| Project/Area Number |
20700594
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Seinan Jo Gakuin University Junior College |
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Principal Investigator |
TASHIRO Yukihiro Seinan Jo Gakuin University Junior College, 短期大学部・生活創造学科, 准教授 (90448481)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ファージセラビー / ファージセラピー / ビブリオ・バルニフィカス / 細菌性食中毒 / 動物感染モデル |
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| Research Abstract |
A bacteriophage which infects Vibrio vulnificus was searched, and four bacteriophages were isolated from the sediments in Ariake Sea. All isolated bacteriophages could infect only V.vulnificus NBRC 15645^T, which resulted in a quite narrow host specificity. Electron microscopical analysis revealed that the size of bacteriophage No.1 was 200 nm×800 nm. The lysis curve of isolated bacteriophages infecting V.vulnificus NBRC 15645^T were also investigated. Furthermore, a genome extraction from isolated bacteriophages was performed, but the yields were quite low. On the other hand, we gave a hypodermic injection of V.vulnificus ATCC27562^T strain or V. vulnificus K-6 strain to C57BL/J6 mouse's hind leg at the bottom, which succeeded in animal model infected with both V.vulnificus strains. In addition, we also gave a hypodermic injection of K-6 strain or L-K-6 strain, which was lysogenized with its bacteriophage, to six C57BL/J6 mice's hind leg at the bottom. As a result, the injection of K-6 strain killed four of six mice while no mice were killed by L-K-6 strain. Furthermore, L-K-6 strain decreased the growth rate and increase hemolytic activity, compared with K-6 strain.
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