Construction of cellular imaging system by delivery of antibody into living cells

• Project/Area Number: 20700376
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: MIE Masayasu Tokyo Institute of Technology, 大学院・生命理工学研究科, 助教 (40334528)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 抗体 / イメージング / 抗体導入 / luciferase / 細胞内分子

Research Abstract

The purpose of this experiment was construction of imaging system in living cell by delivery of antibody. We have constructed imaging system which shows the signal when antibody bind to a target molecule. It was showed that our imaging system would be applied for living cell imaging.

Research Products

• [Journal Article] RNA detection using peptide-inserted Renilla luciferase. (2009)
  • Author(s): Andou T, Endoh T, Mie M, Kobatake E
  • Journal Title: Anal Bioanal Chem. 393 Pages: 661-8
  • Peer Reviewed
• [Journal Article] Transduction of MyoD protein into myoblasts induces myogenic differentiation without addition of protein transduction domain. (2009)
  • Author(s): Noda T, Fujino T, Mie M, Kobatake E
  • Journal Title: Biochem Biophys Res Commun. 382 Pages: 473-7
  • Peer Reviewed
• [Journal Article] Recombination System Based on Cre alpha Complementation and Leucine Zipper Fusions. (2009)
  • Author(s): Seidi A, Mie M, Kobatake E
  • Journal Title: Appl Biochem Biotechnol. 158 Pages: 334-42
  • Peer Reviewed
• [Journal Article] Construction of nanoscale protein particle using temperature-sensitive elastin-like peptide and polyaspartic acid chain. (2009)
  • Author(s): Fujita Y, Mie M, Kobatake E.
  • Journal Title: Biomaterials 30 Pages: 3450-7
  • Peer Reviewed
• [Journal Article] Transduction of MyoD protein intomyoblasts induces myogenic differentiation without addition of protein transduction domain (2009)
  • Author(s): Noda T, Fujino T, Mie M, Kobatake E
  • Journal Title: Biochem Biophys Res Commun. 382 Pages: 473-477
  • Peer Reviewed
• [Journal Article] Recombination system based on cre alpha complementation and leucine zipper fusions (2009)
  • Author(s): Seidi A, Mie M, Kobatake E
  • Journal Title: Appl Biochem Biotechnol 158 Pages: 334-342
  • Peer Reviewed
• [Journal Article] Construction of nanoscale protein particle using temperature-sensitive elastin-like peptide and polyaspartic acid chain (2009)
  • Author(s): Fujita Y, Mie M, Kobatake E
  • Journal Title: Biomaterials 30 Pages: 3450-3457
  • Peer Reviewed
• [Journal Article] RNA detection using peptide-inserted Renilla luciferase (2009)
  • Author(s): Takashi Andou, Tamaki Endoh, Masayasu Mie, Eiry Kobatake
  • Journal Title: Anal Bioanal Chem 393 Pages: 661-668
  • Peer Reviewed
• [Presentation] 細胞膜透過ドメインを利用した抗体導入法の開発 (2009)
  • Author(s): 小林美紀、三重正和、小畠英理
  • Organizer: 第31回日本バイオマテリアル学会大会
  • Place of Presentation: 京都府民総合交流プラザ・京都テルサ
  • Year and Date: 2009-11-17
• [Presentation] Luciferaseのα-complementationを利用した新規ホモジニアス免疫測定系の開発 (2009)
  • Author(s): ゴファンビッツトゥイ、三重正和、小畠英理
  • Organizer: 第31回日本バイオマテリアル学会大会
  • Place of Presentation: 京都府民総合交流プラザ・京都テルサ
  • Year and Date: 2009-11-17
• [Presentation] Luciferase の α-complementation を利用した新規ホモジニアス免疫測定系の開発 (2009)
  • Author(s): ゴファンビッツトゥイ, 三重正和, 小畠英理
  • Organizer: 第31回日本バイオマテリアル学会大会
  • Place of Presentation: 京都府民総合交流プラザ・京都テルサ
  • Year and Date: 2009-11-17
• [Presentation] 転写因子タンパク質導入による細胞分化誘導 (2009)
  • Author(s): 三重正和、野田智秀、藤野赳至、小畠英理
  • Organizer: 第24回生体機能関連化学シンポジウム・第12回バイオテクノロジー部会シンポジウム
  • Place of Presentation: 九州大学医系キャンパス・百年記念講堂
  • Year and Date: 2009-09-14
• [Presentation] Development of antibody delivery method for protein sensing in living cells (2008)
  • Author(s): Masayasu Mie, Hiromi Kobayashi, Eiry Kobatake
  • Organizer: PRiME 2008 Meeting
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2008-10-16
• [Presentation] Development of RNA Detction System Using Split-Renilla Luciferase (2008)
  • Author(s): Takashi Andou, Tamaki Endoh, Masayasu Mie, Eiry Kobatake
  • Organizer: PRiME 2008 Meeting
  • Place of Presentation: onolulu, Hawaii
  • Year and Date: 2008-10-16
• [Presentation] Development of RNA Detction System Using Split-Renilla Luciferase (2008)
  • Author(s): Takashi Andou, Tamaki Endoh, Masayasu Mie, Eiry Kobatake
  • Organizer: PRiME 2008 Meeting
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2008-10-16