Direct protein analysis from electrophoresis gel using a mid-infrared laser

• Project/Area Number: 20750062
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: Osaka University
• Principal Investigator: SUZUKI Sachiko Osaka University, 工学研究科, 助教 (20403157)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 生体分析 / 赤外レーザー / 質量分析 / イオン化 / 蛋白質 / 直接分析

Research Abstract

In this study, I performed protein analysis from acrlamide gel after electrophoresis using a Mid-infrared tunable laser. I reported that the IR-MALDI process is influenced by the hydrogen bond around the carboxyl group or residual water. The aclylamide solution has a strong absorption bands at around 6.0μm correstponding to the >C=O stretching vibration mode. I obtained the mass spectrum of insulin ionized from acrylamide gel when the sample was irradiated by the infrared laser in a wavelength range of 5.8-5.9μm.

Research Products

• [Journal Article] 赤外レーザーMALDIの波長依存性に関する研究と応用 (2008)
  • Author(s): 鈴木幸子, 藤田珠美, 粟津邦男
  • Journal Title: Journal of the mass spectrometry society of Japan 56(5) Pages: 235-240
  • Peer Reviewed
• [Journal Article] 赤外レーザーを用いたタンパク質質量分析技術への期待 (2008)
  • Author(s): 鈴木幸子, 粟津邦男
  • Journal Title: OplusE vol30(4) Pages: 348-353
• [Presentation] Development of infrared laser desorption/ionization using polymer film (2009)
  • Author(s): Sachiko Suzuki, Tamami, Fujita, Satoshi Fukumoto
  • Organizer: 18th International Mass SpectrometryConference
  • Place of Presentation: Bremen, Germany
• [Presentation] Proton source of matrix-assisted laser desorption/ionization using an infrared laser (2009)
  • Author(s): Sachiko Suzuki, Tamami, Fujita, Satoshi Fukumoto, Kunio Awazu
  • Organizer: The 57th ASMS Conference on MassSpectrometry
  • Place of Presentation: Phila delphia, PA, USA
• [Presentation] カルボン酸マトリックスを用いたIR-MALDIにおけるプロトン付加分子の生成過程 (2009)
  • Author(s): 鈴木幸子, 藤田珠美, 福本智志, 粟津邦男
  • Organizer: 第57回質量分析総合討論会
  • Place of Presentation: 大阪
• [Presentation] 可視・紫外域透過性高分子化合物シートを用いた赤外レーザーイオン化法の開発 (2009)
  • Author(s): 鈴木幸子, 福本智志
  • Organizer: 第57回質量分析総合討論会
  • Place of Presentation: 大阪
• [Presentation] Proton source of matrix-assisted laser desorption/ionization using an infrared laser (2009)
  • Author(s): Sachiko Suzuki, Tamami,Fujita, Satoshi Fukumoto, Kunio Awazu
  • Organizer: The 57^<th> ASMS Conference on Mass Spectrometry
  • Place of Presentation: Philadelphia, PA, USA
• [Presentation] Development of infrared laser desorption/ionization using polymer film (2009)
  • Author(s): Sachiko Suzuki, Tamami, Fujita, Satoshi Fukumoto
  • Organizer: 18^<th> International Mass Spectrometry Conference
  • Place of Presentation: Bremen, Germany
• [Presentation] Protein analysis from polyacrylamide gel using mid-infrared nanosecond pulsed laser (2008)
  • Author(s): Sachiko Suzuki, Tamami, Fujita, Kunio Awazu
  • Organizer: The 56th ASMS Conference on Mass Spectrometry
  • Place of Presentation: DENVER, Colorado
• [Presentation] 赤外レーザーMALDIの波長依存性に関する研究と応用 (2008)
  • Author(s): 鈴木幸子, 佐藤出, 藤田珠美, 粟津邦男
  • Organizer: 第56回質量分析総合討論会
  • Place of Presentation: つくば
• [Presentation] Protein analysis from polyacrylamide gel using mid-infrared nanosecond pulsed laser (2008)
  • Author(s): Sachiko Suzuki, Tamami, Fujita, Kunio Awazu
  • Organizer: The 56^<th> ASMS Conference on Mass Spectrometry
  • Place of Presentation: DENVER, Colorado