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Identification of protein constituting inclusions from formalin-fixed paraffin-embedded fronto-temporal dementia brain tissue using laser-capture microdissection

Research Project

Project/Area Number 20700325
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Nerve anatomy/Neuropathology
Research InstitutionKansai Medical University

Principal Investigator

WATE Reika  Kansai Medical University, 医学部, 助教 (50440988)

Research Collaborator FUJITA Kengo  関西医科大学, 医学部, 大学院生
KINOSHITA Yoshimi  関西医科大学, 医学部, 大学院生
Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords神経変性疾患 / 神経病理学
Research Abstract

Basophilic Inclusions (BIs) in front-temporal dementia with BIs were separated and collected by laser capture micro-dissection from formalin-fixed paraffin-embedded brain tissue. The identified proteins by liquid chromatography coupled to mass spectrometry and concerning proteins were examined immunohistochemically. The BIs were distinctly labeled with the antibodies against microtubule-associated protein and autophagy-related gene proteins. Our results imply that autophagy has been disrupted before the completion of autophagosome formation, which might contribute to BI formation in this disorder.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (2 results)

All 2009

All Presentation (2 results)

  • [Presentation] 好塩基性封入体を伴う成人発症運動ニューロン病でのautophagy関連蛋白の免疫組織化学的検討2009

    • Author(s)
      藤田賢吾、金子鋭、伊東秀文、西井誠、中村正孝、木下芳美、朝山真哉、中村聖香、和手麗香、中野智、日下博文
    • Organizer
      第50回日本神経学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2009-05-21
    • Related Report
      2009 Final Research Report
  • [Presentation] 好塩基性封入体を伴う成人発症運動ニューロン病での autophagy 関連蛋白の免疫組織化学的検討2009

    • Author(s)
      藤田賢吾
    • Organizer
      第50回日本神経学会総会
    • Place of Presentation
      仙台国際センター(仙台)
    • Year and Date
      2009-05-21
    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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