Chemical approach for elucidating structure and function of a novel glycolipid stimulating the membrane-protein integration.
| Project/Area Number |
20710174
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Suntory Institute for Bioorganic Research |
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Principal Investigator |
MAEDA Masahide Suntory Institute for Bioorganic Research, 研究員 (80462290)
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| Research Collaborator |
NISHIYAMA Ken-ichi 岩手大学, 農学部付属寒冷バイオフロンティア研究センター, 教授 (80291334)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 天然物有機化学 / 複合糖脂質 / 膜蛋白質輸送 / 構造解析 / 膜蛋白暫輸送 |
|---|
| Research Abstract |
Integration of membrane-proteins into lipid membranes is strictly regulated with translocon, such as SecYEG complex and YidC. Recently, a novel integration-stimulating factor, suggested a sort of glycolipid, was found out in this integration process. In this study, we succeeded in extraction and purification of this factor from E. coli strain MC4100 and assumed a total structure of this integration factor by structural data obtained by NMR and MS analyses.
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Report
(3 results)
Research Products
(2 results)
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[Journal Article] A novel complete reconstitution system for membrane integration of the simplest membrane protein2010
Author(s)
Nishiyama, K., Maeda, M., Abe, M., Kanamori, T., Shimamoto, K., Kusumoto, S., Ueda, T., Tokuda, H.
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Journal Title
Biochem. Biophys. Res. Commun. 394(3)
Pages: 733-736
NAID
Related Report
Peer Reviewed
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