Study on sperm preservation at room temperature in the rodents

• Project/Area Number: 20700367
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Kumamoto University
• Principal Investigator: KANEKO Takehito Kumamoto University, 生命資源研究部・支援センター, 助教 (30332878)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: マウス / ラット / 精子 / 保存 / ICSI / フリーズドライ

Research Abstract

Sperm preservation at room temperature is a powerful tool for bio-resource projects. Freeze-drying is a method which can preserve and transport sperm at room temperature without using liquid nitrogen. We studied preservation of sperm at room temperature by using freeze-drying. The fertility of freeze-dried sperm was maintained after preservation for 1 year at 4 oC. Offspring was obtained from oocytes fertilized with these sperm. Moreover, this study was described that normality of sperm chromatin is an important factor to preserve sperm at room temperature.

Research Products

• [Journal Article] Importance of primary culture conditions for the development of rat ICSI embryos and long-term preservation of freeze-dried sperm. (2009)
  • Author(s): Takehito Kaneko, Shinya Kimura, Naomi Nakagata
  • Journal Title: Cryobiology 58 Pages: 293-297
  • Peer Reviewed
• [Journal Article] Importance of primary culture conditions for the development of rat ICSI embryos and long term preservation of freeze-dried sperm (2009)
  • Author(s): Takehito Kaneko
  • Journal Title: Cryobiology 58 Pages: 293-297
  • Peer Reviewed
• [Journal Article] Importance of primary culture conditions for the development of rat ICSI embryos and long term preservation of freeze-dried sperm
  • Author(s): Takehito Kaneko
  • Journal Title: Cryobiology (In press)
  • Peer Reviewed
• [Presentation] The development of oocytes injected with freeze-dried rat sperm (2009)
  • Author(s): Takehito Kaneko
  • Organizer: Cryo2009, 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: 北海道 北海道大学
  • Year and Date: 2009-07-19
• [Presentation] Tolerance to freezing and maintenance of fertilizing ability in rat sperm (2009)
  • Author(s): Takehito Kaneko
  • Organizer: Cryo2009, 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: 北海道 北海道大学
  • Year and Date: 2009-07-19
• [Presentation] Gamete preservation for resource banking (2009)
  • Author(s): Takehito Kaneko
  • Organizer: 21^<st> Annual Scientific Conference & 4^<th> Laboratory Animal Workshop
  • Place of Presentation: Alabang Muntinlupa city, Philippine
  • Year and Date: 2009-05-21
• [Presentation] Resource banking and fertilization technuiques (2009)
  • Author(s): Takehito Kaneko
  • Organizer: 21^<st> Annual Scientific Conference & 4^<th> Laboratory Animal Workshop
  • Place of Presentation: Alabang Muntinlupa city, Philippine
  • Year and Date: 2009-05-21
• [Presentation] ラット精巣内精子の凍結保存および産子の作出 (2009)
  • Author(s): 金子武人
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 埼玉県 大宮ソニックシティ
  • Year and Date: 2009-05-14
• [Presentation] ラット精子の保存と受精技術の開発 (2009)
  • Author(s): 金子武人
  • Organizer: 第2回ラットリソースリサーチ研究会
  • Place of Presentation: 京都府
  • Year and Date: 2009-01-30
• [Presentation] ラット精巣内精子の凍結保存および産子の作出 (2009)
  • Author(s): 金子武人
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 日埼玉県大宮市大宮ソニックシティ
• [Presentation] Gamete preservation for resource banking. (2009)
  • Author(s): Takehito Kaneko
  • Organizer: 21st Annual Scientific Conference & 4th Laboratory Animal Workshop.
  • Place of Presentation: Alabang Muntinlupa city, Philippine
• [Presentation] Resource banking and fertilization techniques. (2009)
  • Author(s): Takehito Kaneko
  • Organizer: 21st Annual Scientific Conference & 4th Laboratory Animal Workshop
  • Place of Presentation: Alabang Muntinlupa city, Philippine
• [Presentation] The development of oocytes injected with freeze-dried rat sperm. (2009)
  • Author(s): Takehito Kaneko
  • Organizer: Cryo2009: 46th Annual Meeting of the Society for Cryobiology.
  • Place of Presentation: Sapporo, Hokkaido
• [Presentation] Tolerance to freezing and maintenance of fertilizing ability in rat sperm. (2009)
  • Author(s): Takehito Kaneko
  • Organizer: Cryo2009: 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Sapporo, Hokkaido
• [Presentation] Development of new sperm preservation method by freeze-drying in rodents (2008)
  • Author(s): Takehito Kaneko
  • Organizer: The 3^<th> AFLAS Congress & The 8^<th> CALAS Annual Meeting
  • Place of Presentation: 中国北京市
  • Year and Date: 2008-09-27
• [Presentation] ラットフリーズドライ精子の長期保存後の受精能とICSI胚の培養条件の検討 (2008)
  • Author(s): 金子武人
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡県
  • Year and Date: 2008-09-18
• [Presentation] ラット精子の核構造がICSI後の胚発生に及ぼす影響について (2008)
  • Author(s): 金子武人
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡県
  • Year and Date: 2008-09-18