| Project/Area Number |
20700551
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied health science
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| Research Institution | The University of Tokushima |
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Principal Investigator |
MAWATARI Kazuaki The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 講師 (40352372)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAYA Yutaka 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (50136222)
HARADA Nagakatsu 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (40359914)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | モノアミン / 内臓脂肪 / 自発運動 / 肥満 / 脂質 |
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| Research Abstract |
We established a new animal model called SPORTS (Spontaneously-Running Tokushima-Shikoku) rats, which show high-epinephrine (Epi) levels. The aim of this study was to investigate the in vivo effect of Epi on acetyl-CoA carboxylase (ACC) and abdominal visceral fat accumulation. We divided both 6-week male control and SPORTS rats into two groups, which were fed either normal diet or high fat and sucrose (HFS) diet for 16 weeks. Food intake was not different among the groups, but SPORTS rats showed significantly lower weight gain than control rats in both diet groups. After 10 weeks of diet treatment, glucose tolerance tests (GTTs) revealed that SPORTS rats had increased insulin sensitivity. Furthermore, SPORTS rats had lower quantities of both abdominal fat and plasma triglyceride (TG). In abdominal fat, elevated ACC Ser-79 phosphorylation was observed in SPORTS rats and suppressed by an antagonist of β-adrenergic receptor (AR), propranolol, or an inhibitor of AMP-activated protein kinase (AMPK), Compound C. From these results, high level of Epi induced ACC phosphorylation mediated through β-AR and AMPK signaling pathways in abdominal visceral fat of SPORTS rats, which may contribute to reduce abdominal visceral fat accumulation and increase insulin sensitivity. Our results suggest that β-AR-regulated ACC activity would be a target for reating lifestyle-related diseases, such as obesity.
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