| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
One of the sex chromosome, X chromosome, changes its chromatin structure during genomic reprogramming in mammalian females. We produced a construct that intensely labels inactive X chromosome by expressing GFP protein fused with MacroH2A that localizes abundantly on inactive X chromosome. It was able to trace the chromatin structure of X chromosome in culture cells and mouse early embryos by utilizing the construct. Our system would enable us to examine the process of genomic reprogramming in germ-line cells and oocytes.
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