Establishment and application of the library for in vivo imaging in living mice.

• Project/Area Number: 20700361
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Hokkaido University
• Principal Investigator: TSUKIYAMA Tadasuke Hokkaido University, 大学院・医学研究科, 助教 (20399819)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 動物実験技術 / マウス遺伝学 / がん / 免疫 / シグナル伝達 / イメージング

Research Abstract

We have established and reported for cNAT system, which can detect the activity of NF-κB signaling. Next, we generated cNAT reporter transgenic mice and cNotch reporter system/transgenic mice to detect NF-κB signaling and Notch signaling respectively, in living mice.

Research Products

• [Journal Article] TRIM24 mediates ligand-dependent activation of androgen receptor and is repressed by a bromodomain-containing protein, BRD7, in prostate cancer cells. (2009)
  • Author(s): Kikuchi M, Okumura F, Tsukiyama T, Watanabe M, Miyajima N, Tanaka J, Imamura M, Hatakeyama S
  • Journal Title: Biochim Biophys Acta. 1793(12) Pages: 1828-36
  • NAID: 120001804922
  • Peer Reviewed
• [Journal Article] TRIM31 interacts with p52(Shc) and inhibits Src-induced anchorage -independent growth. (2009)
  • Author(s): Watanabe M, Tsukiyama T, Hatakeyama S
  • Journal Title: Biochem Biophys Res Commun 388(2) Pages: 422-7
  • NAID: 120001896687
  • Peer Reviewed
• [Journal Article] Ubiquitin-conjugating enzyme UBE2Q2 suppresses cell proliferation and is down-regulated in recurrent head and neck cancer. (2009)
  • Author(s): Maeda H, Miyajima N, Kano S, Tsukiyama T, Okumura F, Fukuda S, Hatakeyama S
  • Journal Title: Mol Cancer Res. 7(9) Pages: 1553-62
  • Peer Reviewed
• [Journal Article] Wnt2/2b and beta-catenin signaling are necessary and sufficient to specify lung progenitors in the foregut. (2009)
  • Author(s): Goss AM, Tian Y, Tsukiyama T, Cohen ED, Zhou D, Lu MM, Yamaguchi TP, Morrisey EE
  • Journal Title: Developmental Cell 17(2) Pages: 290-8
  • Peer Reviewed
• [Journal Article] Wnt2/2b and β-Catenin Signaling Are Necessary and Sufficient to Specify Lung Progenitors in the Foregut (2009)
  • Author(s): Ashley M.Goss
  • Journal Title: Developmental Cell 17 Pages: 290-298
  • Peer Reviewed
• [Journal Article] TRIM24 mediates ligand-dependent activation of androgen receptor and is repressed by a bromodomain-containing protein, BRD7, in prostate cancer cells (2009)
  • Author(s): Kikuchi M
  • Journal Title: Biochimica et Biophysica Acta 1793(12) Pages: 1828-1836
  • Peer Reviewed
• [Journal Article] Ubiquitin-conjugating enzyme UBE2Q2 suppresses cell proliferation and is down-regulated in recurrent head and neck cancer (2009)
  • Author(s): Maeda H
  • Journal Title: Molecular Cancer Research 7(9) Pages: 1553-1562
  • Peer Reviewed
• [Journal Article] TRIM31 interacts with p52^<Shc> and inhibits Src-induced anchorage-independent growth (2009)
  • Author(s): Masashi Watanabe
  • Journal Title: Biochemical and Biophysical Research Communications 388(2) Pages: 422-427
  • Peer Reviewed
• [Journal Article] Embryonic hair follicle fate change by augmented beta-catenin through Shh and Bmp signaling (2009)
  • Author(s): Suzuki K, Yamaguchi Y, Villacorte M, Mihara K, Akiyama M, Shimizu H, Taketo MM, Nakagata N, Tsuk i yama T, Yamaguchi TP, Birchmeier W, Kato S. Yamada G
  • Journal Title: Developmerlt 136(3) Pages: 367-372
  • Peer Reviewed
• [Journal Article] Inhibition of NF-kappaB signaling via tyrosine phosphorylation of Ymer (2009)
  • Author(s): Kameda H, Watanabe M, Bohgaki M, Tsukiyama T, Hatakeyama S
  • Journal Title: Biochemical and Biophysical Research Communi cations 378(4) Pages: 744-749
  • Peer Reviewed
• [Journal Article] Involvement of Ymer in suppression of NF-kappaB activation by regulated interaction with lysine-63-linked polyubiquitin chain (2008)
  • Author(s): Bohgaki M, Tsukiyama T, Nakajima A, Maruyama S, Watanabe M, Koike T, Hatakeyama S
  • Journal Title: Biochimica et Biophysica Acta 1783(5) Pages: 826-837
  • Peer Reviewed
• [Journal Article] Ro52 functionally interacts with IgG1 and regulates its quality control via the ERAD system (2008)
  • Author(s): Takahata M, Bohgaki M, Tsukiyama T, Kondo T. Asaka M. Hatakevama S
  • Journal Title: Molecullar Immunology 45(7) Pages: 2045-2054
  • Peer Reviewed
• [Presentation] NF-κBシグナルを抑制するYmerトランスジェニックマウスの解析 (2009)
  • Author(s): 築山忠維・坊垣幸・畠山鎮次
  • Organizer: 平成21年度・特定領域研究「タンパク質分解」班会議
  • Place of Presentation: 滋賀・長浜ロイヤルホテル
  • Year and Date: 2009-12-21
• [Presentation] NF-κB抑制分子 Ymer トランスジェニックマウスの解析 (2009)
  • Author(s): 築山忠維
  • Organizer: 平成21年度・特定領域研究「タンパク質分解」班会議
  • Place of Presentation: 滋賀・長浜ロイヤルホテル
  • Year and Date: 2009-12-21
• [Presentation] 、NF-κB抑制分子Ymerトランスジェニックマウスの解析 (2009)
  • Author(s): 築山忠維・坊垣幸・畠山鎮次
  • Organizer: 日本分子生物学会・第32回年会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] NF-κB抑制分子 Ymer トランスジェニックマウスの解析 (2009)
  • Author(s): 築山忠維
  • Organizer: 日本分子生物学会・第32回年会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] がん抑制遺伝子としてのWnt5a (2009)
  • Author(s): 築山忠維
  • Organizer: 九州大学COE「個体恒常性を担う細胞運命の決定とその破綻」/SSP「生体防御におけるポストゲノムサイエンス」共催シンポジウムWntシグナルの機能と抑制 : 基礎研究から再生医療、がん治療へ」
  • Place of Presentation: 福岡・九州大学
  • Year and Date: 2009-03-24
• [Presentation] 自己免疫疾患に関与するユビキチン関連分子の治療に向けた応用 (2008)
  • Author(s): 築山忠維・坊垣幸・畠山鎮次
  • Organizer: 厚生労働科学研究費補助金免疫・アレルギー疾患予防・治療研究事業「免疫疾患に対する免疫抑制療法等先端的新規治療法に関する研究」班会議
  • Place of Presentation: 札幌・北海道大学
  • Year and Date: 2008-12-04
• [Presentation] NF-κB抑制分子Ymerトランスジェニックマウスの解析 (2008)
  • Author(s): 築山忠維・坊垣幸・畠山鎮次
  • Organizer: 平成20年度・特定領域研究「タンパク質分解」班会議
  • Place of Presentation: 筑波・つくばグランドホテル
  • Year and Date: 2008-11-26
• [Presentation] NF-κB抑制分子Ymerトランスジェニックマウスの解析 (2008)
  • Author(s): 築山忠維・松田真由子・畠山鎮次
  • Organizer: 第73回日本インターフェロン・サイトカイン学会学術集会
  • Place of Presentation: 札幌・北海道大学
  • Year and Date: 2008-07-11
• [Presentation] NF-κB抑制分子Ymerトランスジェニックマウスの解析 (2008)
  • Author(s): 築山忠維・坊垣幸・畠山鎮次
  • Organizer: 日本分子生物学会・第31回年会
  • Place of Presentation: 神戸・ポートアイランド