Investigation for nutritional substance to enhance skeletal muscle glucose metabolism and the activation effect of AMP kriase

• Project/Area Number: 20700535
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Sports science
• Research Institution: Osaka University of Health and Sport Sciences
• Principal Investigator: HAMADA Tako Osaka University of Health and Sport Sciences, 体育学部, 准教授 (00466294)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: カフェイン / 糖代謝 / 骨格筋 / AMPキナーゼ / 糖尿病 / 栄養補助物質

Research Abstract

The main purpose of this study was to investigate the effect of caffeine as nutritional substance on AMPK activation and glucose metabolism in rat skeletal muscle. It was found that 3mM caffeine increased AMPK phosphorylation. The isoform-specific analysis of the enzyme activity revealed that AMPK α1 and α2 isoforms were simultaneously activated in skeletal muscle. The enzyme activation by caffeine was associated with a reduction in phosphocreatine and an increased glucose uptake. The present findings suggest that caffeine can acutely activate AMPK and increase insulin-independent glucose transport with a reduction in muscle energy status in skeletal muscles. The pharmacological effects of caffeine on risk reduction for diabetes mellitus may be, at least in part, through muscle AMPK activation.

Research Products

• [Journal Article] Morus alba leaf extract stimulates 5'AMP-activated protein kinase in isolated rat skeletal muscle. (2009)
  • Author(s): Ma X, Iwanaka N, Masuda S, Karaike K, Egawa T, Hamada T, Toyoda T, Miyamoto L, Nakao K, Hayashi T.
  • Journal Title: J. Ethnopharmacol. 122 Pages: 54-59
  • Peer Reviewed
• [Journal Article] Caffeine acutely activates 5'adenosine monophosphate-activated protein kinase and increases insulin-independent glucose transport in rat skeletal muscles (2009)
  • Author(s): Egawa T, Hamada T, Kameda N, Karaike K, Ma X, Masuda S, Iwanaka N, Hayashi T.
  • Journal Title: Metabolism 58 Pages: 1609-1617
  • Peer Reviewed
• [Journal Article] Morus alba leaf extract stimulates 5'AMP-activated protein kinase in isolated rat skeletal muscle (2009)
  • Author(s): Ma X, Iwanaka N, Masuda S, Karaike K, Egawa T, Hamada T, Toyoda T, Miyamoto L, Nakao K, Hayashi T.
  • Journal Title: J.Ethnopharmacol. 122 Pages: 54-59
  • Peer Reviewed
• [Journal Article] An association between angiotensin II type 2 receptor gene A/C3123 polymorphism and glycemic control marker in a general Japanese population (2009)
  • Author(s): Kotani K, Fujiwara S, Tsuzaki K, Sano Y, Matsuoka Y, Hamada T, Sakane N.
  • Journal Title: Mol.Biol.Rep. 36 Pages: 917-920
  • Peer Reviewed
• [Journal Article] Adiponectin gene single-nucleotide polymorphisms and treatment response to obesity (2009)
  • Author(s): Tsuzaki K, Kotani K, Nagai N, Saiga K, Sano Y, Hamada T, Moritani T, Yoshimura M, Egawa K, Horikawa C, Kitagawa Y, Kiso Y, Sakane N
  • Journal Title: J.Endocrinol.Investig. 32 Pages: 395-400
  • Peer Reviewed
• [Journal Article] Thermoregulatory sympathetic nervous system activity and diet-induced waist-circumference reduction in obese Japanese women (2009)
  • Author(s): Fujibayashi M, Hamada T, Matsumoto T, Kiyohara N, Tanaka S, Kotani K, Egawa K, Kitagawa Y, Kiso Y, Sakane N, Moritani T.
  • Journal Title: Am.J.Hum.Biol. 21 Pages: 828-835
  • Peer Reviewed
• [Journal Article] Caffeine acutely activates 5'adenosine monophosphate-activated protein kinase and increases insulin-independent glucosetransport in rat skeletal muscles (2009)
  • Author(s): Egawa T, Hamada T, Kameda N, Karaike K, Ma X, Masuda S, Iwanaka N, Hayashi T.
  • Journal Title: Metabolism 58 Pages: 1609-1617
  • Peer Reviewed
• [Journal Article] Low-calorie diet-induced reduction in serum HDL-cholesterol is ameliorated in obese women with the -3826G allele in theuncoupling protein-1 gene (2009)
  • Author(s): Hamada T, Kotani K, Nagai N, Matsuoka Y, Tsuzaki K, Sano Y, Fujibayashi M, Kiyohara N, Tanaka S, Yoshimura Y, Egawa K, Kitagawa Y, Kiso Y, Moritani T, Sakane N.
  • Journal Title: Tohoku.J.Exp.Med. 219 Pages: 337-342
  • Peer Reviewed
• [Journal Article] The common -55 C/T polymorphism in the promoter region of the uncoupling protein 3 gene reduces prevalence of obesity and elevates serum high-density lipoprotein cholesterol levels in the general Japanese population (2008)
  • Author(s): Hamada T, Kotani K, Fujiwara S, Sano Y, Domichi M, Tsuzaki K, Sakane N.
  • Journal Title: Metabolism 57 Pages: 410-415
  • Peer Reviewed
• [Journal Article] The common -55 C/T polymorphism in the promoter region of the uncoupling protein 3 gene reduces prevalence of obesity and elevatesserum high-density lipoprotein cholesterol levels in the general Japanese population. (2008)
  • Author(s): Hamada T, Kotani K, Fujiwara S, Sano Y, Domichi M, Tsuzaki K, Sakane N.
  • Journal Title: Metabolism 57 Pages: 410-415
  • Peer Reviewed
• [Journal Article] The UCP2 -866 A/A genotype is associated with low-density lipoprotein particle sizes in the general population (2008)
  • Author(s): Hamada T, Kotani K, Fujiwara S, Sano Y, Domichi M, Tsuzaki K, Sakane N.
  • Journal Title: Medical Science Monitor 14 Pages: 107-111
  • Peer Reviewed
• [Journal Article] Lack of association of the β_3-adrellergic receptor gelle with energyexpendihlre in response to caffeine in young healthy women. (2008)
  • Author(s): Hamada T, Kotani K, Higashi A, Ikeda J, Tagaki E, Igarashi, A, Matsubara C, Yoshida T, Sakane N.
  • Journal Title: The Tohoku Journal of Experimental Medicine 214 Pages: 365-370
  • Peer Reviewed
• [Journal Article] Lifestyles and oxidative stress in type 2 diabetic patients (2008)
  • Author(s): Kotani K, Sakane N, Tsuzaki K, Matsuoka Y, Sano Y, Hamada T, Yamada K.
  • Journal Title: Scandinavian Journal of Clinical and Laboratory Investigation 8 Pages: 1-3
  • Peer Reviewed
• [Journal Article] The Trp64Arg of β_3-adrenergic receptor gene and predictioll of weight regain in obese female. (2008)
  • Author(s): Sakane N, Hamada T, Kotani N.
  • Journal Title: Obesity Research & Clinical Practice 2 Pages: 69-70
  • Peer Reviewed
• [Journal Article] Oxidative stress, inflammation, and atherosclerotic changes inretinal arteries in the Japanese population ; results from the Mima study. (2008)
  • Author(s): Sakane N, Fujiwara S, Sano Y, Matsuoka Y, Tsuzaki K, Domichi M, Hamada T, Saiga K, Kotani K.
  • Journal Title: Endocrine Journal 55 Pages: 485-488
  • Peer Reviewed
• [Journal Article] The relationship between usual coffee consumption and serum C-reactive protein level in a Japanese female population (2008)
  • Author(s): Kotani K, Tsuzaki K, Sano Y, Maekawa M, Fujiwara S, Hamada T, Sakane N.
  • Journal Title: Clinical Chemistry and Laboratory Medicine 46 Pages: 1434-1437
  • Peer Reviewed
• [Journal Article] フォーミュラ食を用いた減量の効果を規定する要因の検討 (2008)
  • Author(s): 松岡幸代, 永井成美, 佐野喜子, 津崎こころ, 岡崎研太郎, 浜田拓, 田嶋佐和子, 江川香, 北川義徳, 木曽良信, 坂根直樹
  • Journal Title: 肥満研究 14 Pages: 220-225
  • NAID: 10026149661
  • Peer Reviewed
• [Journal Article] Genetic polymorphisms of the renin-angiotensin system and obesity-related metabolic changes in response to low-energy diets in obese women
  • Author(s): Hamada T, Kotani K, Nagai N, Matsuoka Y, Tsuzaki K, Sano Y, Fujibayashi M, Tanaka S, Kiyohara N, Yoshimura Y, Egawa K, Kitagawa Y, Kiso Y, Moritani T, Sakane N.
  • Journal Title: Nutrition (In press)
  • Peer Reviewed
• [Presentation] Mild-restriction diet による減量への抵抗性と代謝関連遺伝子多型の関連 (2009)
  • Author(s): 永井成美, 浜田拓, 坂根直樹, 小谷和彦, 森谷敏夫
  • Organizer: 第30回日本肥満学会
  • Place of Presentation: アクトシティ浜松
  • Year and Date: 2009-10-09
• [Presentation] カフェインの抗糖尿病・催糖尿病作用:骨格筋AMPキナーゼ活性化とインスリンシグナル抑制 (2009)
  • Author(s): 江川達郎, 浜田拓, 林達也
  • Organizer: 第64回同本体力医学会
  • Place of Presentation: 新潟コンベンションセンター
  • Year and Date: 2009-09-18
• [Presentation] カフェインの抗糖尿病・催糖尿病作用:骨格筋AMPキナーゼ活性化とインスリンシグナル抑制 (2009)
  • Author(s): 江川達郎, 浜田拓, 林達也
  • Organizer: 第64回日本体力医学会
  • Place of Presentation: 新潟コンベンションセンター
  • Year and Date: 2009-09-18
• [Presentation] カフェインによるラット骨格筋5'AMP-activated protein kinase(AMPK)α1アイソフォームの優先的活性化 (2009)
  • Author(s): 江川達郎, 浜田拓, 亀田菜央子, 唐池浩平, 馬嘯, 林達也
  • Organizer: 第52回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-05-21
• [Presentation] Caffeine can activat 5'AMP-activated protein kinase and increase insulin-independent giucose uptake in rat skeletal muscles (2008)
  • Author(s): Egawa T, Hamada T, Karaike K, Ma X, Hayashi T.
  • Organizer: 第63回同本体力医学会国際セッション
  • Place of Presentation: 大分別府ビーコンプラザ
  • Year and Date: 2008-09-10
• [Presentation] カフェインによる骨格筋糖代謝と5'AMP-activated protein kinase(AMPK)の活性化効果 (2008)
  • Author(s): 浜田拓, 江川達郎, 唐池浩平, 馬嘯, 林達也
  • Organizer: 第16回日本運動生理学会
  • Place of Presentation: 帝塚山大学
  • Year and Date: 2008-08-02
• [Presentation] カフェインによる骨格筋糖代謝と5'AMP-activated protein kinase(AMPK)の活性化効果 (2008)
  • Author(s): 浜田拓, 江川達郎, 唐池浩平, 馬嘯, 林達也
  • Organizer: 第16回日本運動生理学会
  • Place of Presentation: 手塚山大学
  • Year and Date: 2008-08-02
• [Presentation] Caffeine can activate 5'AMP-activated protein kinase and increase insulin-independent glucose uptake in rat skeletal muscles (2008)
  • Author(s): Hamada T, Egasva T, Karaike K, Ma X, Shimizu Y, Sakane N, Hayashi T.
  • Organizer: American Diabetes Association 68th Scientific Sessions
  • Place of Presentation: SanFraneisco, California
  • Year and Date: 2008-06-06
• [Presentation] The B2 receptor of bradykinin is not essential for the increase in insulin-stimulated glucose uptake following acute exercise (2008)
  • Author(s): Schweitzer GG, Castorena CM, Hamada T, Arias EB, Cartee GD.
  • Organizer: American Diabetes Association 68th Scientific Sessions
  • Place of Presentation: SanFrancisco, California
  • Year and Date: 2008-06-06
• [Presentation] Caffeine can activate 5'AMP-activated protein kinase and increase insulin-independent glucose uptake in rat skeletal muscles. (2008)
  • Author(s): Hamada T, Egawa T, Karaike K, Ma X, Shimizu Y, Sakane N, Hayashi T.
  • Organizer: American Diabetes Association 68th Scientific Sessions
  • Place of Presentation: SanFrancisco, California
  • Year and Date: 2008-06-06
• [Presentation] the B_2 receptor of bradykinin is not essential for the increase in insulin-stimulated glucose uptake following acute exercise (2008)
  • Author(s): Schweitzer GG, Castorena CM, Hamada T, Arias EB, Cartee G D.
  • Organizer: American Diabetes Association 68th Scientific Sessions
  • Place of Presentation: SanFrancisco, California
  • Year and Date: 2008-06-06
• [Presentation] Genetic polymorphisms of the renin-angiotensin system and obesity-related metabolic changes in response to calorie restriction therapy in obese women (2008)
  • Author(s): Hamada T, Kotani K, Sano Y, Nagai N, Moritani T, Sakane N.
  • Organizer: 16th European Congress on Obesity
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 2008-05-14
• [Presentation] Association of angiotensin-converting enzyme DD genotype with diminished thermogenesis in response to acute cold exposure in healthy children (2008)
  • Author(s): Nagai N, Sakane N, Kotani K, Kimura T, Hamada T, Moritani
  • Organizer: 16th European Congress on Obesity
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 2008-05-14
• [Presentation] L-カルニチン含有フォーミュラ食による効果的な減量効果とリバウンド防止に関する研究 (2008)
  • Author(s): 津崎こころ, 小谷和彦, 佐野喜子, 松岡幸代, 浜田拓, 永井成美, 吉村麻紀子, 江川香, 堀川千賀, 北川義徳, 木曽良信, 坂根直樹
  • Organizer: 第62回日本栄養・食糧学会
  • Place of Presentation: 女子栄養大学
  • Year and Date: 2008-05-02