RNAi screening for genes involved in differentiation of embryonic stem cells into primordial germ cells.

Research Project

Project/Area Number	20710151
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Applied genomics
Research Institution	Tohoku University
Principal Investigator	MAEDA Ikuma Tohoku University, 加齢医学研究所, 助教 (20376560)
Project Period (FY)	2008 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	生殖細胞 / ゲノム / 発生・分化
Research Abstract	In this study, we utilized mouse embryonic stem cells as an in vitro model for embryogenesis and developed an RNAi screening system for identifying genes involved in development of primordial germ cells. We performed a pilot screen of 864 genes and identified five candidate genes. Knockdown of these genes in ES cells led to a significant increase in expression of germ cell specific genes and these cells seemed to acquire properties of late stage primordial germ cells to some extent.

Report

(3 results)