Bioactive interface based on electrochemical nano-processing

• Project/Area Number: 20710094
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Tohoku University
• Principal Investigator: KAJI Hirokazu Tohoku University, 大学院・工学研究科, 助教 (70431525)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: バイオチップ / バイオインターフェース / 自己組織化 / 生体材料 / 細胞 / リソグラフィー / パターニング / ナノバイオ / マイクロ・ナノデバイス / AFM / 電気化学

Research Abstract

The electrochemical-based biolithography technique was integrated into an ordinary atomic force microscope (AFM) configuration to pattern proteins and living cells. The patterning procedure could readily be built into an AFM system using a simply fabricated electrochemical AFM probe with an AA-size dry battery. This system enabled us to decrease the pattern size and to image the electrochemically treated surface in situ. Also, in-situ directional control of cell extension was demonstrated.

Research Products

• [Journal Article] Transfer of two-dimensional patterns of human umbilical vein Eendothelial cells into fibrin gels to facilitate vessel formation (2010)
  • Author(s): Takeaki Kawashima, Takeshi Yokoi, Hirokazu Kaji, Matsuhiko Nishizawa
  • Journal Title: Chem. Commun. 46 Pages: 2070-2072
  • Peer Reviewed
• [Journal Article] Transfer of two-dimensional patterns of human umbilical vein Eendothelial cells into fibrin gels to facilitate vessel formation (2010)
  • Author(s): T.Kawashima
  • Journal Title: Chem.Commun. 46 Pages: 2070-2072
  • Peer Reviewed
• [Journal Article] Spatiotemporal subcellular biopatterning using an AFM-assisted electrochemical system (2009)
  • Author(s): Soichiro Sekine, Hirokazu Kaji, Matsuhiko Nishizawa
  • Journal Title: Electrochem. Commun. 11 Pages: 1781-1784
  • Peer Reviewed
• [Journal Article] Selective capture of a specific cell type from mixed leucocytes in an electrode-integrated microfluidic device (2009)
  • Author(s): Masahiko Hashimoto, Hirokazu Kaji, Matsuhiko Nishizawa
  • Journal Title: Biosens. Bioelectron. 24 Pages: 2892-2897
  • Peer Reviewed
• [Journal Article] Controlled cocultures of HeLa cells and human umbilical vein endothelial cells on detachable substrates (2009)
  • Author(s): Hirokazu Kaji, Takeshi Yokoi, Takeaki Kawashima, Matsuhiko Nishizawa
  • Journal Title: Lab Chip 9 Pages: 427-432
  • Peer Reviewed
• [Journal Article] Spatiotemporal subcellular biopatterning using an AFM-assisted electrochemical system (2009)
  • Author(s): S.Sekine
  • Journal Title: Electrochem.Commun. 11 Pages: 1781-1784
  • Peer Reviewed
• [Journal Article] Selective capture of a specific cell type from mixed leucocytes in an electrode-integrated microfluidic device (2009)
  • Author(s): M.Hashimoto
  • Journal Title: Biosens.Bioelectron. 24 Pages: 2892-2897
  • Peer Reviewed
• [Journal Article] Controlled cocultures of HeLa cells and human umbilical vein endothelial cells on detachable substrates (2009)
  • Author(s): H.Kaji
  • Journal Title: Lab Chip 9 Pages: 427-432
  • Peer Reviewed
• [Journal Article] Patterning adherent cells within microchannels by combination of electrochemical biolithography technique and repulsive dielectrophoretic force (2008)
  • Author(s): Hirokazu Kaji, Masahiko Hashimoto, Soichiro Sekine, Takeaki Kawashima, Matsuhiko Nishizawa
  • Journal Title: Electrochemistry 76 Pages: 555-558
  • NAID: 130002150730
  • Peer Reviewed
• [Journal Article] Integration of an electrochemical-based biolithography technique into an AFM system (2008)
  • Author(s): Soichiro Sekine, Hirokazu Kaji, Matsuhiko Nishizawa
  • Journal Title: Anal. Bioanal. Chem. 391 Pages: 2711-2716
  • Peer Reviewed
• [Journal Article] Localized immobilization of proteins onto microstructures within a preassembled microfluidic device (2008)
  • Author(s): Masahiko Hashimoto, Hirokazu Kaji, Maria E. Kemppinen, Matsuhiko Nishizawa
  • Journal Title: Sens. Actuator B-Chem. 128 Pages: 545-551
  • Peer Reviewed
• [Journal Article] In-situリソグラフィーによるバイオチップ表面の動的制御 (2008)
  • Author(s): 梶弘和, 川島丈明, 関根宗一郎, 西澤松彦
  • Journal Title: 表面技術 59 Pages: 371-376
  • NAID: 10021170083
• [Journal Article] Integration of an electrochemical-based biolithography technique into an AFM system (2008)
  • Author(s): S. Sekine
  • Journal Title: Anal. Bioanal Chem. 391 Pages: 2711-2716
  • Peer Reviewed
• [Journal Article] Patterning adherent cells within microchannels by combination of electrochemical biolithography technique and repulsive dielectrophoretic force (2008)
  • Author(s): H. Kaji
  • Journal Title: Electrochemistry 76 Pages: 555-558
  • NAID: 130002150730
  • Peer Reviewed
• [Journal Article] Selective capture of a specific cell type from mixed leucocytes in an electrodes-integrated microfluidic device (2008)
  • Author(s): M. Hashimoto
  • Journal Title: Biosens. Bioelectron 24(印刷中)
  • Peer Reviewed
• [Presentation] バイオインターフェースの時空間制御 (2009)
  • Author(s): 梶弘和(依頼講演)
  • Organizer: 平成20年度東北大学国際高等融合領域研究所セミナー
  • Place of Presentation: 東北大学
  • Year and Date: 2009-01-15
• [Presentation] 電気化学的手法を用いたバイオ界面プロセシング技術の開発と応用開拓 (2009)
  • Author(s): 梶弘和(受賞講演)
  • Organizer: 電気化学会第76回大会
  • Place of Presentation: 京都大学
• [Presentation] In-situ electrochemical processing of conductive polymer film for organic electronic ion pump (2009)
  • Author(s): S.Sekine
  • Organizer: International Symposium on Innovation Nanoscience of Supermolecular Motor Proteins Working in Biomembranes
  • Place of Presentation: Kyoto
• [Presentation] Electrochemical-based surface patterning for biochip application (2008)
  • Author(s): Hirokazu Kaji, Soichiro Sekine, Takeaki Kawashima, Matsuhiko Nishizawa
  • Organizer: The IUMRS International Conference in Asia 2008
  • Place of Presentation: Nagoya, Japan
• [Presentation] Electrochemical bio-lithography at submicron scale for controlling single cell morphology (2008)
  • Author(s): Soichiro Sekine, Hirokazu Kaji, Matsuhiko Nishizawa
  • Organizer: PRiME 2008 Joint International Meeting
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Control of cellular adhesion within microfluidic device using electrochemical biolithography and dielectrophoresis for cell-based assay (2008)
  • Author(s): Masahiko Hashimoto, Hirokazu Kaji, Matsuhiko Nishizawa
  • Organizer: PRiME 2008 Joint International Meeting
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Patterning adherent cells within microchannels by combination of electrochemical biolithography and dielectrophoresis (2008)
  • Author(s): Hirokazu Kaji, Masahiko Hashimoto, Matsuhiko Nishizawa
  • Organizer: TAS 2008
  • Place of Presentation: San Diego, CA, USA
• [Presentation] 電気化学ナノバイオリソグラフィーの開発 (2008)
  • Author(s): 梶弘和, 関根宗一郎, 西澤松彦
  • Organizer: 電気学会センサ・マイクロマシン部門総合研究会
  • Place of Presentation: 仙台
• [Presentation] Integration of electrochemical biolithography into AFM system for manipulating biological molecules (2008)
  • Author(s): Hirokazu Kaji, Soichiro Sekine, Matsuhiko Nishizawa
  • Organizer: Biosensors 2008
  • Place of Presentation: Shanghai, China
• [Presentation] 電気化学ナノバイオリソグラフィーによる細胞形態制御 (2008)
  • Author(s): 関根宗一郎, 梶弘和, 安部隆, 西澤松彦
  • Organizer: 電気化学会第75回大会
  • Place of Presentation: 山梨大学
• [Presentation] Patterning adherent cells within microchannels by combination of electrochemical biolithography and dielectrophoresis (2008)
  • Author(s): H. Kaji
  • Organizer: μTAS 2008
  • Place of Presentation: San Diego, USA
• [Book] "電気化学反応を用いた細胞接着技術"細胞分離・操作技術の最前線(監修:福田敏男, 新井史人) (2008)
  • Author(s): 梶弘和, 西澤松彦
  • Publisher: シーエムシー出版
• [Patent(Industrial Property Rights)] 細胞または組織の培養制御装置とその方法 (2008)
  • Inventor(s): 西澤松彦, 梶弘和, 末永智
  • Industrial Property Rights Holder: 独立行政法人科学技術振興機構
  • Acquisition Date: 2008-10-24