| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
Although the NQ-photosensitized oxidation accompanied by selective strand cleavage at ^mC in DNA is an attractive method for identification of the methylation site in DNA, the relatively lower sensitivity of the detection method due to the less efficiency in the photooxidation of ^mC still remains as a major subject to be improved for establishing a status of general availability. In this study, we characterized reaction mechanism of the NQ-photosensitized one-electron oxidation of 5-methyl-2-deoxycytidine (d^mC) in order to obtain insight into the design of photochemical system involving the ^mC-selective strand cleavage with high sensitivity. We confirmed that deprotonation of d^mC radical cation and formation of methyl radical species are key reaction for the present photoreaction.
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