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Development of a Technique for Quantitative Analysis of Heavy-ion Induced DNA Double-strand Breaks

Research Project

Project/Area Number 20710047
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionJapan Atomic Energy Agency

Principal Investigator

YOKOTA Yuichiro  Japan Atomic Energy Agency, 量子ビーム応用研究部門, 研究員 (30391288)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords重イオンビーム / 高LET / DNA2本鎖切断 / パルスフィールドゲル電気泳動 / 生物 / 学的効果比 / 限外ろ過 / LET / 電離放射線 / DNA損傷 / ヒト正常線維芽細胞 / 細胞致死効果
Research Abstract

Biological effects of ionizing radiation per dose are larger in high-LET heavy ions than in low-LET radiations. As a reason of this phenomenon, radiation biologists believe that heavy ions induce localized DNA damage which is difficult to repair in irradiated cells. However, the conventional experimental techniques such as pulsed-field gel electrophoresis could not quantify radiation-induced DNA fragmentation that cuts genomic DNA shorter than 10 kbp. In the present study, I have found that most of DNA fragments shorter than 10 kbp were lost during genomic DNA preparation.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (22 results)

All 2010 2009 2008 Other

All Journal Article (5 results) Presentation (16 results) Remarks (1 results)

  • [Journal Article] A quantitative study of DNA double-strand breaks induced by heavy-ion beams : a problem on the conventional DNA-sample preparation.2010

    • Author(s)
      Yuichiro Yokota, Tomoo Funayama, Yasuko Mutou, Tetsuya Sakashita, Michiyo Suzuki, Masahiro Kikuchi, Yasuhiko Kobayashi
    • Journal Title

      JAEA Takasaki Annual Report 2009 87

    • Related Report
      2010 Final Research Report
  • [Journal Article] A quantitative study of DNA double-strand breaks induced by heavy-ion beams : a problem on the conventional DNA-sample preparation.2010

    • Author(s)
      Yuichiro Yokota, et al.
    • Journal Title

      JAEA Takasaki Annual Report

      Pages: 87-87

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Combined treatment of human tumor cells with carbon-ion irradiation and the telomerase inhibitor2009

    • Author(s)
      Yuichiro Yokota et al.
    • Journal Title

      JAEA Takasaki Annual Report

      Pages: 87-87

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Cell-killing effect of heavy ions having different LETs in immortalized human cells.2008

    • Author(s)
      Yuichiro Yokota, Tomoo Funayama, Nobuyuki Hamada, Kana Fukamoto, Tetsuya Sakashita, Masahiro Kikuchi, Michiyo Suzuki, Yasuhiko Kobayashi
    • Journal Title

      JAEA Takasaki Annual Report 2007 89

    • Related Report
      2010 Final Research Report
  • [Journal Article] Cell-killing effect of heavy ions having different LETs in immortalized human cells2008

    • Author(s)
      Yuichiro Yokota
    • Journal Title

      JAEA Takasaki Annual Report

      Pages: 89-89

    • Related Report
      2008 Annual Research Report
  • [Presentation] 重粒子線誘発DNA2本鎖切断定量のための研究開発-パルスフィールドゲル電気泳動を用いた従来の定量法の問題点-2010

    • Author(s)
      横田裕一郎, 他
    • Organizer
      日本放射線影響学会第53回大会
    • Place of Presentation
      京都市、京都テルサ
    • Year and Date
      2010-10-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 重粒子線誘発DNA2本鎖切断定量のための研究開発-パルスフィールドゲル電気泳動を用いた従来の定量法の問題点-2010

    • Author(s)
      横田裕一郎, 他
    • Organizer
      日本放射線影響学会第53回大会
    • Place of Presentation
      京都府 京都市 京都テルサ
    • Year and Date
      2010-10-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 重粒子線誘発DNA2本鎖切断の定量研究:細胞DNA回収における従来法の問題点2010

    • Author(s)
      横田裕一郎, 他
    • Organizer
      第5回高崎量子応用研究シンポジウム
    • Place of Presentation
      高崎市、高崎シティギャラリー
    • Year and Date
      2010-10-15
    • Related Report
      2010 Final Research Report
  • [Presentation] 重粒子線誘発DNA2本鎖切断の定量研究:細胞DNA回収における従来法の問題点2010

    • Author(s)
      横田裕一郎, 他
    • Organizer
      第5回高崎量子応用研究シンポジウム
    • Place of Presentation
      群馬県 高崎市 高崎シティギャラリー
    • Year and Date
      2010-10-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] テロメラーゼ阻害剤MST-312と炭素線の併用によるHeLa細胞の増殖死2009

    • Author(s)
      横田裕一郎, 他
    • Organizer
      日本放射線影響学会第52回大会
    • Place of Presentation
      広島県 広島市 南区民文化センター
    • Year and Date
      2009-11-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 炭素線とテロメラーゼ阻害剤の併用によるヒトがん細胞の細胞致死効果2009

    • Author(s)
      横田裕一郎, 他
    • Organizer
      第4回高崎量子応用研究シンポジウム
    • Place of Presentation
      群馬県 高崎市 高崎シティギャラリー
    • Year and Date
      2009-10-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 重イオンビームによるDNA損傷生成と植物細胞のDNA修復作用2009

    • Author(s)
      横田裕一郎, 他
    • Organizer
      第6回イオンビーム育種研究会大会
    • Place of Presentation
      和光市、理化学研究所鈴木梅太郎ホール
    • Year and Date
      2009-05-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 重イオンビームによるDNA損傷生成と植物細胞のDNA修復作用2009

    • Author(s)
      横田裕一郎, 他
    • Organizer
      第6回イオンビーム育種研究会大会
    • Place of Presentation
      埼玉県 和光市 理化学研究所 鈴木梅太郎ホール
    • Year and Date
      2009-05-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト正常線維芽細胞における重イオンの細胞致死効果2008

    • Author(s)
      横田裕一郎, 他
    • Organizer
      日本放射線影響学会第51回大会
    • Place of Presentation
      北九州市、北九州国際会議場
    • Year and Date
      2008-11-20
    • Related Report
      2010 Final Research Report
  • [Presentation] Cell-killing effect of light- to heavy-ions in normal human fibroblasts.2008

    • Author(s)
      Yuichiro Yokota, et al.
    • Organizer
      The Forth International Symposium on Biomedical Research Using Accelerator Technology
    • Place of Presentation
      前橋市,グリーンドーム前橋
    • Year and Date
      2008-11-17
    • Related Report
      2010 Final Research Report
  • [Presentation] テロメラーゼ触媒サブユニットを過剰発現させたヒト正常線維芽細胞における重イオンビームの細胞致死効果2008

    • Author(s)
      横田裕一郎, 他
    • Organizer
      第3回高崎量子応用研究シンポジウム
    • Place of Presentation
      高崎市、高崎シティギャラリー
    • Year and Date
      2008-10-10
    • Related Report
      2010 Final Research Report
  • [Presentation] ヒト正常線維芽細胞における重イオンビームの細胞致死効果2008

    • Author(s)
      横田裕一郎, 他
    • Organizer
      京都大学原子炉実験所専門研究会
    • Place of Presentation
      大阪府泉南郡、京都大学原子炉実験所
    • Year and Date
      2008-09-10
    • Related Report
      2010 Final Research Report
  • [Presentation] ヒト正常線維芽細胞における重イオンの細胞致死効果2008

    • Author(s)
      横田裕一郎
    • Organizer
      日本放射線影響学会第51回大会
    • Place of Presentation
      北九州市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Cell-killing effect of light- to heavy-ions in normal hiiman fibroblasts2008

    • Author(s)
      Yuichiro Yokota
    • Organizer
      The Forth International Symposium on Biomedica 1 Research Using Accelerator Technology
    • Place of Presentation
      前橋市
    • Related Report
      2008 Annual Research Report
  • [Presentation] テロメラーゼ触媒サブユニットを過剰発現させたヒト正常線維芽細胞における重イオンビームの細胞致死効果2008

    • Author(s)
      横田裕一郎
    • Organizer
      第3回高崎量子応用研究シンポジウム
    • Place of Presentation
      高崎市
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒト正常線維芽細胞における重イオンビームの細胞致死効果2008

    • Author(s)
      横田裕一郎
    • Organizer
      京都大学原子炉実験所専門研究会
    • Place of Presentation
      熊取市
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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