Development of a micro cell sorter with thermal gelation polymer solution on a 3D focusing PDMS chip which fabricated using one step 3D casting technique

• Project/Area Number: 20710103
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: Kazusa DNA Research Institute
• Principal Investigator: SHIRASAKI Yoshitaka Kazusa DNA Research Institute, 免疫ゲノミクス研究グループ, 基礎科学特別研究員 (70469948)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: PDMS / 3次元シースフロー / マイクロセルソーター

Research Abstract

In this research, 3D casting method to fabricate the 3D focusing microchannel and reservoirs in one time has been developed. The 3D focusing microchannel with the multi deck structure which enables to cast in one time has also been developed.The newly developed cell sorting microchip was evaluated with mammalian cells, and the recovery ratio and purity was improved to 100% and over 99% respectively.

Research Products

• [Journal Article] Integration in a multilayer microfluidic chip of 8 parallel cell sorters with flow control by sol-gel transition of thermo- reversible gelation polymer (2010)
  • Author(s): Sugino H, Arakawa T, Nara Y, Shirasaki Y, Ozaki K, Shoji S, Funatsu T
  • Journal Title: Lab Chip (in Press)
  • Peer Reviewed
• [Journal Article] On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation. (2009)
  • Author(s): Sugino H, Ozaki K, Shirasaki Y, Arakawa T, Shoji S, Funatsu T
  • Journal Title: Lab Chip 9(9) Pages: 1254-60
  • Peer Reviewed
• [Journal Article] On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation (2009)
  • Author(s): H. Sugino, K. Ozaki, Y. Shirasaki, T. Arakawa, S. Shoji and T. Funatsu
  • Journal Title: Lob on a chip (RSC Publishing) 9 Pages: 1254-1260
  • Peer Reviewed
• [Journal Article] CELL RUPTURE MICROFLUIDIC-DEVICE USING NANO NEEDLE ALLAY FOR DAMAGE-FREE EXTRACTION OF ORGANELLES (2008)
  • Author(s): T. Arakawa, Y. Shirasaki, D. Yamazaki, T. Funatsu, and S. Shoji
  • Journal Title: The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (micro TAS 2008) Pages: 215-217
  • Peer Reviewed
• [Journal Article] DYNAMIC IMAGING OF SINGLE BIOMOLECULAR INTERACTION USING FLOW CONTROL AND TIRFM (2008)
  • Author(s): T. Arakawa, T. Sameshima, Y. Sato, Y. Sumiyoshi, T. Ueno, Y. Shirasaki, T. Funatsu, and S. Shoji
  • Journal Title: The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (micro TAS 2008) Pages: 296-298
  • Peer Reviewed
• [Journal Article] HIGH PERFORMANCE MULTIPLE BIOMOLECULES SORTING SYSTEM (2008)
  • Author(s): K. Ozaki, H. Sugino, T. Arakawa, Y. Shirasaki, T. Funatsu, and S. Shoji
  • Journal Title: The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (micro TAS 2008) Pages: 489-491
  • Peer Reviewed
• [Journal Article] HIGH PERFORMANCE PARALLEL BIOPARTICLE SORTER WITH 3-DIMENSIONAL PDMS CHIP (2008)
  • Author(s): H. Sugino, Y. Nara, Y. Shirasaki, T. Arakawa, S. Shoji, and T. Funatsu
  • Journal Title: The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (micro TAS 2008) Pages: 1184-1186
  • Peer Reviewed
• [Presentation] オンチップ生体分子ソーターの開発 (2010)
  • Author(s): 白崎善隆
  • Organizer: 日本薬学会第130回年会
  • Place of Presentation: 岡山大学津島キャンパス
  • Year and Date: 2010-03-30
• [Presentation] PDMSチップのワンステップ3次元鋳造加工と温度感受性ハイドロゲルによる哺乳類細胞の分離 (2008)
  • Author(s): 白崎善隆, 荒川貴博, 杉野弘和, 水野潤, 庄子習一, 船津高志, 小原收
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡国際会議場 (福岡)
• [Patent(Industrial Property Rights)] 三次元シースフロー形成構造及び微粒子集束方法 (2009)
  • Inventor(s): 庄子習一、水野潤、後藤峰生、加藤友也、篠原秀敏、船津高志、荒川貴博、小原収、白崎善隆
  • Industrial Property Rights Holder: 財団法人かずさディー・エヌ・エー研究所、学校法人早稲田大学
  • Filing Date: 2009-06-05
• [Patent(Industrial Property Rights)] 三次元シースフロー形成構造及び微粒子集束方法 (2009)
  • Inventor(s): 庄子習一、水野潤、後藤峰生、加藤友也、篠原秀敏、船津高志、荒川貴博、小原牧、白崎善隆
  • Industrial Property Rights Holder: 財団法人かずさデイー・エヌ・エー研究所、学校法人早稲田大学
  • Industrial Property Number: 2009-135665
  • Filing Date: 2009-06-05