Network analysis of signal transduction by quantitative phosphoproteomics

• Project/Area Number: 20710168
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Kyushu University
• Principal Investigator: MATSUMOTO Masaki Kyushu University, 生体防御医学研究所, 准教授 (60380531)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: シグナル伝達 / システム生物学 / リン酸化 / プロテオミクス / ネットワーク解析 / システム岸物学

Research Abstract

It is well known that cells expresses various responses to extracellular stimuli, but these mechanisms in which distinct stimuli induces specific response remained poorly understood. To resolve this essential issue concerning signal transduction, I established the system for large scale identification and quantification of protein phosphorylations. Using this system, I obtained quantitative time-resolved information about more than 25,000 phosphorylation sites from several cell lines which were treated with various stimulants.

Research Products

• [Journal Article] Fbxo45, a novel ubiquitin ligase, regulates synaptic activity. (2010)
  • Author(s): Tada, H., Okano, H.J., Takagi, H., Shibata, S., Yao, I., Matsumoto, M., Saiga, T., Nakayama, K. I,. Kashima, H., Takahashi, T., Setou, M., Okano, H
  • Journal Title: J. Biol. Chem. 285(6) Pages: 3840-3849
• [Journal Article] Plasma gelsolin facilitates interaction between beta(2) glycoprotein I and alpha5beta1 integrin. (2009)
  • Author(s): Bohgak, M., Matsumoto, M., Atsumi, T, Kondo, T., Yasuda, S., Horita, T., Nakayama, K.I., Okumura, F., Hatakeyama, S., Koike, T.
  • Journal Title: J Cell Mol Med. (in press)
• [Journal Article] Large-scale proteomic analysis of tyrosine phosphorylation induced by TCR or BCR activation reveals new signaling pathways. (2009)
  • Author(s): Matsumoto, M., Oyamada, K., Takahashi, H., Sato, T., Hatakeyama, S., Nakayama, K. I
  • Journal Title: Proteomics 9 Pages: 3549-3563
• [Journal Article] Fbxo45 forms a novel ubiquitin ligase complex and is required for neuronal development Mol. (2009)
  • Author(s): Saiga, T., Fukuda, T., Matsumoto, M., Tada, H., Okano, H.J., Okano, H., Nakayama KI
  • Journal Title: Cell. Biol. 29 Pages: 3529-3543
• [Journal Article] Nucleolar structure and function are regulated by the deubiquitylating enzyme USP36. (2009)
  • Author(s): Endo, A., Matsumoto, M., Inada, T., Yamamoto, A., Nakayama, K.I., Kitamura, N., Komada, M.
  • Journal Title: J. Cell Sci 122 Pages: 678-686
• [Journal Article] Large-scale proteomic analysis of tyrosine phosphorylation induced by TCR or BCR activation reveals new signaling pathways. (2009)
  • Author(s): Matsumoto, M., Oyamada, K., Takahashi, H., Sato, T., Hatakeyama, S., Nakayama, K.I.
  • Journal Title: Proteomics 9 Pages: 3549-3563
  • Peer Reviewed
• [Journal Article] Fbxo45 forms a novel ubiquitin ligase complex and is required for neuronal development (2009)
  • Author(s): Saiga, T., Fukuda, T., Matsumoto, M., Tada, H., Okano, H.J., Okano, H., Nakayama KI.
  • Journal Title: Mol.Cell.Biol. 29 Pages: 3529-3543
  • Peer Reviewed
• [Journal Article] Nucleolar structure and function are regulated by the deubiquitylating enzyme USP36. (2009)
  • Author(s): Endo, A., Matsumoto, M., Inada, T., Yamamoto, A., Nakayama, K.I., Kitamura, N., Komada, M.
  • Journal Title: J.Cell Sci. 122 Pages: 678-686
  • Peer Reviewed
• [Journal Article] Large-scale proteomic analysis of tyrosine phosphorylation induced by TCR or BCR activation reveals new signaling pathways (2009)
  • Author(s): Matsumoto, M, Oyamada, K., To kahashi, H., Sato, T., Hatakeya ma, S., Nakayama KI.
  • Journal Title: Proteomics (In press)
  • Peer Reviewed
• [Journal Article] Nucleolar structure and function are regulated by the deubiquitylating enzyme USP36 (2009)
  • Author(s): Endo A, Matsumoto M, Inada T, Yamamoto A, Nakayama KI, Kitamura N, Komada M.
  • Journal Title: J Cell Sci 122 Pages: 678-686
  • Peer Reviewed
• [Presentation] 定量リン酸化プロテオミクスによるシグナル伝達の包括的解析 (2009)
  • Author(s): 松本雅記、小山田浩二、中山敬一
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] 定量リン酸化プロテオミクスによるシグナル伝達の包括的解析 (2009)
  • Author(s): 松本雅記
  • Organizer: 蛋白質化学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2009-05-21
• [Presentation] A GLOBAL MAP OF MITOTIC PHOSPHOPROTEOME UNCOVERS U NEXPECTED SIGNALING PATHWAYS IN MITOSIS (2008)
  • Author(s): Masaki Matsumoto, Koji Oyamada, Keiichi I. Nakayama
  • Organizer: Cold spring harbor meeting, The Cell Cycle
  • Place of Presentation: New York, USA
  • Year and Date: 2008-05-15
• [Remarks]
  • URL: http://www.bioreg.kyushu-u.ac.jp/saibou/index.html
• [Remarks]
  • URL: http://www.bioreg.kyushu-u.ac.jp/saibou/index.html
• [Remarks]
  • URL: http://www.bioreg.kyushu-u.ac.jp/saibou/imdex.htm1