| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
In this study, I tried to develop a stable microflora which can produce ethanol by saccharification of cellulosic wastes. Cellulolytic strains deposited in our laboratory showed a degrading activity to several agrowastes, but they could not make a stable microflora with yeasts. Therefore, I established a new method for screening of cellulolytic microbes and tried to isolate hitherto-uncultured cellulolytic microbes in a soil of temperate zone forest. As a result, various cellulolytic strains including putative novel species were successfully isolated. Most of them possessed xylanase activity as well as cellulose activity. Some isolates could make a stable microflora with yeasts, while only trace amount of ethanol was produced by microbial saccharification and fermentation.
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