Asymmetric endocytosis across the growth cone drives repulsive axon guidance

• Project/Area Number: 20700302
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TOJIMA Takuro The Institute of Physical and Chemical Research, 神経成長機構研究チーム, 研究員 (00373332)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 神経成長円錐 / 軸索ガイダンス / クラスリン依存性エンドサイトーシス / ダイナミン1 / カルシウム / カルシニューリン / 全反射蛍光顕微鏡法 / ケージド化合物光解離法 / エンドサイトーシス / クラスリン / ダイナミン

Research Abstract

The neuronal growth cone navigates the axon along the correct path by sensing extracellular attractive and repulsive guidance cues. In this project, we investigated a driving mechanism of growth cone repulsion. We showed that the activity of clathrin-mediated endocytosis becomes polarized in growth cones and that such asymmetry in endocytosis is required and sufficient for repulsive growth cone guidance.

Research Products

• [Journal Article] Asymmetric clathrin- mediated endocytosis drives repulsive growth cone guidance. (2010)
  • Author(s): Takuro Tojima, Rurika Itofusa, Hiroyuki Kamiguchi
  • Journal Title: Neuron 66 Pages: 370-377
  • Peer Reviewed
• [Journal Article] The nitric oxide-cGMP pathway controls the directional polarity of growth cone guidance via modulating cytosolic Ca2+ signals. (2009)
  • Author(s): Takuro Tojima, Rurika Itofusa, Hiroyuki Kamiguchi
  • Journal Title: Journal of Neuroscience 29 Pages: 7886-7897
  • Peer Reviewed
• [Journal Article] DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death. (2009)
  • Author(s): Kenji Amano, Morimitsu Fujii, Satoru Arata, Takuro Tojima, Masaharu Ogawa, Noriyuki Morita, Atsushi Shimohata, Teiichi Furuichi, Shigeyoshi Itohara, Hiroyuki Kamiguchi, Julie Korenberg, Akiko Arata, Kazuhiro Yamakawa
  • Journal Title: Journal of Neuroscience 29 Pages: 2984-2996
  • Peer Reviewed
• [Journal Article] The nitric oxide-cGMP pathway controls the directional polarity of growth cone guidance via modulating cytosolic Ca^<2+> signals (2009)
  • Author(s): Takuro Tojima
  • Journal Title: The Journal of Neuroscience 29 Pages: 7886-7897
  • Peer Reviewed
• [Journal Article] DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death (2009)
  • Author(s): Kenji Amano
  • Journal Title: The Journal of Neuroscience (印刷中)
  • Peer Reviewed
• [Journal Article] 誘引性・反発性軸索ガイダンスを制御するカルシウムシグナル (2008)
  • Author(s): 戸島拓郎, 上口裕之
  • Journal Title: 実験医学 26 Pages: 1852-1858
• [Journal Article] カルシウムシグナルによる軸索ガイダンスの制御機構 (2008)
  • Author(s): 秋山博紀, 戸島拓郎, 大芦典子, 上口裕之
  • Journal Title: 蛋白質核酸酵素 53 Pages: 153-163
• [Journal Article] 誘引性・反発性軸索ガイダンスを制御するカルシウムシグナル (2008)
  • Author(s): 戸島拓郎
  • Journal Title: 実験医学 26 Pages: 1852-1858
• [Presentation] 神経成長円錐ガイダンスにおけるクラスリン依存性エンドサイトーシスの役割 (2010)
  • Author(s): 戸島拓郎, 糸総るり香, 上口裕之
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-19
• [Presentation] Asymmetric endocytosis drives growth cone repulsion downstream of Ca2+ signals. (2009)
  • Author(s): Takuro Tojima, Rurika Itofusa, Hiroyuki Kamiguchi
  • Organizer: 39th Annual Meeting Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-10-18
• [Presentation] The role of nitric oxide-cyclic GMP pathway in switching the directional polarity of growth cone guidance. (2009)
  • Author(s): Rurika Itofusa, Takuro Tojima, Hiroyuki Kamiguchi
  • Organizer: 39th Annual Meeting Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-10-18
• [Presentation] Asymmetric endocytosis drives growth cone repulsion downstream of Ca^<2+> signals (2009)
  • Author(s): Takuro Tojima
  • Organizer: 39th Annual Meeting Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-10-18
• [Presentation] The role of nitric oxide-cyclic GMP pathway in switching the directional polarity of growth cone guidance (2009)
  • Author(s): Rurika Itofusa
  • Organizer: 39th Annual Meeting Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-10-18
• [Presentation] 反発性成長円錐ガイダンスにおける非対称性エンドサイトーシスの関与 (2009)
  • Author(s): 戸島拓郎, 糸総るり香, 上口裕之
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] 成長円錐局所エンドサイトーシスによる反発性軸索ガイダンス制御 (2009)
  • Author(s): 戸島拓郎, 糸総るり香, 上口裕之
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-03
• [Presentation] 神経軸索ガイダンスを駆動する成長円錐での非対称性膜トラフィッキング (2008)
  • Author(s): 戸島拓郎, 秋山博紀, 糸総るり香, 上口裕之
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] 一酸化窒素による反発性軸索ガイダンスの制御 (2008)
  • Author(s): 糸総るり香, 戸島拓郎, 上口裕之
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] カルシウムシグナルによる神経突起ガイダンスの制御機構 (2008)
  • Author(s): 戸島拓郎, 秋山博紀, 糸総るり香, 上口裕之
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-29
• [Book] 脳の発生と発達(岡本仁(編)) (2008)
  • Author(s): 戸島拓郎, 上口裕之
  • Publisher: 東京大学出版会
• [Remarks]
  • URL: http://www.riken.jp/r-world/research/lab/nokagaku/recovery/neuronal/index.html
• [Remarks]
  • URL: http://www.brain.riken.jp/jp/h_kamiguchi.html
• [Remarks]
  • URL: http://www.brain.riken.jp/labs/kamiguchi/