Analysis of genomic reprogramming in mammals by utilizing inactive X chromosome.

• Project/Area Number: 20700366
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Tottori University
• Principal Investigator: NAKANISHI Tomoko Tottori University, 医学部, 助教 (10344863)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: GFP / X染色体 / 不活性 / 再プログラム化 / MacroH2A / エピゲノム変化 / クロマチン構造 / 未受精卵

Research Abstract

One of the sex chromosome, X chromosome, changes its chromatin structure during genomic reprogramming in mammalian females. We produced a construct that intensely labels inactive X chromosome by expressing GFP protein fused with MacroH2A that localizes abundantly on inactive X chromosome. It was able to trace the chromatin structure of X chromosome in culture cells and mouse early embryos by utilizing the construct. Our system would enable us to examine the process of genomic reprogramming in germ-line cells and oocytes.

Research Products

• [Journal Article] Functional roles of sperm hyaluronidases, HYAL5 and SPAM1, in fertilization in mice. (2009)
  • Author(s): Kimura, M., Kim, E., Kang, W., Yamashita, M., Saigo, M., Yamazaki, T., Nakanishi, T., Kashiwabara, S.I., Baba, T.
  • Journal Title: Biol Reprod. 81 Pages: 939-947
  • Peer Reviewed
• [Journal Article] Establishment and characterization of hepatic stem-like cell lines from normal adult rat liver. (2009)
  • Author(s): Hirata, M., Amano, K., Miyashita, A., Yasunaga, M., Nakanishi T., Sato, K.
  • Journal Title: J. Biochem. 145 Pages: 51-58
  • NAID: 10024914364
  • Peer Reviewed
• [Journal Article] Functional roles of sperm hyaluronidases, HYAIS and SPAM1, in fertilization in mice. (2009)
  • Author(s): Kimura, M., Kim, E.., Kang, W., Yamashita, M., Saigo, M., Yamazaki, T., Nakanishi T., et al.
  • Journal Title: Biol.Reprod 81 Pages: 939-947
  • Peer Reviewed
• [Journal Article] Establishment and Characterization of Hepatic Stem-like Cell lines from Normal Adult Rat Liver (2009)
  • Author(s): Hirata, M., Amano, K., Miyashita, A., Yasunaga, M., Nakanishi T., Sato. K.
  • Journal Title: J. Biochem. 145 Pages: 51-58
  • NAID: 10024914364
  • Peer Reviewed
• [Journal Article] Birth of normal offspring from mouse eggs activated by a phospholipase Cζprotein lacking three EF-hand domains. (2008)
  • Author(s): Nakanishi, T., Ishibashi, N., Kubota, H., Inoue, K., Ogonuki, N., Ogura, A., Kashiwabara, S., Baba, T.
  • Journal Title: J. Reprod. Dev. 54 Pages: 244-249
  • NAID: 10024289241
  • Peer Reviewed
• [Journal Article] Birth of normal offspring from mouse eggs activated by a phospholipase Cζ protein lacking three EF-hand domains (2008)
  • Author(s): Nakanishi. T., et.al.
  • Journal Title: J. Reprod. Dev. 54 Pages: 244-249
  • NAID: 10024289241
  • Peer Reviewed
• [Presentation] MacroH2A-EGFPを利用したマウス初期胚における不活性X染色体の追跡 (2009)
  • Author(s): 相馬淳美、中西友子、前田康彰、国分一男、堀直裕、佐藤建三
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 兵庫
  • Year and Date: 2009-12-12
• [Presentation] MacroH2A-EGFPを利用した生殖系列細胞における不活性X染色体の標識 (2009)
  • Author(s): 相馬淳美、中西友子、国分一男、堀直裕、佐藤建三
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2009-12-12
• [Presentation] MacroH2A-EGFPを利用したマウス初期胚における不活性X染色体の追跡 (2009)
  • Author(s): 相馬淳美, 中西友子, 前田康彰, 国分一男, 堀直裕, 佐藤建三
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 兵庫県
  • Year and Date: 2009-12-09
• [Presentation] マウス初期胚における脱メチル化能の解析 (2009)
  • Author(s): 中西友子, 前田康彰, 相馬淳美, 堀直裕, 佐藤建三
  • Organizer: 特定領域研究生殖サイクル若手勉強会2009
  • Place of Presentation: 静岡県
  • Year and Date: 2009-08-27
• [Presentation] 生殖系列細胞における不活性X染色体の追跡 (2009)
  • Author(s): 相馬淳美, 中西友子, 国分一男, 堀直裕, 佐藤建三
  • Organizer: 特定領域研究生殖サイクル若手勉強会2009
  • Place of Presentation: 静岡県御
  • Year and Date: 2009-08-27
• [Presentation] リアルタイム骨形成活性評価を目指したトランスジェニックマウスの作製 (2009)
  • Author(s): 国分一男、中西友子、相馬淳美、森島未帆, 等
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 埼玉
  • Year and Date: 2009-05-15
• [Presentation] BMP-4発現モニターできるトランスジェニックマウスの作製とin vivo imagingによる膵臓機能の評価 (2009)
  • Author(s): 安永茉由、中西友子、香月康宏、押村光雄、佐藤建三
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 埼玉
  • Year and Date: 2009-05-15
• [Presentation] MacroH2A-EGFPを利用した生殖系列細胞における不活性X染色体の標識 (2008)
  • Author(s): 相馬淳美, 中西友子, 国分一男, 堀直裕, 佐藤建三
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 兵庫県
  • Year and Date: 2008-12-09
• [Presentation] MacroH2Aを利用した不活性化X染色体の標識 (2008)
  • Author(s): 相馬淳美, 中西友子, 国分一男, 堀直裕, 佐藤建三
  • Organizer: 第1回生殖系列の世代サイクルとエピゲノムネットワーク」「第5回性分化機構の解明」合同領域会議
  • Place of Presentation: 熊本県
  • Year and Date: 2008-11-24
• [Presentation] MacroH2Aを利用した不活性化X染色体の標識 (2008)
  • Author(s): 相馬淳美、中西友子、国分一男、堀直裕、佐藤建三
  • Organizer: 「第1回生殖系列の世代サイクルとエピゲノムネットワーク」「性分化機構の解明」合同領域会議
  • Place of Presentation: 熊本
  • Year and Date: 2008-11-24
• [Remarks]
  • URL: http://www.med.tottori-u.ac.jp/p/igaku/gakka/seimei/bunshi/bunshi_home/