Monitoring of CREB phosphorylation in single live neuron using split luciferase
| Project/Area Number |
20700331
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Neurochemistry/Neuropharmacology
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
ISHIMOTO Tetsuya University of Toyama, 大学院・医学薬学研究部(医学), 助教 (40397170)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | CREB / リン酸化 / ルシフェラーゼ / イメージング |
|---|
| Research Abstract |
cAMP response element binding protein (CREB) is a transcription factor which is activated by phosphorylation. We developed probe proteins that detected CREB phosphorylation using bioluminescence. In addition to the 1-molecule-type probe that was planned at the beginning of the project, we made 2-molecule-type probes. This approach increased the specificity of the probes.
|
Report
(4 results)
Research Products
(17 results)
