Study on sperm preservation at room temperature in the rodents
| Project/Area Number |
20700367
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Kumamoto University |
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Principal Investigator |
KANEKO Takehito Kumamoto University, 生命資源研究部・支援センター, 助教 (30332878)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | マウス / ラット / 精子 / 保存 / ICSI / フリーズドライ |
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| Research Abstract |
Sperm preservation at room temperature is a powerful tool for bio-resource projects. Freeze-drying is a method which can preserve and transport sperm at room temperature without using liquid nitrogen. We studied preservation of sperm at room temperature by using freeze-drying. The fertility of freeze-dried sperm was maintained after preservation for 1 year at 4 oC. Offspring was obtained from oocytes fertilized with these sperm. Moreover, this study was described that normality of sperm chromatin is an important factor to preserve sperm at room temperature.
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Report
(3 results)
Research Products
(17 results)
