Analysis of cortico-basal ganglia loop circuits with novel retrograde transneuronal tracing technique
| Project/Area Number |
20700308
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
INOUE Ken-ichi Tokyo Metropolitan Organization for Medical Research, 霊長類研究所, 特定助教 (90455395)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 神経科学 / 脳-神経 / 脳情報処理 / 解剖学 / 大脳基底核 / ウイルスベクター / 脳・神経 / 解部学 / バイオラクノロジー / ウィルス / バイオテクノロジー / ウイルス |
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| Research Abstract |
By means of retrograde transneuronal labeling technique with rabies virus, the structural basis for cortico-basal ganglia loop circuits was analyzed in macaque monkeys. We demonstrated the existence of a closed, parallel loop involving the dorsal striatum and an open loop involving the ventral striatum. Moreover, we performed cloning of full-length genome and all genes of the CVS strain of rabies virus, and established the rescue system for a rabies virus vector from cDNA plasmid. This enables us to achieve multi-color retrograde transneuronal tracing with maker proteins such as GFP and RFP.
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Report
(3 results)
Research Products
(34 results)
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[Presentation] Efficient retrograde gene transfer into primate brain with an HIV-1-based lentiviral vector pseudotyped with rabies virus glycoprotein2008
Author(s)
Inoue K, Kato S, Kobayashi K, Yasoshima Y, Miyachi S, Inoue S, Hanawa H, Shimada T, Kobayashi K, Takada M
Organizer
38th Annual Meeting of Society for Neuroscience
Place of Presentation
Washington, D. C
Year and Date
2008-11-16
Related Report
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[Presentation] Efficient gene transfer system through retrograde axonal transport with a human immunodeficiency virus type 1-based vector pseudotyped with rabies virus glycoprotein.2008
Author(s)
Kato S, Inoue K, Kobayashi K, Yasoshima Y, Miyachi S, Inoue S, Hanawa H, Shimada T, Takada M, Kobayashi K
Organizer
38th Annual Meeting of Society for Neuroscience
Place of Presentation
Washington, D. C
Year and Date
2008-11-16
Related Report
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