Long-range transport of halotolerant bacteria in KOSA bioaerosol

• Project/Area Number: 20710024
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental impact assessment/Environmental policy
• Research Institution: Kanazawa University
• Principal Investigator: MAKI Teruya Kanazawa University, 物質化学系, 准教授 (70345601)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 黄砂 / 長距離輸送 / バイオエアロゾル / 極限微生物 / 耐塩細菌 / 健康影響 / 微生物生態 / 大気微生物 / 人健康影響

Research Abstract

The microbial communities transported by Asian desert dust (KOSA) events have attracted much attention as bioaerosols, because the transported microorganisms are thought to influence the downwind ecosystems in Japan. In this study, halotolerant bacteria which are known to tolerant to atmospheric environmental stresses were detected as the long-range transport of microorganisms by KOSA. Bioaerosol samples were collected at high altitudes within the KOSA source area (Dunhuang City, China) and the KOSA arrival area (Suzu City, Japan) belonged to Bacillus subtilis group. Moreover, a bacterial detection technique was established for supporting the monitors of the long-range transport bacteria.

Research Products

• [Journal Article] Phylogenetic analysis of atmospheric halotolerant bacterial communities at high altitude in an Asian dust (KOSA) arrival region, Suzu City (2010)
  • Author(s): T. Maki, S. Susuki, F. Kobayashi, M. Kakikawa, Y. Tobo, M. Yamada, T. Higashi, A. Matsuki, C. Hong, H. Hasegawa, Y. Iwasaka
  • Journal Title: Science of the Total Environment (in press)
  • Peer Reviewed
• [Journal Article] PCR-DGGE analysis of Asian dust (KOSA) bioaerosol recorded in snow cover at Mount Tateyama, Central Japan. (2010)
  • Author(s): T. Maki, K. Aoki, S. Susuki, F. Kobayashi, M. Kakikawa, Y. Tobo, A. Matsuki, H. Hasegawa, Y. Iwasaka
  • Journal Title: Jouranal of Ecotechnology Research (in press)
  • NAID: 130004956089
  • Peer Reviewed
• [Journal Article] バイオエアロゾルのメタゲノム解析, Molecular identification of microorganisms in bioaerosols (2010)
  • Author(s): 柿川真紀子, 岩坂泰信, 小林史尚, 牧輝弥
  • Journal Title: エアロゾル研究 25巻1号 Pages: 29-34
  • Peer Reviewed
• [Journal Article] 能登半島珠洲市上空における黄砂バイオエアロゾルの直接採集および分離培養・同定 (2010)
  • Author(s): 小林史尚, 小谷口久美子, 柿川真紀子, 牧輝弥, 山田丸, 當房豊, 洪天祥, 松木篤, 岩坂泰信
  • Journal Title: エアロゾル研究 25巻1号 Pages: 23-28
  • NAID: 130000252171
  • Peer Reviewed
• [Journal Article] 黄砂バイオエアロゾルに含まれる耐塩細菌群の種組成解析 (2010)
  • Author(s): 牧輝弥, 小林史尚, 柿川真紀子, 鈴木振二, 當房豊, 山田丸, 松木篤, 洪天祥, 長谷川浩, 岩坂泰信
  • Journal Title: エアロゾル研究 25巻1号 Pages: 35-42
  • NAID: 130000252173
  • Peer Reviewed
• [Journal Article] 黄砂バイオエアロゾルに含まれる耐塩細菌群の種組成解析 (2010)
  • Author(s): 牧輝弥, 小林史尚, 柿川真紀子, 鈴木振二, 當房豊, 山田丸, 松木篤, 洪天祥, 長谷川浩, 岩坂泰信
  • Journal Title: エアロゾル研究 25 Pages: 35-42
  • NAID: 130000252173
  • Peer Reviewed
• [Journal Article] 能登半島珠洲市上空における黄砂バイオエアロゾルの直接採集および分離培養・同定 (2010)
  • Author(s): 小林史尚, 小谷口久美子, 柿川真紀子, 牧輝弥, 山田丸, 當房豊, 洪天祥, 松木篤, 岩坂泰信
  • Journal Title: エアロゾル研究 25 Pages: 23-28
  • NAID: 130000252171
  • Peer Reviewed
• [Journal Article] バイオエアロゾルのメタゲノム解析 (2010)
  • Author(s): 柿川真紀子, 岩坂泰信, 小林史尚, 牧輝弥
  • Journal Title: エアロゾル研究 25 Pages: 29-34
  • NAID: 130000252172
  • Peer Reviewed
• [Journal Article] PCR-DGGE analysis of Asian dust (KOSA) bioaerosol recorded in snow cover at Mount Tateyama, Central Japan (2010)
  • Author(s): T.Maki, K.Aoki, S.Susuki, F.Kobayashi, M.Kakikawa, Y.Tobo, A.Matsuki, H.Hasegawa, Y.Iwasaka
  • Journal Title: Jouranal of Ecotechnology Research (掲載確定)
  • NAID: 130004956089
  • Peer Reviewed
• [Journal Article] PCR-DGGE analytical technique of bacterial community in the Lake Kahokugata water spiked with dimetylarsenic acid (2010)
  • Author(s): T.Maki, K.Ueda, W.Hirota, H.Motozima, F.Kobayashi, H.Hasegawa, M.A.Rahman
  • Journal Title: Jouranal of Ecotechnology Research (掲載確定)
  • NAID: 130004956088
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of atmospheric halotolerant bacterial communities at high altitude in an Asian dust (KOSA) arrival region, Suzu City (2010)
  • Author(s): T.Maki, S.Susuki, F.Kobayashi, M.Kakikawa, Y.Tobo, M.Yamada, T.Higashi,A.Matsuki, C.Hong, H.Hasegawa, Y.Iwasaka
  • Journal Title: Science of the Total Environment (掲載確定)
  • Peer Reviewed
• [Journal Article] Seasonal dynamics of biodegradation activities for dimethylarsinic acid (DMA) in Lake Kahokugata (2009)
  • Author(s): T. Maki, W. Hirota, K. Ueda, H. Hasegawa, M.A. Rahman
  • Journal Title: Chemosphere 77(1) Pages: 36-42
  • NAID: 120001613738
  • Peer Reviewed
• [Journal Article] Phylogenetic diversity and vertical distribution of a halobacterial community in the atmosphere of an Asian dust (KOSA) arrival region, Suzu City. (2009)
  • Author(s): T. Maki, S. Susuki, F. Kobayashi, M. Kakikawa, M. Yamada, T. Higashi, A. Matsuki, C. Hong, Y. Tobo, H. Hasegawa, K. Ueda, Y. Iwasaka
  • Journal Title: Jouranal of Ecotechnology Research 14(4) Pages: 255-259
  • NAID: 130004956069
  • Peer Reviewed
• [Journal Article] Mixture of Kosa (Asian dust) and bioaerosols detected in the atmosphere over the Kosa particles source regions with balloon-borne measurements: possibility of long-range transport (2009)
  • Author(s): Y. Iwasaka, G-Y. Shi, M. Yamada, F. Kobayashi, M. Kakikawa, T. Maki, T. Naganuma, B. Chen, Y. Tobo, C. Hong
  • Journal Title: Air Quality, Atmosphere and Health 2(1) Pages: 29-38
  • Peer Reviewed
• [Journal Article] Dustborne microorganisms in the atmosphere over Asian dust (KOSA) source region, Dunhuang. (2009)
  • Author(s): M. Kakikawa, F. Kobayashi, T. Maki, M. Yamada, T. Higashi, B. Chen, G. Shi, C. Hong, Y. Tobo, Y. Iwasaka
  • Journal Title: Air Quality, Atmosphere and Health 1(4) Pages: 195-202
  • Peer Reviewed
• [Journal Article] Seasonal dynamics of biodegradation activities for dimethylarsinic acid (DMA) in Lake Kahokugata (2009)
  • Author(s): T.Maki, W.Hirota, K.Ueda, H.Hasegawa, M.A.Rahman
  • Journal Title: Chemosphere 77 Pages: 36-42
  • NAID: 120001613738
  • Peer Reviewed
• [Journal Article] Hydroxyiminodisuccinic acid (HIDS) : A Novel Biodegradable Chelating Ligand for the Increase of iron bioavailability and arsenic phytoextraction (2009)
  • Author(s): M.A.Rahman, H.Hasegawa, K.Kadohashi, T.Maki, K.Ueda
  • Journal Title: Chemosphere 77 Pages: 207-213
  • NAID: 120001613733
  • Peer Reviewed
• [Journal Article] Seasonal dynamics of bacterial population degrading dimethylarsenic acid in Lake Kahokugata (2009)
  • Author(s): W. Hirota, T. Maki, K. Ueda, T. Shimada, H. Hasegawa, and K. Ueda
  • Journal Title: Jouranal of Ecotechnology Research 14 Pages: 195-200
  • NAID: 130004956058
  • Peer Reviewed
• [Journal Article] Dustborne microorganisms in the atmosphere over Asian dust (KOSA) source region, Dunhuang (2009)
  • Author(s): M. Kakikawa, F. Kobayashi, T. Maki, M. Yamada, T. Higashi, B. Chen, G. Shi, C. Hong, Y. Tobo, Y. Iwasaka
  • Journal Title: Air Quality, Atmosphere and Health (掲載確定)
  • Peer Reviewed
• [Journal Article] Seasonal changes in DMAA-degradation activities in Lake Kahokugata (2009)
  • Author(s): W. Hirota, T. Maki, T. Kakimoto, H. Hasegawa, K. Ueda
  • Journal Title: Jouranal of Ecotechnology Research (掲載確定)
  • NAID: 130004956062
  • Peer Reviewed
• [Journal Article] Mixture of Kosa (Asian dust) and bioaerosols detected. in the atmosphere over the Kosa particles source regions with balloon-borne measurements : possibility of long-range transport (2009)
  • Author(s): Y. Iwasaka, G-Y. Shi, M. Yamada, F. Kobayashi, M. Kakikawa, T. Maki, B. Chen, Y. Tobo, C. Hong
  • Journal Title: Air Quality, Atmosphere and Health 14(掲載確定)
  • Peer Reviewed
• [Journal Article] Phylogenetic diversity and vertical distribution of a halobacterial community in the atmosphere of an Asian dust (KOSA) arrival region, Suzu City (2009)
  • Author(s): T. Maki, S. Susuki, F. Kobayashi, M. Kakikawa, M. Yamada, T. Higashi, A. Matsuki, C. Hong, Y. Tobo, H. Hasegawa, K. Ueda, Y. Iwasaka
  • Journal Title: Jouranal of Ecotechnology Research (掲載確定)
  • NAID: 130004956069
  • Peer Reviewed
• [Journal Article] Phylogenetic diversity and vertical distribution of a halobacterial community in the atmosphere of an Asian dust (KOSA) source region, Dunhuang City. (2008)
  • Author(s): T. Maki, S. Susuki, F. Kobayashi, M. Kakikawa, M. Yamada, T. Higashi, B. Chen, G. Shi, C. Hong, Y. Tobo, H. Hasegawa, K. Ueda, Y. Iwasaka
  • Journal Title: Air Quality, Atmosphere and Health 1 Pages: 81-89
  • Peer Reviewed
• [Journal Article] Effect of iron stress on gene expression in harmful microalga Prymnesium parvum. (2008)
  • Author(s): T. Maki, T. Suzuki, K. Kido, A. Nakahara, T. Higashi, H. Hasegawa, K. Ueda, K. Saijoh
  • Journal Title: Jouranal of Ecotechnology Research (14) Pages: 13-16
  • NAID: 40016271713
  • Peer Reviewed
• [Journal Article] Effect of iron stress on gene expression in harmful microalga Prymnesium parvum (2008)
  • Author(s): T. Maki, T. Suzuki, K. Kido, A. Nakahara, T. Higashi, H. Hasegawa, K. Ueda and K. Saijoh
  • Journal Title: Jouranal of Ecotechnology Research 14 Pages: 13-16
  • NAID: 40016271713
  • Peer Reviewed
• [Journal Article] Bacterial community in the lake water spiked with dimethylarsenic acid estimated by PCR-DGGE analysis (2008)
  • Author(s): T. Shimada, T. Maki, W. Hirota, T. Kakimoto, H. Hasegawa, and K. Ueda
  • Journal Title: Jouranal of Ecotechnology Research 14 Pages: 17-20
  • NAID: 40016271714
  • Peer Reviewed
• [Journal Article] Phylogenetic diversity and vertical distribution of a halobacterial community in the atmosphere of an Asian dust (KOSA) source region, Dunhuang City (2008)
  • Author(s): T. Maki, S. Susuki, F. Kobayashi, M. Kakikawa, M. Yamada, T. Higashi, B. Chen, G. Shi, C. Hong, Y. Tobo, H. Hasegawa, K. Ueda, Y. Iwasaka
  • Journal Title: Air Quality, Atmosphere and Health 1 Pages: 81-89
  • Peer Reviewed
• [Presentation] 能登半島上空で採取した黄砂バイオエアロゾル:PCR-DGGE解析 (2010)
  • Author(s): 牧輝弥
  • Organizer: 第4回大気ハイオエアロゾルシンホジウム
  • Place of Presentation: 名古屋ポートビル
  • Year and Date: 2010-03-14
• [Presentation] 大気バイオエアロゾル中に生残する耐塩細菌群の分散状態 (2010)
  • Author(s): 牧輝弥
  • Organizer: 第4回大気ハイオエアロゾルシンホジウム
  • Place of Presentation: 名古屋ポートビル
  • Year and Date: 2010-03-14
• [Presentation] 三重県沖の海洋調査と黄砂バイオエアロゾルに関する今後の展望 (2010)
  • Author(s): 牧輝弥
  • Organizer: 第4回大気ハイオエアロゾルシンホジウム
  • Place of Presentation: 名古屋ポートビル
  • Year and Date: 2010-03-14
• [Presentation] 沖の海洋調査と黄砂バイオエアロゾルに関する今後の展望 (2010)
  • Author(s): 牧輝弥
  • Organizer: 第4回大気ハイオエアロゾルシンホジウム
  • Place of Presentation: 名古屋,名古屋ポートビル(愛知県)
  • Year and Date: 2010-03-14
• [Presentation] 大気バイオエアロゾル中に生残する耐塩細菌群の分散状態 (2010)
  • Author(s): 牧輝弥
  • Organizer: 第4回大気ハイオエアロゾルシンホジウム
  • Place of Presentation: 名古屋,名古屋ポートビル(愛知県)
  • Year and Date: 2010-03-14
• [Presentation] 能登半島上空で採取した黄砂バイオエアロゾル:PCR-DGGE解析 (2010)
  • Author(s): 牧輝弥
  • Organizer: 第4回大気ハイオエアロゾルシンホジウム
  • Place of Presentation: 名古屋,名古屋ポートビル(愛知県)
  • Year and Date: 2010-03-14
• [Presentation] 黄砂バイオエアロゾルに含まれる核酸塩基配列を用いた微生物の長距離輸送の検証 (2009)
  • Author(s): 牧輝弥
  • Organizer: 京都化学者クラブ234回
  • Place of Presentation: 京都
  • Year and Date: 2009-12-05
• [Presentation] 黄砂バイオエアロゾルに含まれる核酸塩基配列を用いた微生物の長距離輸送の検証-招待講演 (2009)
  • Author(s): 牧輝弥
  • Organizer: 京都化学者クラブ234回
  • Place of Presentation: 京都,京大会館
  • Year and Date: 2009-12-05
• [Presentation] 能登半島上空で採取したバイオエアロゾルの細菌由来核酸断片を用いたPCR-DGGE解析 (2009)
  • Author(s): 鈴木振二, 牧輝弥, 小林史尚, 柿川真紀子, 當房豊, 小林真理子, 長谷川浩, 岩坂泰信
  • Organizer: 第1回能登総合シンポジウム
  • Place of Presentation: 珠洲市珠洲商工会議所会館
  • Year and Date: 2009-11-30
• [Presentation] 能登半島上空で採取したバイオエアロゾルの細菌由来核酸断片を用いたPCR-DGGE解析 (2009)
  • Author(s): 鈴木振二・牧輝弥・小林史尚・柿川真紀子・當房豊・小林真理子・長谷川浩・岩坂泰信
  • Organizer: 第1回能登総合シンポジウム
  • Place of Presentation: 石川県珠洲,珠洲商工会議所会
  • Year and Date: 2009-11-30
• [Presentation] PCR-DGGE analytical technique of bacterial community in the Lake Kahokugata water spiked with dimetylarsenic acid (2009)
  • Author(s): K.Ueda, T.Maki, W.Hirota, M.Hiroyuki, H.Hasegawa, M.A.Rahman
  • Organizer: 16th Asian Symposium on Ecotechnology
  • Place of Presentation: Dalian Neusoft Institute of Information, Dalian, China
  • Year and Date: 2009-10-22
• [Presentation] Phylogenetic diversity ofbioaerosol in the atmosphere of an Asian dust(KOSA)arrival region, Suzu City-招待講演 (2009)
  • Author(s): S.Susuki, T.Maki, F.Kobayashi, M.Kakikawa, M.Yamada, T.Higashi, A.Matsuki, C.Hong, Y.Tobo, H.Hasegawa, Y.Iwasaka
  • Organizer: 16th Asian Symposium on Ecotechnology
  • Place of Presentation: Dalian Neusoft Institute of Information, Dalian, China
  • Year and Date: 2009-10-22
• [Presentation] PCR-DGGE Analysis of Asian Dust (KOSA) Bioaerosol Recorded in Snow Cover at Mount Tateyama, Central Japan (2009)
  • Author(s): T.Maki, S.Susuki, K.Kazuma, F.Kobayashi, M.Kakikawa, H.Hasegawa, Y.Iwasaka
  • Organizer: 16th Asian Symposium on Ecotechnology
  • Place of Presentation: Dalian Neusoft Institute of Information, Dalian, China
  • Year and Date: 2009-10-22
• [Presentation] 能登半島上空で採取したバイオエアロゾルの細菌由来核酸断片を用いたPCR-DGGE解析 (2009)
  • Author(s): 牧輝弥・鈴木振二・小林史尚・柿川真紀子・當房豊・小林真理子・長谷川浩・岩坂泰信
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 札幌 北海道大学学術交流会館
  • Year and Date: 2009-09-25
• [Presentation] 立山積雪断面に含まれる黄砂バイオエアロゾルの核酸塩基配列を用いた細菌組成種組成の解析,日本分析化学会第58年,2009年9月24日-26日,札幌 北海道大 (2009)
  • Author(s): 牧輝弥・鈴木振二・青木一真・小林史尚・柿川真紀子・小林真理子・長谷川浩・岩坂泰信
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 札幌 北海道大学学術交流会館
  • Year and Date: 2009-09-25
• [Presentation] タクラマカン砂漠における大気エアロゾルと挙動をともにする細菌群のPCR-DGGE解析 (2009)
  • Author(s): 鈴木振二, 牧輝弥, 島田孝志, 小林史尚, 柿川真紀子, 山田丸, 當房豊, 東朋美, 長谷川浩, 岩坂泰信
  • Organizer: 第70回分析化学討論会
  • Place of Presentation: 和歌山,和歌山大学
  • Year and Date: 2009-05-17
• [Presentation] 大気バイオエアロゾル中に生残する耐塩細菌群の種組成の解明 (2009)
  • Author(s): 牧輝弥
  • Organizer: 第3回大気ハイオエアロゾルシンホジウム
  • Place of Presentation: 仙台KKRホテル
  • Year and Date: 2009-01-11
• [Presentation] 大気バイオエアロゾル中に生残する耐塩細菌群の種組成の解明 (2009)
  • Author(s): 牧輝弥
  • Organizer: 第3回大気ハイオエアロゾルシンポジウム
  • Place of Presentation: 仙台KKRホテル
  • Year and Date: 2009-01-11
• [Presentation] Comparison of atmospheric bacterial composition in Asian dust (KOSA) source region, Dunhuang city, and arrival region, Suzu city (2009)
  • Author(s): T. Maki, S. Susuki, F. Kobayashi, M. Kakikawa, M. Yamada, T. Higashi, Y. Tobo, A. Matsuki, C. Hong, H. Hasegawa, Y. Iwasaka
  • Organizer: Asian Aerosol Conference AAC09
  • Place of Presentation: Bangkok, Thailand
• [Presentation] PCR-DGGE Analysis of Asian Dust (KOSA) Bioaerosol Recorded in Snow Cover at Mount Tateyama, Central Japan (2009)
  • Author(s): T. Maki, S. Susuki, K. Kazuma, F. Kobayashi, M. Kakikawa, H. Hasegawa, Y. Iwasaka
  • Organizer: 16th Asian Symposium on Ecotechnology
  • Place of Presentation: Dalian Neusoft Institute of Information Dalian, China
• [Presentation] 立山積雪断面に含まれる黄砂バイオエアロゾルの核酸塩基配列を用いた細菌組成種組成の解析 (2009)
  • Author(s): 牧輝弥・鈴木振二・青木一真・小林史尚・柿川真紀子・小林真理子・長谷川浩・岩坂泰信
  • Organizer: 日本分析化学会第58年
  • Place of Presentation: 札幌
• [Presentation] 能登半島上空で採取したバイオエアロゾルの細菌由来核酸断片を用いたPCR-DGGE解析 (2009)
  • Author(s): 牧輝弥・鈴木振二・小林史尚・柿川真紀子・當房豊・小林真理子・長谷川浩・岩坂泰信
  • Organizer: 日本分析化学会第58年
  • Place of Presentation: 札幌
• [Presentation] 珠洲市上空のバイオエアロゾルにおける耐塩細菌群の種組成解析 (2008)
  • Author(s): 牧輝弥, 鈴木振二, 小林史尚, 柿川真紀子, 山田丸, 東朋美, 長谷川浩, 上田一正, 岩坂泰信
  • Organizer: 第24回日本微生物生態学会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-11-26
• [Presentation] 立山積雪断面に含まれる黄砂バイオエアロゾルからの細菌群の検出 (2008)
  • Author(s): 牧輝弥, 鈴木振二, 青木一真, 小林史尚, 柿川真紀子, 山田丸, 東朋美, 長谷川浩, 上田一正, 岩坂泰信
  • Organizer: 日本分析化学会第57年会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-10
• [Presentation] Halotolerant bacterial community survived in atmosphere of Asian dust (KOSA) source region, Dunhuang City (2008)
  • Author(s): T. Maki, S. Susuki, F. Kobayashi, M Kakikawa., M. Yamada, T. Higashi, B. Chen, G. Shi, C. Hong, Y. Tobo, H. Hasegawa, K. Ueda, Y. Iwasaka
  • Organizer: The fifth international workshop on sand and dust storms
  • Place of Presentation: Urumqi, Xinjiang, China
• [Book] 黄砂は微生物を運ぶ箱船か(展望とトピックスp.4, 解説 日本分析化学第58年会) (2009)
  • Author(s): 牧輝弥