Maintenance and self-renewal mechanism of muscle satellite cells

• Project/Area Number: 20700358
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Osaka University
• Principal Investigator: FUKADA So-ichiro Osaka University, 薬学研究科, 助教 (20432445)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 骨格筋生理・病態学 / 筋衛星細胞 / 維持機構 / カルシトニン受容体 / 幹細胞 / 骨格筋 / ニッチ / 再生 / ニッチェ

Research Abstract

I investigated the roles of quiescent satellite cell-specific genes in vitro and in vivo. One of specific genes, calcitonin receptor suppressed proliferation and migration of satellite cells in vitro. In addition, hesr1 and 3 double knock mice exhibited the decreased number of muscle satellite cells in vivo. Furthermore self-renewal potential of muscle satellite cells were different among mouse strain, and the potential were directly related to muscle weight.

Research Products

• [Journal Article] Genetic background affects properties of satellite cells and mdx phenotypes (*corresponding author). (2010)
  • Author(s): Fukada S*, Morikawa D, Yamamoto Y, Yoshida T, Sumie N, Yamaguchi M, Ito T, Miyagoe-Suzuki Y, Takeda S, Tsujikawa K, Yamamoto H
  • Journal Title: American Journal of Pathology (in press)
  • Peer Reviewed
• [Journal Article] Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells (2010)
  • Author(s): Mizuno Y, Chang H, Umeda K, Niwa A, Iwasa T, Awaya T, Fukada S, Yamamoto H, Yamanaka S, Nakahata T, Heike T
  • Journal Title: FASEB J (in press)
  • Peer Reviewed
• [Journal Article] Mesenchymal progenitors distinct from muscle satellite cells contribute to ectopic fat cell formation in skeletal muscle. (2010)
  • Author(s): Uezumi A, Fukada S, Yamamoto N, Takeda S, Tsuchida K
  • Journal Title: Nature Cell Biology 12(2) Pages: 143-52
  • Peer Reviewed
• [Journal Article] Mesenchymal progenitors distinct from muscle satellite cells contribute to ectopic fat cell formation in skeletal muscle. (2010)
  • Author(s): 上住聡芳, 等
  • Journal Title: Nature Cell Biology 12 Pages: 143-152
  • Peer Reviewed
• [Journal Article] 骨格筋重量維持機構と筋衛星細胞 (2009)
  • Author(s): 深田宗一朗, 山元弘
  • Journal Title: 基礎老化学会誌 33(4) Pages: 13-15
  • NAID: 10026103769
  • Peer Reviewed
• [Journal Article] Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells. (2009)
  • Author(s): Chang H, Yoshimoto M, Umeda K, Iwasa T, Mizuno Y, Fukada S, Yamamoto H, Motohashi N, Suzuki YM, Takeda S, Heike T, Nakahata T
  • Journal Title: FASEB J. 23(6) Pages: 1907-19
  • Peer Reviewed
• [Journal Article] 骨格筋重量維持機構と筋衛星細胞 (2009)
  • Author(s): 深田宗一朗, 等
  • Journal Title: 日本基礎老化学会誌 33 Pages: 13-15
  • NAID: 10026103769
• [Journal Article] Suppression of macrophage functions impairs skeletal muscle regeneration with severe fibrosis. (*corresponding author) (2008)
  • Author(s): Segawa M, Fukada S*, Yamamoto Y, Yahagi H, Kanematsu M, Sato M, Ito T, Uezumi A, Hayashi S, Miyagoe-Suzuki Y, Takeda S, Tsujikawa K, Yamamoto H
  • Journal Title: Exp. Cell Res. 314 Pages: 3232-3244
  • Peer Reviewed
• [Journal Article] Muscle CD31(-)CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts. (2008)
  • Author(s): Motohashi N, Uezumi A, Yada E, Fukada S, Fukushima K, Imaizumi K, Miyagoe-Suzuki Y, Takeda S
  • Journal Title: American Journal of Pathology 173 Pages: 781-91
  • Peer Reviewed
• [Journal Article] Suppression of macrophage functions impairs skeletal muscle regeneration with severe fibrosis (2008)
  • Author(s): 瀬川将司, 等
  • Journal Title: Experimental Cell Research 314 Pages: 3232-3244
  • Peer Reviewed
• [Journal Article] Muscle CD31(-)CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts (2008)
  • Author(s): 本橋紀夫, 等
  • Journal Title: American Journal of Pathology 173 Pages: 781-91
  • Peer Reviewed
• [Journal Article] Genetic background affects properties of satellite cells and mdx phenotypes
  • Author(s): 深田宗一朗, 等
  • Journal Title: American Journal of Pathology (印刷中)
  • Peer Reviewed
• [Journal Article] Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells
  • Author(s): Mizuno Y, 等
  • Journal Title: FASEB Journal (印刷中)
  • Peer Reviewed
• [Journal Article] Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells
  • Author(s): Chang H, 等
  • Journal Title: FASEB Journal (印刷中)
  • Peer Reviewed
• [Presentation] Properties of satellite cells and mdx phenotype are influenced by genetic backgrounds (2009)
  • Author(s): 深田宗一朗, 等
  • Organizer: 第7回国際幹細胞学会
  • Place of Presentation: スペイン・バルセロナ・バルセロナ国際コンベンションセンター
  • Year and Date: 2009-07-09
• [Presentation] Properties of satellite cells and mdx phenotype are influenced by genetic backgrounds (2009)
  • Author(s): So-ichiro Fukada, Daisuke Morikawa, Yuko Miyagoe-Suzuki, Shin'ichi Takeda, Kazutake Tsujikawa, Hiroshi Yamamoto
  • Organizer: 第7回国際幹細胞学会
  • Place of Presentation: バルセロナ・スペイン
• [Book] Muscle cell physiology(Edited by Yoshinobu Ohira, Macrophages and skeletal muscle regeneration.) (2009)
  • Author(s): So-ichiro Fukada, Hiroshi Yamamoto
• [Book] Muscle cell physiology (2009)
  • Author(s): 深田宗一朗, 等
  • Total Pages: 12
  • Publisher: Osaka University Press
• [Book] 難病と在宅ケア(筋衛星細胞の維持、活性化と自己複製の制御機構) (2008)
  • Author(s): 深田宗一朗, 等
  • Total Pages: 3
  • Publisher: (株)日本プランニングセンター