Development of instructional method of the tailor-made exercise and diet therapies based on gene polymorphism for improvement of metabolic syndrome

• Project/Area Number: 20700563
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied health science
• Research Institution: International Pacific University
• Principal Investigator: IEMITSU Motoyuki International Pacific University, スポーツ健康科学部, 准教授 (90375460)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 生活習慣病 / 遺伝子多型 / メタボリックシンドローム / 運動 / 食事 / 行動変容

Research Abstract

The present study aimed to identify gene polymorphisms that provided for the behavior modification of the exercise and diet for improvement of metabolic syndrome, and these findings may contribute to basic developing of instructional method of the tailor-made exercise and diet therapies based on gene polymorphism. Firstly, we divided into two groups according to the evaluation of the exercise behavior modification (the physical activity measured by accelerometer) before and after intervention by the exercise guidance for 30-65 years old; the exercise behavior modification group (N=19) and the none exercise behavior modification group (N=13). To investigate comparison analysis of 550,000 gene polymorphisms using gene chip (Human660W-Quad Bead Chip made by the illumina company) between two groups, we have extracted genomic DNA from plasma buffy coats and buccal cells. The result using case-control analysis (p<0.0001) detected 5 SNPs from among 550,000 gene polymorphisms. A gene related to immunological system was included in 5 SNPs. Secondly, we examined to comparison analysis of 550,000 gene polymorphisms by using gene chip among four groups, large or little exercise behavior modification groups before and after intervention by the exercise guidance and high or low physical activity groups through one year (total N=112). The result using case-control analysis (p<0.0001) detected 16 SNPs from among 550,000 gene polymorphisms. Genes related to axon inducement and ion channel in cerebrum were included in 16 SNPs. Thus, these detected SNPs may affect the modification of exercise behavior.

Research Products

• [Journal Article] PPARG genotype accounts for part of individual variation in body weight reduction in response to calorie restriction. (2009)
  • Author(s): Matsuo T, Nakata Y, Katayama Y, Iemitsu M, Maeda S, Okura T, Kim MK, Ohkubo H, Hotta K, Tanaka K.
  • Journal Title: Obesity 17 Pages: 1924-1931
• [Journal Article] Combination of polymorphisms in the beta2-adrenergic receptor and nitric oxide synthase 3 genes increases the risk for hypertension. (2009)
  • Author(s): Misono M, Maeda S, Iemitsu M, Nakata Y, Otsuki T, Sugawara J, Zempo H, Yoshizawa M, Miyaki A, Kuno S, Matsuda M, Ajisaka R.
  • Journal Title: J Hypertens 27 Pages: 1377-1383
• [Journal Article] Poor trunk flexibility is associated with arterial stiffening. (2009)
  • Author(s): Yamamoto K, Kawano H, Gando Y, Iemitsu M, Murakami H, Sanada K, Tanimoto M, Ohmori Y, Higuchi M, Tabata I, Miyachi M.
  • Journal Title: Am J Physiol Heart Circ Physiol 297
• [Journal Article] DHEA improves impaired activation of Akt and PKC zeta/lambda-GLUT4 pathway in skeletal muscle and improves hyperglycaemia in streptozotocin- induced diabetes rats. (2009)
  • Author(s): Sato K, Iemitsu M, Aizawa K, Ajisaka R.
  • Journal Title: Acta Physiol 197 Pages: 217-225
• [Journal Article] Combination of polymorphisms in the beta2-adrenergic receptor and nitric oxide synthase 3 genes increases the risk for hypertension (2009)
  • Author(s): Misono M, Maeda S, Iemitsu M., et al.
  • Journal Title: Journal of Hypertension 27 Pages: 1377-1383
  • Peer Reviewed
• [Journal Article] DHEA improves impaired activation of Akt and PKC zeta/lambda-GLUT4 pathway in skeletal muscle and improves hyperglycaemia in streptozotocin-induced diabetes rats (2009)
  • Author(s): Sato K, Iemitsu M, et al.
  • Journal Title: Acta Physiologica 197 Pages: 217-225
  • Peer Reviewed
• [Journal Article] Poor trunk flexibility is associated with arterial stiffening (2009)
  • Author(s): Yamamoto K, Kawano H, Gando Y, Iemitsu M., et al.
  • Journal Title: American Journal of Physiology Heart and Circulatory Physiology 297
  • Peer Reviewed
• [Journal Article] Relationship between arterial calcification and bone loss in a new combined model rat by ovariectomy and vitamin D(3) plus nicotine. (2008)
  • Author(s): Park JH, Omi N, Iemitsu M, Maeda S, Kitajima A, Nosaka T, Ezawa I.
  • Journal Title: Calcif Tissue Int 83 Pages: 192-201
  • NAID: 120007131200
• [Journal Article] The benefit of medium-chain triglyceride (MCT) therapy on cardiac function of SHR is associated with reversal of metabolic and signaling alterations. (2008)
  • Author(s): Iemitsu M, Shimojo N, Maeda S, Irukayama-Tomobe Y, Sakai S, Ohkubo T, Tanaka Y, Miyauchi T.
  • Journal Title: Am J Physiol Heart Circ Physiol 295
• [Journal Article] Arterial Stiffness, Physical Activity, and Atrial Natriuretic Peptide Gene Polymorphism in Older Subjects. (2008)
  • Author(s): Iemitsu M, Maeda S, Otsuki T, Sugawara J, Kuno S, Ajisaka R, Matsuda M.
  • Journal Title: Hypertens Res 31 Pages: 767-774
  • NAID: 10021934935
• [Journal Article] Testosterone and DHEA activates the glucose metabolism-related signalling pathway in skeletal muscle. (2008)
  • Author(s): Sato K, Iemitsu M, Aizawa K, Ajisaka R.
  • Journal Title: Am J Physiol Endocrinol Metab 294
• [Journal Article] Gender Differences in Brachial Blood Flow during Fatiguing Intermittent Handgrip. (2008)
  • Author(s): Saito Y, Iemitsu M, Otsuki T, Maeda S, Ajisaka R.
  • Journal Title: Med Sci Sports Exer 40 Pages: 684-690
• [Journal Article] Sex differences in steroidogenesis in skeletal muscle following a single bout of exercise in rats. (2008)
  • Author(s): Aizawa K, Iemitsu M, Otsuki T, Maeda S, Miyauchi T, Mesaki N.
  • Journal Title: J Appl Physiol 104 Pages: 67-74
• [Journal Article] Voluntary running exercise attenuates the progression of endothelial dysfunction and arterial calcification in ovariectomized rats. (2008)
  • Author(s): Park JH, Iemitsu M, Maeda S, Kitajima A, Nosaka T, Omi N.
  • Journal Title: Acta Physiol 193 Pages: 47-55
• [Journal Article] Systemic arterial compliance, systemic vascular resistance, and effective arterial elastance during exercise in endurance- trained men. (2008)
  • Author(s): Otsuki T, Maeda S, Iemitsu M, Saito Y, Tanimura Y, Ajisaka R, Miyauchi T.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol 295
• [Journal Article] Estrogen receptor-alpha genotype affects exercise-related reduction of arterial stiffness. (2008)
  • Author(s): Hayashi K, Maeda S, Iemitsu M, Otsuki T, Sugawara J, Tanabe T, Miyauchi T, Kuno S, Ajisaka R, Matsuda M.
  • Journal Title: Med Sci Sports Exer 40 Pages: 252-257
• [Journal Article] Abnormal heart development and lung remodeling in mice lacking the hypoxia-inducible factor-related basic helix-loop-helix PAS protein NEPAS. (2008)
  • Author(s): Yamashita T, Ohneda O, Nagano M, Iemitsu M, Makino Y, Tanaka H, Miyauchi T, Goto K, Ohneda K, Fujii-Kuriyama Y, Poellinger L, Yamamoto M.
  • Journal Title: Mol Cell Biol 28 Pages: 1285-1297
• [Journal Article] Arterial Stiffness, Physical Activity, and Atrial Natriuretic Peptide Gene Polymorphism in Older Subjects (2008)
  • Author(s): Iemitsu M., et.al.
  • Journal Title: Hypertension Research 31 Pages: 767-774
  • NAID: 10021934935
  • Peer Reviewed
• [Journal Article] Estrogen receptor-alpha genotype affects exercise-related reduction of art erial stiffness (2008)
  • Author(s): Hayashi K, Maeda S, Iemitsu M., et.al.
  • Journal Title: Medicine & Science in Sports & Exercise 40 Pages: 252-257
  • Peer Reviewed
• [Journal Article] The benefit of medium-chain triglyceride therapy on the cardiac function of SHRs is associated with a reversal of metabolic and signaling alterations. (2008)
  • Author(s): Iemitsu M., et.al.
  • Journal Title: American Journal of Physiology Heart and Circulatory Physiology 295 Pages: 136-144
  • Peer Reviewed
• [Presentation] 体力レベルの違いが動脈 stiffness とMTHFR遺伝子多型の関係に及ぼす影響 (2009)
  • Author(s): 家光素行
  • Organizer: 第64回日本体力医学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-19
• [Presentation] 体力レベルの違いが動脈stiffnessとMTHFR遺伝子多型の関係に及ぼす影響 (2009)
  • Author(s): 家光素行, 村上晴香, 真田樹義, 山元健太, 河野寛, 丸藤祐子, 谷本道哉, 田畑泉, 宮地元彦
  • Organizer: 第63回日本体力医学会
  • Place of Presentation: 東京
• [Presentation] Physical activity estimated by triaxial accelerometer is an independent predictor of arterial stiffening. (2009)
  • Author(s): Yamamoto K, Kawano H, Gando Y, Iemitsu M, Murakami H, Sanada K, Tanimoto M, Higuchi M, Tabata I, Miyachi M.
  • Organizer: 2009 APS Intersociety Meeting: The Integrative Biology of Exercise V
  • Place of Presentation: South Carolina
• [Presentation] Effects of dietary folate intake and physical activity on the interaction between the plasma homocysteine and MTHFR genotype. (2009)
  • Author(s): Murakami H, Iemitsu M, Yamamoto K, Kawano H, Gando Y, Omori Y, Sanada K, Miyachi M.
  • Organizer: 2009 APS Intersociety Meeting: The Integrative Biology of Exercise V
  • Place of Presentation: South Carolina
• [Presentation] 閉経後の習慣的な運動は心臓の有酸素性エネルギー代謝能とPPARαを介した分子調節を改善させる (2009)
  • Author(s): 家光素行, 朴鍾薫, 佐藤幸治, 前田清司, 麻見直美, 田畑泉
  • Organizer: 第17回日本運動生理学会
  • Place of Presentation: 東京
• [Presentation] Exercise training improves aging-induced decrease of PPAR-alpha and PGC-1alpha protein levels in cardiac and skeletal muscles (2009)
  • Author(s): Iemitsu M, Maeda S.
  • Organizer: 14th Annual congress of the European college of Sports Science
  • Place of Presentation: Oslo, Norway
• [Presentation] トレーニングによる心臓の性ステロイドホルモン濃度の変動 (2008)
  • Author(s): 家光素行, 相澤勝治, 前田清司, 目崎登
  • Organizer: 日本体育学会第59回大会
  • Place of Presentation: 東京
• [Presentation] 中高齢女性におけるalpha-actinin-3遺伝子多型が筋力トレーニング効果に与える影響 (2008)
  • Author(s): 膳法浩史, 田辺解, 村上晴香, 家光素行, 前田清司, 久野譜也
  • Organizer: 日本体育学会第59回大会
  • Place of Presentation: 東京
• [Presentation] スポーツ心肥大の形成機序に関わる細胞内シグナル伝達の調節-MAPKとGSK3βの活性変動- (2008)
  • Author(s): 家光素行, 前田清司
  • Organizer: 第63回日本体力医学会
  • Place of Presentation: 別府
• [Presentation] β2アドレナリン受容体と内皮型NO合成酵素の遺伝子多型の組み合わせは高血圧の発症リスクを増大させる. (2008)
  • Author(s): 美園麻衣子, 前田清司, 家光素行, 中田由夫, 大槻毅, 菅原順, 膳法浩史, 吉澤睦子, 宮木亜沙子, 久野譜也, 松田光生, 鰺坂隆一
  • Organizer: 第63回日本体力医学会
  • Place of Presentation: 別府
• [Presentation] αアクチニン3遺伝子多型がサルコペニアに与える影響 (2008)
  • Author(s): 膳法浩史, 田辺解, 村上晴香, 家光素行, 前田清司, 中野由香利ダニエーラ, 久野譜也
  • Organizer: 第63回日本体力医学会
  • Place of Presentation: 別府
• [Presentation] 血中ホモシステイン濃度とMTHFR遺伝子多型の関連に対する身体活動および葉酸摂取の影響 (2008)
  • Author(s): 村上晴香, 家光素行, 山元健太, 河野寛, 丸藤祐子, 真田樹義, 宮地元彦
  • Organizer: 第63回日本体力医学会
  • Place of Presentation: 別府
• [Presentation] Effects of ACTN3 R577X polymorphism on static torques of knee extensor muscle and leg extensive power in middle aged and older women. (2008)
  • Author(s): Zempo H, Tanabe K, Murakami H, Iemitsu M, Maeda S, Fukunaga T, Kuno S.
  • Organizer: 7th World Congress on Aging and Physical Activity
  • Place of Presentation: Tsukuba, Japan
• [Presentation] The PPAR Gamma Gene, Cardiorespiratory Fitness and Metabolic Syndrome in Young and Old Japanese Men and Women. (2008)
  • Author(s): Sanada K, Miyachi M, Iemitsu M, Murakami H, Tabata I, Yamamoto K, Kawano H, Suzuki K, Higuchi M.
  • Organizer: 13th Annual congress of the European college of Sports Science
  • Place of Presentation: Estoril, Portugal
• [Presentation] Effects of a lifestyle-based physical activity program on metabolic syndrome in Japanese male office workers. (2008)
  • Author(s): Tanabe K, Yokoyama N, Maeda S, Iemitsu M, Sakato Y, Nanba H, Zenpo H, Kuno S.
  • Organizer: 13th Annual congress of the European college of Sports Science
  • Place of Presentation: Estoril, Portugal
• [Presentation] Association of muscle-related gene polymorphisms and thigh muscle cross-sectional area in Japanese postmenopausal women. (2008)
  • Author(s): Zempo H, Tanabe K, Murakami H, Iemitsu M, Maeda, S, Kuno S.
  • Organizer: 13th Annual congress of the European college of Sports Science
  • Place of Presentation: Estoril, Portugal
• [Presentation] Effect of the combination of hypertension-related gene polymorphisms on the risk for hypertension. (2008)
  • Author(s): Misono M, Maeda S, Iemitsu M, Nakata Y, Otsuki T, Sugawara J, Yoshizawa M, Miyaki A, Kuno S, Matsuda M, Ajisaka R.
  • Organizer: 13th Annual congress of the European college of Sports Science
  • Place of Presentation: Estoril, Portugal
• [Book] 『新しい血圧測定と脈波解析マニュアル』第2章2節 : 「運動の効果」 (2008)
  • Author(s): 家光素行, 宮地元彦
  • Publisher: メジカルビュー社