Design of novel transcriptional regulators for the development of an intracellular sensing and functional regulation platform in mammalian cells
| Project/Area Number |
20700389
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
MIKAMI Akane National Institute for Materials Science, 生体材料センター, NIMSポスドク研究員 (70469782)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 生体情報・計測 / 細胞内センシング / 細胞機能制御 / 細胞センシング / 発現制御 / 分子認識 |
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| Research Abstract |
Novel transcriptional regulators (TRs) for a versatile system of intracellular monitoring and transcriptional regulation in mammalian cells have been investigated. Substrate binding proteins and LacI-family TRs chimeric proteins(SLCPs) were designed as novel TRs. SLCPs functioned as TRs in vitro and in mammalian cells, and regulated expression levels of desired genes in a designed target molecule dependent manner, showing their possibility as tools for versatile intracellular sensing/gene regulation systems.
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Report
(3 results)
Research Products
(29 results)
