・寺島俊雄・深谷昌弘・中尾和貴・坂原瑞穂・渡辺雅彦・饗場篤
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京・東京国際フォーラム
  • Year and Date: 2008-07-09
• [Presentation] 大脳皮質の交連線維形成におけるRac1の役割 (2008)
  • Author(s): 葛西秀俊
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Remarks]
  • URL: http://lar.cdbim.m.u-tokyo.ac.jp/index.html
• [Remarks]
  • URL: http://lar.cdbim.m.u-tokyo.ac.jp/index.html