Construction of DNA Nanostructures for Single Molecule Expression Analyses

• Project/Area Number: 20750126
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: The University of Tokyo
• Principal Investigator: KUZUYA Akinori The University of Tokyo, 大学院・工学系研究科, 助教 (00456154)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: DNA / ナノ構造体 / タンパク / ナノアレイ / ストレプトアビジン / ナノテクノロジー / DNAナノテクノロジー / DNA Origami / AFM / mRNA / 発現解析 / 選択的切断

Research Abstract

We have designed and prepared a stick-like DNA origami with 9 periodical nanoscale wells that can accommodate exactly one guest molecule such as proteins per well, and successfully prepared streptavidin nanoarrays of desired patterns.

Research Products

• [Journal Article] DNA Origami: Fold, Stick, and Beyond (2010)
  • Author(s): Akinori Kuzuya, Makoto Komiyama
  • Journal Title: Nanoscale 2 Pages: 310-322
  • Peer Reviewed
• [Journal Article] DNA Origami : Fold, Stick, and Beyond (2010)
  • Author(s): KUZUYA, Akinori, KOMIYAMA, Makoto
  • Journal Title: Nanoscale 2 Pages: 310-322
  • Peer Reviewed
• [Journal Article] Asymmetric Secondary and Tertiary Streptavidin/DNA Complexes Selectively Formed in a Nanometer-Scale DNA Well (2010)
  • Author(s): NUMAJIRI, Kentaro, KUZUYA, Akinori, KOMIYMA, Makoto
  • Journal Title: Bioconjugate Chemistry 21 Pages: 338-344
  • Peer Reviewed
• [Journal Article] Precise Site-Selective Termination of DNA Replication by Caging The 3-Position of Thymidine and Its Application to Polymerase Chain Reaction (2009)
  • Author(s): Akinori Kuzuya, Fuminori Okada, Makoto Komiyama
  • Journal Title: Bioconjugate Chem. 20 Pages: 1924-1929
  • Peer Reviewed
• [Journal Article] Design and Construction of a Box-Shaped 3D-DNA Origami (2009)
  • Author(s): Akinori Kuzuya, Makoto Komiyama
  • Journal Title: Chem. Commun. Pages: 4182-4184
  • Peer Reviewed
• [Journal Article] Precisely Programmed and Robust 2D Streptavidin Nanoarray Using Periodical Nanometer-Scale Wells in DNA Origami Assembly (2009)
  • Author(s): Akinori Kuzuya, Mayumi Kimura, Kentaro Numajiri, Naohiro Koshi, Toshiyuki Ohnishi, Fuminori Okada, Makoto Komiyama
  • Journal Title: ChemBioChem 10 Pages: 1811-1815
  • Peer Reviewed
• [Journal Article] Efficient Site-selective RNA Activation and Scission Achieved by Geometry Control of Acridine Intercalation in RNA/DNA Heteroduplex (2009)
  • Author(s): Akinori Kuzuya, Yun Shi, Keita Tanaka, Kenzo Machida, Makoto Komiyama
  • Journal Title: Chem. Lett. 38 Pages: 432-433
  • NAID: 10025121696
  • Peer Reviewed
• [Journal Article] Design and Construction of a Box-Shaped 3D-DNA Origami (2009)
  • Author(s): KUZUYA, Akinori, KOMIYAMA, Makoto
  • Journal Title: Chemical Communications 1 Pages: 4182-4184
  • Peer Reviewed
• [Journal Article] Precisely Programmed and Robust 2D Streptavidin Nanoarrays by Using Periodical Nanometer-Scale Wells Embedded in DNA Origami Assembly (2009)
  • Author(s): KUZUYA, Akinori, 他6名
  • Journal Title: ChemBioChem 10 Pages: 1811-1815
  • Peer Reviewed
• [Journal Article] Effcient Site-selective RNA Activation and Scission Achieved by Geometry Control of Acridine Intercalation in RNA/DNA Heteroduplex (2009)
  • Author(s): KUZUYA, Akinori
  • Journal Title: Chemistry Letters 38 Pages: 432-433
  • Peer Reviewed
• [Journal Article] Single-Molecule Accommodation of Streptavidin in Nanometer-Scale Wells Formed in DNA Nanostructures (2008)
  • Author(s): Akinori Kuzuya, Kentaro Numajiri, Mayumi Kimura, Makoto Komiyama
  • Journal Title: Nucleic Acids Symp. Ser. 52 Pages: 681-682
  • Peer Reviewed
• [Journal Article] Single-Molecule Accommodation of Streptavidin in Nanometer-Scale Wells Formed in DNA Nanostructures (2008)
  • Author(s): KUZUYA, Akinori
  • Journal Title: Nucleic Acids Symposium Series 52 Pages: 681-682
  • Peer Reviewed
• [Presentation] DNA Origamiによる箱形三次元ナノ構造体の作成 (2010)
  • Author(s): 葛谷, 明紀
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学
  • Year and Date: 2010-03-29
• [Presentation] DNA Ohgami による箱形三次元ナノ構造体の作成 (2010)
  • Author(s): 葛谷, 明紀
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学
  • Year and Date: 2010-03-29
• [Presentation] DNA origamiを足場にした機能性ナノマテリアルの選択的固定化 (2009)
  • Author(s): 葛谷, 明紀
  • Organizer: 計測自動制御学会システム・情報部門学術講演会2009
  • Place of Presentation: 東京工業大学
  • Year and Date: 2009-11-26
• [Presentation] DNA origami を足場にした機能性ナノマテリアルの選択的固定化 (2009)
  • Author(s): 葛谷, 明紀
  • Organizer: 計測自動制御学会システム・情報部門学術講演会2009
  • Place of Presentation: 東京工業大学
  • Year and Date: 2009-11-26
• [Presentation] DNAナノ構造体に組み込んだナノメートルサイズのウェルへのゲスト分子の選択的取り込み (2009)
  • Author(s): 葛谷, 明紀
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-18
• [Presentation] DNA origamiによるナノメートルサイズの箱の作製 (2009)
  • Author(s): 葛谷, 明紀
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学
  • Year and Date: 2009-09-14
• [Presentation] DNA origami によるナノメートルサイズの箱の作製 (2009)
  • Author(s): 葛谷明紀
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学
  • Year and Date: 2009-09-14
• [Presentation] ナノメートルサイズのウェルを組み込んだDNA origamiの構築とゲスト分子のサイズ選択的取り込み (2009)
  • Author(s): 葛谷, 明紀
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学
  • Year and Date: 2009-09-13
• [Presentation] ナノメートルサイズのウェルを組み込んだDNA origami の構築とゲスト分子のサイズ選択的取り込み (2009)
  • Author(s): 葛谷明紀
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学
  • Year and Date: 2009-09-13
• [Presentation] Streptavidin nanoarray formed on a tape-like DNA nanostructure equipped with nanometer-sized wells (2009)
  • Author(s): KUZUYA, Akinori
  • Organizer: FNANO2010
  • Place of Presentation: Snowbird, Utah, USA
  • Year and Date: 2009-04-22
• [Presentation] ナノメートルサイズのウェルを複数組み込んだDNA Origamiの構築とタンパクナノアレイ作成への応用 (2009)
  • Author(s): 葛谷, 明紀
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学理工学部船橋キャンパス
  • Year and Date: 2009-03-30
• [Presentation] DNAで作ったナノメートル空問内におけるストレプトアビジンの選択的DNA修飾 (2009)
  • Author(s): 沼尻, 健太郎
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学理工学部船橋キャンパス
  • Year and Date: 2009-03-30
• [Presentation] DNAで作ったナノメートル空間内におけるストレプトアビジンの選択的DNA修飾 (2008)
  • Author(s): 沼尻, 健太郎
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学理工学部
  • Year and Date: 2008-03-30
• [Presentation] ナノメートルサイズのウェルを複数組み込んだDNA Origamiの構築とタンパクナノアレイ作成への応用 (2008)
  • Author(s): 葛谷, 明紀
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学理工学部
  • Year and Date: 2008-03-30
• [Remarks]
  • URL: http://www.mkomi.rcast.u-tokyo.ac.jp/member/kuzuya.htm