Morphological study of the diacylglycerol kinase family
| Project/Area Number |
20700312
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Yamagata University |
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Principal Investigator |
HOZUMI Yasukazu Yamagata University, 医学部, 講師 (00372334)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ジアシルグリセロールキナーゼ / 免疫組織化学染色 / 特異抗体 / 共焦点レーザー顕微鏡 / 蛍光多重染色法 / ポストシナプス / 免疫電子顕微鏡法 / 細胞膜 |
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| Research Abstract |
DGKβ is expressed not only in projection neurons but also in interneurons and is concentrated at perisynaptic sites of asymmetrical synapses. Overexpression of wild-type DGKβ promotes dendrite outgrowth at 7 d in vitro (DIV) and spine maturation at 14 DIV in transfected hippocampal neurons, although its kinase-dead mutant has no effect. In addition, we show that DGKβ and dopamine D2 receptor are co-expressed in the intermediate lobe and localize to plasma membrane side by side. These findings will substantiate and further extend our understanding of the molecular-anatomical pathway of the phosphoinositide signaling and functional roles of DGK in the pituitary intermediate lobe.
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Report
(3 results)
Research Products
(11 results)
