| Project/Area Number |
20700325
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Nerve anatomy/Neuropathology
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
WATE Reika Kansai Medical University, 医学部, 助教 (50440988)
|
|---|
| Research Collaborator |
FUJITA Kengo 関西医科大学, 医学部, 大学院生
KINOSHITA Yoshimi 関西医科大学, 医学部, 大学院生
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 神経変性疾患 / 神経病理学 |
|---|
| Research Abstract |
Basophilic Inclusions (BIs) in front-temporal dementia with BIs were separated and collected by laser capture micro-dissection from formalin-fixed paraffin-embedded brain tissue. The identified proteins by liquid chromatography coupled to mass spectrometry and concerning proteins were examined immunohistochemically. The BIs were distinctly labeled with the antibodies against microtubule-associated protein and autophagy-related gene proteins. Our results imply that autophagy has been disrupted before the completion of autophagosome formation, which might contribute to BI formation in this disorder.
|
