Development and application of novel transgenic technology by in vivo RMCE
Project/Area Number |
20700368
|
Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Laboratory animal science
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Research Institution | Tokai University |
Principal Investigator |
OHTSUKA Masato Tokai University, 医学部, 講師 (90372945)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | Cre-loxPシステム / RMCE法 / トランスジェニックマウス / 蛍光遺伝子 / 顕微注入法 / Rosa26遺伝子座位 / ノックダウン / Cre-loxpシステム / 疾患感受性遺伝子 |
Research Abstract |
This study established a targeted transgenesis system based on Cre-loxP site-specific recombination in fertilized eggs, but not in ES cells. A transgene, flanked by two mutant loxP sites, was integrated into target loci in the seed mice (containing loxP-site derivatives at Rosa26 locus) by recombinase-mediated cassette exchange (RMCE) in fertilized eggs. By applying this method, we have established a total of 25 transgenic lines showing ubiquitous, strong, and reproducible transgene expression. We also demonstrated that knockdown mice can be readily generated by PITT by taking advantage of our reproducible and highly efficient expression system.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.2010
Author(s)
Ohtsuka M., Ogiwara S., Miura H., Mizutani A., Warita T., Sato M., Imai K., Hozumi K., Sato T., Tanaka M., Kimura M., Inoko H.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in the mouse genome.2009
Author(s)
Kokubu C., Horie K., Abe K., Ikeda R., Mizuno S., Uno Y., Ogiwara S., Ohtsuka M., Isotani A., Okabe M., Imai K., Takeda J.
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Journal Title
Nat.Genet. 41
Pages: 946-952
Related Report
Peer Reviewed
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