Photoswitching of RNA Function by using photoresponsive RNA
Project/Area Number |
20750132
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Chemistry related to living body
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Research Institution | Nagoya University |
Principal Investigator |
LIANG Xingguo Nagoya University, 工学研究科, 准教授 (60447828)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | RNA / アゾベンゼン / 光制御 / 光スイッチング / リボザイム / 光応答性RNA / RNA/RNA二重鎖 / siRNA / RNAi / 遺伝子発現 / RNAハイブリダイゼション / RNA二重鎖 |
Research Abstract |
By chemically modifying RNA with azobenzene moieties, whose molecular structures change greatly with light irradiation, photoresponsive RNA is prepared. By using the photoisomerization of introduced azobenzene, formation and dissociation of RNA/RNA and RNA/DNA duplex has been photoregulated. Furthermore, multiple azobenzenes have been introduced to a ribozyme with enzymatic activity, and photoswitching of RNA cleavage at a specific site has been realized. The photoresponsive RNA is promising to be used for photoswitching RNA function.
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Report
(4 results)
Research Products
(98 results)
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[Journal Article] Robust and photo-controllable DNA capsules using azobenzenes.2010
Author(s)
Tanaka, F. ; Mochizuki, T. ; Liang, X.G. ; Asanuma, H. ; Tanaka, S. ; Suzuki, K. ; Kitamura, S. ; Nishikawa, A. ; Ui-Tei, K. ; Hagiya, M.
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Journal Title
Nano Lett. 10
Pages: 3560-3565
Related Report
Peer Reviewed
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[Journal Article]2010
Author(s)
Hara, Y. ; Fujii, T. ; Kashida, H. ; Sekiguchi, K. ; Liang, X.G. ; Niwa, K. ; Takase, T. ; Yoshida, Y. ; Asanuma, H.
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Journal Title
Angew.Chem.Int.Ed. 49
Pages: 5502-5506
Related Report
Peer Reviewed
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