Development of Chip Electrophoresis-Laser Spray Ionization Mass Spectrometry Using Gold Nanoparticles

• Project/Area Number: 20750060
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: Kyoto University
• Principal Investigator: KITAGAWA Fumihiko Kyoto University, 工学研究科, 講師 (20362452)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: マイクロチップ電気泳動 / 質量分析 / レーザースプレーイオン化 / ナノスプレー / 金ナノ微粒子 / 局在プラズモン / 熱レンズ顕微鏡 / シクロオレフィンポリマー

Research Abstract

In this study, development of laser spray ionization-mass spectrometry (LSIMS) using gold nanoparticles was investigated on nanospray directly integrated electrophoresis chips. A sample solution containing gold nanoparticles was introduced from the nanospray to the MS detector by the infusion mode. When a 532-nm CW-laser was irradiated to the tip of the nanospray, 12-fold increase in the MS signal intensity was observed in the negative mode. Therefore, it was revealed that LSI-MS using gold nanoparticles gave the MS signal enhancements for anionic analytes.

Research Products

• [Journal Article] Label-free detection of amino acids using gold nanoparticles in electrokinetic chromatography- thermal lens microscopy (2009)
  • Author(s): Kitagawa, F.; Akimoto, Y.; Otsuka, K.
  • Journal Title: J. Chromatogr. A 1216 Pages: 2943-2946
  • Peer Reviewed
• [Journal Article] Label-free detection of amino acids using gold nanoparticles in electrokinetic chromatouranhy-thermal lens microscopy (2009)
  • Author(s): Kitagawa, F.; Akimoto, Y.; Otsuka, K.
  • Journal Title: J. Chromatogr. A 1216 Pages: 2943-2946
  • Peer Reviewed
• [Journal Article] Polymer Microchip Integrated with Nano Electrospray Tip for Electrophoresis-Mass Spectrometry (2008)
  • Author(s): Shinohara, H.; Suzuki, T.; Kitagawa, F.; Mizuno, J.; Otsuka, K.; Shoji, S.
  • Journal Title: Sens. Actuators B 132 Pages: 368-373
  • Peer Reviewed
• [Journal Article] Polymer Microchip Integrated with Nano Electrospray Tip for Electronhoresis-Mass Spectrometry (2008)
  • Author(s): Shinohara, H.; Suzuki, T.; Kitagawa; F.; Mizuno. J.; Otsuka, K.; Shoji, S.
  • Journal Title: Sens. Actuators B 132 Pages: 368-373
  • Peer Reviewed
• [Presentation] Microchip electrophoresis of proteins using microheater integrated PDMS chip. 3 (2010)
  • Author(s): Kana Tanigawa, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 25th International Symposium on Microscale Bioseparations (MSB2010)
  • Place of Presentation: Clarion Congress Hotel Prague, Prague, Czech Republic
• [Presentation] Microchip electrophoresis of proteins using microheater integrated PDMS chip.3 (2010)
  • Author(s): Kana Tanigawa
  • Organizer: 25th International Symposium on Microscale Bioseparations (MSB2010)
  • Place of Presentation: Clarion Congress Hotel Prague, Prague, Czech Republic
• [Presentation] Enhancing the detectability in microscale electrophoretic separations (2009)
  • Author(s): Kenji Sueyoshi, Minako Kai, Kota Hashiba. Fumihiko Kitagawa. Koji Otsuka
  • Organizer: 23rd International Symposium on Microscale Bioseparations (MSB2009 Boston)
  • Place of Presentation: Boston Park Plaza Hotel & Towers, Boston, MA
  • Year and Date: 2009-02-03
• [Presentation] マイクロヒーター集積化PDMSチップを用いる電気泳動分析(4) (2009)
  • Author(s): 谷川佳奈, 末吉健志, 北川文彦, 大塚浩二
  • Organizer: 第29回キャピラリー電気泳動シンポジウム(SCE2009)
  • Place of Presentation: 近畿大学東大阪キャンパス, 東大阪
• [Presentation] マイクロヒーター集積化PDMSチップを用いる電気泳動分析(3) (2009)
  • Author(s): 谷川佳奈, 末吉健志, 北川文彦, 大塚浩二
  • Organizer: 第20回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 金沢エクセルホテル東急, 金沢
• [Presentation] Microchip electrophoresis of proteins using microheater integrated PDMS chip. 2 (2009)
  • Author(s): Kana Tanigawa, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: International Symposium on Microchemistry and Microsystems 2009 (ISMM2009)
  • Place of Presentation: Kanazawa Excel Hotel Tokyu, Kanazawa
• [Presentation] Microchip electrophoresis of proteins using microheater integrated PDMS chip (2009)
  • Author(s): Kana Tanigawa, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 24th International Symposium on Microscale Bioseparations (MSB2009 Dalian)
  • Place of Presentation: Dalian, China
• [Presentation] マイクロヒーター集積化PDMSチップを用いる電気泳動分析(2) (2009)
  • Author(s): 谷川佳奈, 末吉健志, 北川文彦, 大塚浩二
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学, 札幌
• [Presentation] マイクロヒーター集積化PDMSチップを用いる電気泳動分析 (2009)
  • Author(s): 谷川佳奈, 末吉健志, 北川文彦, 大塚浩二
  • Organizer: 第19回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 広島大学東広島キャンパス, 広島
• [Presentation] Enhancing the Detectability in Microscale Electrophoretic Separations (2009)
  • Author(s): Kenji Sueyoshi, Minako Kai, Kota Hashiba, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 23rd International Symposium on Microscale Bioseparations (MSB2009 Boston)
  • Place of Presentation: Boston Park Plaza Hotel & Towers, Boston, MA, USA
• [Presentation] マイクロヒーター集積化PDMSチップを用いる電気泳動分析 (2009)
  • Author(s): 谷川佳奈
  • Organizer: 第19回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 広島大学東広島キャンパス,広島
• [Presentation] マイクロヒーター集積化PDMSチップを用いる電気泳動分析(2) (2009)
  • Author(s): 谷川佳奈
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学,札幌
• [Presentation] Microchip electrophoresis of proteins using microheater integrated PDMS chip.2 (2009)
  • Author(s): Kana Tanigawa
  • Organizer: International Symposium on Microchemistry and Microsystems 2009 (ISMM2009)
  • Place of Presentation: Kanazawa Excel Hotel Tokyu, Kanazawa
• [Presentation] マイクロヒーター集積化PDMSチップを用いる電気泳動分析(3) (2009)
  • Author(s): 谷川佳奈
  • Organizer: 第20回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 金沢エクセルホテル東急,金沢
• [Presentation] マイクロヒーター集積化PDMSチップを用いる電気泳動分析(4) (2009)
  • Author(s): 谷川佳奈
  • Organizer: 第29回キャピラリー電気泳動シンポジウム(SCE2009)
  • Place of Presentation: 近畿大学東大阪キャンパス,東大阪