The functional analysis of key molecule in axon formation, CRMP-2

• Project/Area Number: 20700332
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Tamagawa University
• Principal Investigator: ARIMURA Nariko Tamagawa University, 脳科学研究所, グローバルCOE准教授 (20420375)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 神経伝達物質 / 受容体 / CRMP-2 / キネシン / ダイニン / 順行性輸送 / 逆行性輸送 / 軸索 / 神経 / 輸送 / TrkB

Research Abstract

The brain consists of the remarkably fine neural circuits. This circuits are made of axons, and CRMP-2 previously had been identified as a key molecule in axon formation. I analyzed the functional meaning of CRMP-2 in axon formation, and obtained the following results : 1) CRMP-2 regulates anterograde TrkB transport in axon through the association with Slp1 and Rab27. 2) CRMP-2 binds directly to cytoplasmic dynein, a motor protein of retrograde transport, and negatively regulates its function. These results suggest that CRMP-2 play a role in axon formation through regulating the molecular transport in axon.

Research Products

• [Journal Article] Direct interaction of Slp1 and Rab27 with TrkB receptor regulates its anterograde transport in axons (2009)
  • Author(s): Arimura N, Kimura T, Nakamuta, S, Taya S, Funahashi Y, Hattori A, Shimada A, Menager C, Kawabata S, Fujii K, Iwamatsu A, Segal RA, Fukuda M, Kaibuchi K
  • Journal Title: Developmental Cell 16 Pages: 675-86
  • Peer Reviewed
• [Journal Article] CRMP-2 directly binds to cytoplasmic dynein and interferes with its activity (2009)
  • Author(s): Arimura N, Hattori A, Kimura T, Nakamuta S, Funahashi Y, Hirotsune S, Furuta K, Urano T, Toyoshima YY, Kaibuchi K
  • Journal Title: Journal of Neurochemistry 111(2) Pages: 380-90
  • Peer Reviewed
• [Journal Article] TDP-43 depletion induces neuronal cell damage through dysregulation of Rho family GTPases (2009)
  • Author(s): Iguchi Y, Katsuno M, Niwa J, Yamada S, Sone J, Waza M, Adachi H, Tanaka F, Nagata K, Arimura N, Watanabe T, Kaibuchi K, Sobue G
  • Journal Title: Journal of Biological Chemistry 284 Pages: 22059-66
  • Peer Reviewed
• [Journal Article] Direct interaction of Slp1 and Rab27 with TrkB receptor regulates its anterograde transport in axons. (2009)
  • Author(s): Arimura N
  • Journal Title: Journal of Neurochemistry 111 Pages: 380-90
  • Peer Reviewed
• [Journal Article] TDP-43 depletion induces neuronal cell damage through dysregulation of Rho family GTPases. (2009)
  • Author(s): Iguchi Y
  • Journal Title: Journal of Biological Chemistry 284 Pages: 22059-66
  • Peer Reviewed
• [Journal Article] Direct interaction of Slpl and Rab27 with TrkB receptor regulates its anterograde transport in axons (2009)
  • Author(s): Arimura. N, et.al.
  • Journal Title: Dev. Cell 6 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Neurofibromatosis type 1 (NF1) tumor suppressor, neurofibromin, regulates the neuronal differentiation of PC12 cells via its associating protein, CRMP-2 (2008)
  • Author(s): Patrakitkomjorn S, Kobayashi D, Morikawa T, Wilson MM, Tsubota N, Irie A, Ozawa T, Aoki M, Arimura N, Kaibuchi K, Saya H, Araki N
  • Journal Title: Journal of Biological Chemistry 283 Pages: 9399-413
  • Peer Reviewed
• [Journal Article] NF1 tumor suppressor, neurofibromin, regulates the neuronal differentiation of PC12 cells via its associating protein, collapsin response mediator protein-2 (2008)
  • Author(s): Patrakitkomjorn. S, et.al.
  • Journal Title: J. Biol. Chem 283 Pages: 9399-9413
  • Peer Reviewed
• [Presentation] Involvement of subareas within the dorsal premotor area (PMd) in a conceptually demanding visuomotor task (2009)
  • Author(s): Nakayama Y, Arimura N, Yamagata T, Tanji J, Hoshi E
  • Organizer: Neuroscience2009
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-19
• [Presentation] Involvement of subareas within the dorsal premotor area (PMd) in a conceptually demanding visuomotor task. (2009)
  • Author(s): Nakayama Y
  • Organizer: Neuroscience2009
  • Place of Presentation: McCormick Place(Chicago, USA)
  • Year and Date: 2009-10-19
• [Presentation] プロテオミクス解析による、Slp1相互作用タンパク質としてPI 3-kinaseの同定 (2009)
  • Author(s): Funahashi Y, Arimura N, Nakamuta S, Kaibuchi K
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-18
• [Presentation] Identification of Slpl-interacting-protein in neuron (2008)
  • Author(s): 船橋靖広
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 兵庫県、神戸国際会議場
  • Year and Date: 2008-12-11
• [Presentation] Identification of Slp1-interactingprotein in neuron (2008)
  • Author(s): Funahashi Y, Arimura N, Kaibuchi K
  • Organizer: BMB2008
  • Place of Presentation: Kobe
  • Year and Date: 2008-12-09
• [Presentation] Rab27 and Slp1 regulate anterograde transport of TrkB receptors in axons (2008)
  • Author(s): 有村奈利子、中牟田信一、船橋靖広、貝淵弘三
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-06-29
• [Presentation] Rab27 and Slpl regulate anterograde transport of TrkB receptors in axons (2008)
  • Author(s): 有村奈利子
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 兵庫県、神戸国際会議場
  • Year and Date: 2008-06-29
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/Yakuri/