| Project/Area Number |
20700365
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Tottori University |
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Principal Investigator |
OOBAYASHI Tetsuya Tottori University, 生命機能研究支援センター, 准教授 (80348804)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | リコンビナントタンパク質 / 弾性線維 / 人工染色体 / 遺伝子改変動物 / 個体老化 / 動脈硬化 / 肺気腫 / 皮膚の弛緩 |
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| Research Abstract |
To generate recombinant-DANCE protein, we utilised a state-of-the-art artificial chromosomal technology. This technology enables us to introduce a gene on a human artificial chromosome (HAC) vector (developed at Tottori University), thus the transgene can be of a virtually unlimited length, including all native regulatory regions. As pilot study, a protein expression vector was introduced into the artificial chromosome vector of CHO cell, and that protein developed efficiently and stably. To establish the animal model, I utilise a transchromosomic (TC) mouse technology, which is derived from a combination of artificial chromosomal and developmental engineering. I developed the multiple-gene inducible mouse EScells using HAC vector system and multiple-integrase.
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