| Project/Area Number |
20710161
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Tohoku University |
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Principal Investigator |
SATO Yoko Tohoku University, 大学院・工学研究科, 助教 (40455803)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 膜タンパク質 / トランスロコン / トポロジー / 生体膜 / チャネル |
|---|
| Research Abstract |
The voltage sensors in voltage-dependent potassium channels (Kv channels) consist of the highly positively charged segment, S4, and the negatively charged segments, S2 and S3. How can such highly charged protein domains be efficiently inserted into biological membranes? In this study, we used in vitro translation and translocation experiments to evaluate interactions between residues in the voltage sensor of an archaeal potassium channel, KvAP, and their effect on the final topology in the endoplasmic reticulum (ER) membrane. These results suggest that insertion mechanism of the KvAP is different from plant and insect Kv channels.
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