| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
By using in vitro transcription with our unnatural base pair systems, we prepared shRNA molecules containing a series of unnatural bases at a specific position and evaluated their RNAi activities in cultured cells. We found that the activity was retained by replacement with unnatural bases in some specific positions within the passenger and guide strands of the shRNA molecules, and that the tolerance by unnatural-base replacements depended on the size of the unnatural base.
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