| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
This study established a targeted transgenesis system based on Cre-loxP site-specific recombination in fertilized eggs, but not in ES cells. A transgene, flanked by two mutant loxP sites, was integrated into target loci in the seed mice (containing loxP-site derivatives at Rosa26 locus) by recombinase-mediated cassette exchange (RMCE) in fertilized eggs. By applying this method, we have established a total of 25 transgenic lines showing ubiquitous, strong, and reproducible transgene expression. We also demonstrated that knockdown mice can be readily generated by PITT by taking advantage of our reproducible and highly efficient expression system.
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