Direct protein analysis from electrophoresis gel using a mid-infrared laser
Project/Area Number |
20750062
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Analytical chemistry
|
Research Institution | Osaka University |
Principal Investigator |
SUZUKI Sachiko Osaka University, 工学研究科, 助教 (20403157)
|
Project Period (FY) |
2008 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 生体分析 / 赤外レーザー / 質量分析 / イオン化 / 蛋白質 / 直接分析 |
Research Abstract |
In this study, I performed protein analysis from acrlamide gel after electrophoresis using a Mid-infrared tunable laser. I reported that the IR-MALDI process is influenced by the hydrogen bond around the carboxyl group or residual water. The aclylamide solution has a strong absorption bands at around 6.0μm correstponding to the >C=O stretching vibration mode. I obtained the mass spectrum of insulin ionized from acrylamide gel when the sample was irradiated by the infrared laser in a wavelength range of 5.8-5.9μm.
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Report
(3 results)
Research Products
(11 results)