The mechanism of developing age-dependent disease by loss of elasticity.

• Project/Area Number: 20700365
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Tottori University
• Principal Investigator: OOBAYASHI Tetsuya Tottori University, 生命機能研究支援センター, 准教授 (80348804)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: リコンビナントタンパク質 / 弾性線維 / 人工染色体 / 遺伝子改変動物 / 個体老化 / 動脈硬化 / 肺気腫 / 皮膚の弛緩

Research Abstract

To generate recombinant-DANCE protein, we utilised a state-of-the-art artificial chromosomal technology. This technology enables us to introduce a gene on a human artificial chromosome (HAC) vector (developed at Tottori University), thus the transgene can be of a virtually unlimited length, including all native regulatory regions. As pilot study, a protein expression vector was introduced into the artificial chromosome vector of CHO cell, and that protein developed efficiently and stably. To establish the animal model, I utilise a transchromosomic (TC) mouse technology, which is derived from a combination of artificial chromosomal and developmental engineering. I developed the multiple-gene inducible mouse EScells using HAC vector system and multiple-integrase.

Research Products

• [Journal Article] Fibulin-4 Conducts Proper Elastogenesis via Interaction with Cross-linking Enzyme Lysyl Oxidase (2009)
  • Author(s): Horiguchi M, Inoue T, Ohbayashi T, Hirai M, Noda K, Marmorstein LY, Yabe D, Takagi K, Akama TO, Kita T, Kimura T, Nakamura T
  • Journal Title: Proc Natl Acad Sci U S A. 106 Pages: 19029-19034
  • Peer Reviewed
• [Journal Article] Fibulin-4 conducts proper elastogenesis via interaction with cross-linking enzyme lysyl oxidase (2009)
  • Author(s): Horiguchi M, Inoue T, Ohbayashi T, Hirai M, Noda K, Marmorstein LY, Yabe D, Takagi K, Akama T O, Kita T, Kimura T, Nakamura T.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 19029-19034
  • Peer Reviewed
• [Journal Article] Immunohistochemical investigation of mid-dermal elastolysis with a history of erythema. (2008)
  • Author(s): Suda T, Hara H, Yoshitake M, Ohbayashi T,, Nakamura T, Terui T.
  • Journal Title: Am J Dermatopathol. 30(5) Pages: 477-80
  • Peer Reviewed
• [Journal Article] Immunohistochemical investigation of mid-dermal elastolysis with a history of erythema (2008)
  • Author(s): Suda T, Hara H, Yoshitake M, Ohbayashi T, Nakamura T, Terui T.
  • Journal Title: American Jurnal of Dermatopathol 30 Pages: 477-480
  • Peer Reviewed
• [Presentation] 遺伝子改変動物を用いた弾性線維形成におけるDANCEプロセッシングの検討 (2009)
  • Author(s): 堀口真仁, 大林徹也, 中邨智之
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮市
  • Year and Date: 2009-05-14
• [Presentation] DANCEは弾性線維形成のオーガナーザー因子であり、欠損したマウスは加齢進行性疾患の表現系を示す (2009)
  • Author(s): 大林徹也, 堀口真仁, 中邨智之
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮市
  • Year and Date: 2009-05-14