Development of drug and gene delivery system for injured sites of cardiovascular tissues

• Project/Area Number: 20700396
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: ISE Hirohiko Tokyo Institute of Technology, フロンティア研究センター, 特任講師 (10324253)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 薬物輸送システム / N-アセチルグルコサミン / レクチン / 糖鎖高分子 / ビメンチン / デスミン

Research Abstract

Cardiomyocytes and vascular smooth muscle cells (VSMCs) were found to interact with N-acetylglucosamine (GlcNAc) using glycopolymers containing GlcNAc. It was assumed that these cells have GlcNAc-binding lectin on the cell surfaces. We tried to develop drug and delivery systems for injured site of cardiovascular tissue. The uptake of GlcNAc-conjugated liposomes to cardiomyocytes was observed. These results suggested the development of drug and gene delivery system for injured sites of cardiovascular tissues using GlcNAc-conjugated liposomes.

Research Products

• [Journal Article] Vimentin and desmin possess GlcNAc-binding lectin-like properties on cell surfaces (2010)
  • Author(s): Ise H, Kobayashi S, Goto M, Sato T, Kawakubo M, Takahashi M, Ikeda U, Akaike T.
  • Journal Title: Glycobiology (in press)
  • Peer Reviewed
• [Journal Article] Potential feasibility of early bone marrow cell injection into the spleen for creating functional hepatocytes. (2009)
  • Author(s): Misawa R, Soeda J, Ise H, Takahashi M, Kubota K, Mita A, Nakata T, Miyagawa S
  • Journal Title: Transplantation 87 Pages: 1147-1154
  • Peer Reviewed
• [Journal Article] Surface coating of bone marrow cells with N-acetylglucosamine for bone marrow implantation therapy. (2009)
  • Author(s): Kobayashi S, Ise H, Takahashi M, Goto M, Akaike T, Ikeda U
  • Journal Title: Biomaterials 30 Pages: 574-582
  • Peer Reviewed
• [Journal Article] Potential feasibility of early bone marrow cell injection into the spleen for creating functional hepatocytes (2009)
  • Author(s): Misawa R, Soeda J, Ise H, Takahashi M, Kubota K, Mita A, Nakata T, Miyagawa S.
  • Journal Title: Transplantation 87 Pages: 1147-1154
  • Peer Reviewed
• [Journal Article] Surface coating of bone marrow cells with N-acetylglucosamine for bone marrow implantation therapy (2009)
  • Author(s): Kobayashi S, Ise H, Takahashi M, Goto M, Akaike T, Ikeda U.
  • Journal Title: Biomaterials. 30 Pages: 574-582
  • Peer Reviewed
• [Journal Article] Surface coating of bone marrow cells with N-acetylglucosamine for bone marrow implantation therapy (2009)
  • Author(s): Kobayashi S, Ise H, Takahashi M, Goto M, Akaike T, Ikeda U
  • Journal Title: Biomaterials 30 Pages: 574-582
  • Peer Reviewed
• [Journal Article] Bone marrow CXCR4 induction by cultivation enhances therapeutic angiogenesis (2008)
  • Author(s): Shiba Y, Takahashi M, Hata T, Murayama H, Morimoto H, Ise H. Naaasawa T, Ikeda U
  • Journal Title: Cardiovasc Res 81 Pages: 169-177
  • Peer Reviewed
• [Journal Article] Deficiency of tumour necrosis factor-alpha and interferon-gamma in bone marrow cells synergistically inhibits neointimal formation following vaseular injury (2008)
  • Author(s): Murayama H, Takahashi M, Takamoto M, Shiba Y, Ise H, Koyama J, Tagawa Y, Iwakura Y, Ikeda U
  • Journal Title: Cardiovasc Res 80 Pages: 175-180
  • Peer Reviewed
• [Journal Article] Critical role of bone marrow apoptosis-associated speck-like protein, an inflammasome adaptor molecule, in neointimal formation after vascular injury in mice (2008)
  • Author(s): Yajima N, Takahashi M, Morimoto H, Shiba Y, Takahashi Y, Masumoto J, Ise H, Sagara J, Nakayama J, Taniguchi S, Ikeda U.
  • Journal Title: Circulation 117 Pages: 3079-3087
  • Peer Reviewed
• [Journal Article] MCP-1 induces cardioprotection against ischaemia/reperfusion injury : role of reactive oxygen species (2008)
  • Author(s): Morimoto H, Hirose M, Takahashi M, Kawaguchi M, Ise H, Kolattukudy PE, Yamada M, Ikeda U.
  • Journal Title: Cardiovasc Res 78 Pages: 554-562
  • Peer Reviewed
• [Journal Article] Vimentin and desmin possess GlcNAc-binding lectin-like properties on cell surfaces
  • Author(s): Ise H, Kobayashi S, Goto M, Sato T, Kawakubo M, Takahashi M, Ikeda U, Akaike T
  • Journal Title: Glycobiology (In press)
  • Peer Reviewed
• [Presentation] 人工糖鎖高分子PV-GlcNAcを用いた新規GlcNAc結合性レクチンの探索 (2009)
  • Author(s): 伊勢裕彦、後藤光昭、佐藤孝雄、赤池敏宏
  • Organizer: 第31回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
  • Year and Date: 2009-11-16
• [Presentation] 人工糖鎖高分子PV-GlcNAcを用いた新規GlcNAc結合性レクチンの探索 (2009)
  • Author(s): 伊勢裕彦
  • Organizer: 第31回 日本バイオマテリアル学会大会
  • Place of Presentation: 京都
  • Year and Date: 2009-11-16
• [Presentation] TypeIII型中間径フィラメントのGlcNAc結合レクチン活性と細胞表面の発現 (2009)
  • Author(s): 伊勢裕彦、後藤光昭、佐藤孝雄、赤池敏宏
  • Organizer: 第82回日本生化学大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] Type III型中間径フィラメントのGlcNAc結合レクチン活性と細胞表面の発現 (2009)
  • Author(s): 伊勢裕彦
  • Organizer: 第82回 日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] Rod II domain of vimentin and desmin is expressed on cell surfaces and possesses N-acetylglucosamine-binding lectin-like properties (2009)
  • Author(s): 伊勢裕彦
  • Organizer: 第61回 日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-04
• [Presentation] Development of a drug delivery system using N-acetylglucosamine-conjugated liposomes (2009)
  • Author(s): Ise H, Goto M, Akaike T
  • Organizer: The 7th Asia 3 (China-Japan-Korea) Foresight Symposium on Gene Therapy and Biomaterials
  • Place of Presentation: Korea, Seoul
  • Year and Date: 2009-05-25
• [Presentation] Development of a drug delivery system using N-acetylglucosamine-conjugated liposomes (2009)
  • Author(s): 伊勢裕彦
  • Organizer: The 7^<th> Asia 3(China-Japan-Korea)Foresight Symposium on Gene Therapy and Biomaterials
  • Place of Presentation: Korea, Seoul
  • Year and Date: 2009-05-25
• [Presentation] Development of liver regenerative therapy using glycoside-modified bone marrow cells (2009)
  • Author(s): 伊勢裕彦
  • Organizer: The Okayama 2009 Joint Conference of the 10^<th> biannual Cell Transplant Society congress
  • Place of Presentation: 岡山
  • Year and Date: 2009-04-20
• [Presentation] Novel cell delivery system of bone marrow cells for treatment of myocardial infarction by coating with N-acetylglucosamine on the cell surface (2009)
  • Author(s): 伊勢裕彦
  • Organizer: The Okayama 2009 Joint Conference of the 10^<th> biannual Cell Transplant Society congress
  • Place of Presentation: 岡山
  • Year and Date: 2009-04-20
• [Presentation] 細胞骨格分子であるビメンチン・デスミンの細胞外出現とN-アセチルグルコサミン結合活性 (2008)
  • Author(s): 伊勢裕彦, 他
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 人工的な糖鎖修飾を行った骨髄細胞の心筋組織へのターゲティング法の開発 (2008)
  • Author(s): 小林聡、伊勢裕彦, 他
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 心筋細胞及び血管平滑筋細胞へのN_アセチルグルコサミン修飾リポソームを用いた薬物輸送システムの開発 (2008)
  • Author(s): 伊勢裕彦, 他
  • Organizer: 第46回日本人工臓器学会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-29
• [Presentation] 細胞認識機能性バイオマテリアルの新展開 (2008)
  • Author(s): 赤池敏宏、伊勢裕彦
  • Organizer: 日本バイオマテリアル学会シンポジウム2008
  • Place of Presentation: 東京
  • Year and Date: 2008-11-17
• [Presentation] 人工的な糖鎖修飾を行った骨髄細胞の心筋組織へのターゲティング法の開発 (2008)
  • Author(s): 小林聡、伊勢裕彦, 他
  • Organizer: 日本バイオマテリアル学会シンポジウム2008
  • Place of Presentation: 東京
  • Year and Date: 2008-11-17
• [Presentation] Desmin and vimentin have N-acetylglucosamine-binding lectinic properties and are expressed on the cell surface (2008)
  • Author(s): Hirohiko Ise, et.al.
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-30
• [Book] 医療・診断をめざす先端バイオテクノロジー関根光雄/編 (2009)
  • Author(s): 伊勢裕彦
  • Publisher: 工学図書
• [Book] 医療・診断をめざす先端バイオテクノロジー(関根光雄/編) (2009)
  • Author(s): 伊勢裕彦
  • Publisher: 工学図書