Design of micro-bioanalysis devices using well defined functional surfaces

• Project/Area Number: 20700399
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: NAGASE Kenichi Tokyo Women's Medical University, 医学部, 助教 (10439838)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: インテリジェント材料 / 温度応答性表面 / 相互作用 / 分析 / タンパク質 / 分離

Research Abstract

Bio-analysis systems for analyzing various bioactive compounds such as medicine and pharmaceutical peptides and proteins were developed using poly(N-isopropylacrylamide), temperature-responsive polymer, and its copolymer modified surfaces.

Research Products

• [Journal Article] Thermoresponsive Polymer Brush Surfaces with Hydrophobic Groups for All-Aqueous Chromatography (2010)
  • Author(s): Kenichi Nagase, Mio Kumazaki, Hideko Kanazawa, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Masahiko Annaka, Teruo Okano
  • Journal Title: ACS Applied Materials & Interfaces 2 Pages: 1247-1253
• [Journal Article] Preparation of Thermoresponsive Anionic Copolymer Brush Surfaces for Separating Basic Biomolecules (2010)
  • Author(s): Kenichi Nagase, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Masahiko Annaka, Teruo Okano
  • Journal Title: Biomacromolecules 11 Pages: 215-223
• [Journal Article] Preparation of Thermoresponsive Anionic Copolymer Brush Surfaces forSeparating Basic Biomolecules (2010)
  • Author(s): Kenichi Nagase, Jun Kobayashi Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Masahiko Annaka, Teruo Okano
  • Journal Title: Biomacromolecules 11 Pages: 215-223
  • Peer Reviewed
• [Journal Article] Temperature-responsive intelligent interfaces for biomolecular separationand cell sheet engineering (2009)
  • Author(s): Kenichi Nagase, Jun Kobayashi, Teruo Okano
  • Journal Title: Journal of the Royal Society Interface 6
• [Journal Article] Temperature-responsive inteHigent interfaces for biolnolecular separation and cell sheet engineering (2009)
  • Author(s): Kenichi Nagase, Jun Kobayashi and Teruo Okano
  • Journal Title: Journal of the Royal Society Interface (In press)
  • Peer Reviewed
• [Presentation] Thermoresponsive Anionic Copolymer Brush Surfaces for Adsorption Chromatography (2009)
  • Author(s): Kenichi Nagase, Simuck Yuk, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Masahiko Annaka, Teruo Okano
  • Organizer: 8th International Symposium on Frontiers in Biomedical Polymers
  • Place of Presentation: 東レ総合研修センター(三島)
• [Presentation] Preparation of Temperature-Responsive Anionic Copolymer Brush For Separation of Basic Biomolecules (2009)
  • Author(s): Kenichi Nagase, Simuck Yuk, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Masahiko Annaka, Teruo Okano
  • Organizer: GelSympo 2009
  • Place of Presentation: 関西大学(大阪)
• [Presentation] 温度応答型アニオン性表面の調製とタンパク質の吸脱着制御 (2009)
  • Author(s): 長瀬健一、Yuk Simuck、小林純、菊池明彦、秋山義勝、金澤秀子、安中雅彦、岡野光夫
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本大学(熊本)
• [Presentation] タンパク質精製を目的とした温度応答型アニオン性コポリマーブラシ表面の調製 (2009)
  • Author(s): 長瀬健一、Yuk Simuck、小林純、菊池明彦、秋山義勝、金澤秀子、安中雅彦、岡野光夫
  • Organizer: 第31回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ(京都)
• [Presentation] Thermoresponsive Anionic Copolymer Brush Surfaces for Adsorption Chromatography (2009)
  • Author(s): Kenichi Nagase, Simuck Yuk, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Masahiko Annaka, Teruo Okano
  • Organizer: 8^<th> Intemational Symposiumon Frontiers in Biomedical Polymers
  • Place of Presentation: 東レ総合研修センター(三島)
• [Presentation] Preparation of Temperature-Responsive Anionic Copolymer Brush For Separation of Basic Biomolecules (2009)
  • Author(s): Kenichi Nagase, Simuck Yuk, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Masahiko Annaka, Teruo Okano
  • Organizer: GelSympo2009
  • Place of Presentation: 関西大学(大阪)