Proteomics analysis of the chaperonin from intracellular bacterial symbiont

Research Project

Project/Area Number	20710155
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Applied genomics
Research Institution	Japan Agency for Marine-Earth Science and Technology
Principal Investigator	YOSHIDA Takao Japan Agency for Marine-Earth Science and Technology, 海洋・極限環境生物圏領域, チームリーダー (60399566)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	プロテオーム / ゲノム / 進化 / タンパク質 / 蛋白質
Research Abstract	Intracellular sulfur-oxidizing symbiotic bacteria in deep-sea Calyptogena clams are harbored within their gill epithelial cells, and its genomes have been reduced through intracellular association with the hosts. It is possible that the mutations accumulate in the genes. In this study, we examined the expression of molecular chaperone genes including the chaperonin in the symbiont of C. okutanii, and whether the chaperonin functions as buffer of mutation.