Development of Analytical Procedures for Trace-level Determination of Marine DOC by Mass Spectrometry
| Project/Area Number |
20710017
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
HONGO Yayoi 独立行政法人理化学研究所, 物質構造解析チーム, 技師 (40435681)
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| Project Period (FY) |
2008 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | LC-ESI-MS / 海洋溶存態有機化合物 / 葉酸 / 溶存態有機物 / 光分解 / HILIC / p-aminobenzoly-L-glutamic acid / 溶存有機炭素 / MS / 質量分析 / 海洋化学 / 地球化学 / 微量定量 / 分析化学 |
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| Research Abstract |
Chemical alteration of a compound should be one of the significant factors on the formation of long-lived marine dissolved organic acid. I investigated the three reaction pathways of marine folic acid that is one of the known natural compounds in seawater. To reveal the degradation rate and detect the newly appeared compounds, a seawater incubation experiment with high-performance liquid chromatography electrospray ionization mass spectrometry(LC-ESI-MS) analysis was applied.
The degradation rate of folic acid in the seawater resembled that in the control buffer, suggesting that the marine bacterial contribution was smaller than that of chemical degradation. Photo-irradiation enhanced the degradation rate in both seawater and control medium. However, after 168 h, 67% of folic acid had decomposed without light and marine bacteria, proving that thermal degradation aside from photolysis is important for folic acid degradation. The chemical instability of folic acid was the most probable factor to control the lifetime in seawater. Accurate mass measurement, MS/MS technique and NMR analysis indicated the newly appeared compound in the incubation media as p-aminobenzoylglutamic acid(PAG).
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Report
(6 results)
Research Products
(25 results)
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[Journal Article] Rare earth elements in seawater during an iron-induced phytop Iankton bloom of the western subarctic Pacific(SEEDS-II)2009
Author(s)
Hara, Yasuko, Obata, Hajime, Doi, Takashi, Hongo, Yayoi, Gamo, Toshitaka, Takeda, Shigenobu, Tsuda, Atsushi.
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Journal Title
Deep-Sea Research, Part II : Topical Studies in Oceanography 56
Pages: 2839-2851
Related Report
Peer Reviewed
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[Journal Article] Synthesis of All Possible Isomers Corresponding to the Proposed Structure of Montanacin E, and Their Antitumor Activity2009
Author(s)
Takahashi, Shunya, Takahashi, Ryotaro, Hongo, Yayoi, Koshino, Hiroyuki, Yamaguchi, Kazunori, Miyagi, Taeko
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Journal Title
Journal of Organic Chemistry 74
Pages: 6382-6385
Related Report
Peer Reviewed
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