Identification of protein dynamics for enzymatic reaction using four-dimensional visualizing analysis
Project/Area Number |
20750137
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Chemistry related to living body
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Research Institution | Osaka University |
Principal Investigator |
NOJIRI Masaki Osaka University, 大学院・理学研究科, 助教 (20333346)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | X線結晶構造解析 / 酵素反応機構 / 時間分解 / 生体分子 / 酵素反応 / 酵素 / X線 / 結晶構造解析 |
Research Abstract |
The objective for this research project is to establish the methods for visualizing the protein dynamics on enzymatic reaction mechanism. In this case, we selected a crystal for copper-containing nitrite reductase as a test case, and collected the diffraction data during high-brilliant x-ray induced reduction. As a result, we have succeeded in observation of some unambiguous structural changes at the copper binding sites in the difference fourier electron density maps.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of GK0767, the copper-containing nitrite reductase from Geobacillus kaustophilus.2011
Author(s)
Fukuda,Y., Tamada,T., Takami,H., Suzuki,S., Inoue,T., Nojiri,M
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Journal Title
Acta Cryst., F (in press)
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[Journal Article] Structural basis of inter-protein electron transfer for nitrite reduction in denitrification.2009
Author(s)
Nojiri,M., Koteishi,H., Nakagami,T., Kobayashi,K., Inoue,T., Yamaguchi,K., Suzuki,S
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Journal Title
Nature 462
Pages: 117-117
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[Journal Article] Cytochrome c551 is a mediator of electron transfer between copper-containing nitrite reductase and azurin in a denitrifying bacterium, Achromobacter xylosoxidans.2009
Author(s)
Koteishi,H., Nojiri,M., Nakagami,T., Yamaguchi,K., Suzuki,S
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Journal Title
Bull.Chem.Soc.Jpn. 82
Pages: 1003-1003
NAID
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[Journal Article] Expression, purification, crystallization and preliminary X-ray diffraction analysis of the soluble domain of PPA0092, a putative nitrite reductase from Propionibacterium acnes.2009
Author(s)
Nojiri,M., Shirota,F., Hira,D., Suzuki,S
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Journal Title
Acta Cryst. F65
Pages: 123-123
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[Journal Article] The first example of photochemical reduction of nitrite into nitrogen monoxide by a dinuclear Ru(II)-Cu(II) complex and photoinduced intramolecular electron transfer reaction between Ru(II) and Cu(II) moieties.2009
Author(s)
Isoda,N., Torii,Y., Okada,T., Misoo,M., Yokoyama,H., Ikeda,N., Nojiri,M., Suzuki,S., Yamaguchi,K
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Journal Title
Dalton Trans. 46
Pages: 10175-10175
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[Journal Article] Electrocatalytic nitrite reduction to nitrogen oxide by a synthetic analogue of the active site of Cu-containing nitrite reductase incorporated in nafion film.2009
Author(s)
Migita,Y., Yokoyama,H., Minami,A., Mori,T., Nojiri,M., Suzuki,S., Yamaguchi,K
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Journal Title
Electroanalysis 21
Pages: 2441-2441
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[Journal Article] Pulse radiolysis of hexameric nitrite reductase containing the type 1 Cu sites in a monomer.2008
Author(s)
Suzuki,S., Maetani,T., Nojiri,M., Yamaguchi,K., Kobayashi,K., Tagawa,S
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Journal Title
Bull.Chem.Soc.Jpn. 81
Pages: 1525-1525
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[Presentation] Role of the N-terminal cupredoxin domain of a hexameric nitrite reductase2010
Author(s)
Ikebuchi, S., Koteishi, H., Yamaguchi, K., Suzuki, S., Nojiri, M.
Organizer
The International Chemical Congress of Pacific Basin Societies
Place of Presentation
Hawaii, USA
Year and Date
2010-12-15
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[Presentation] Inter- and intra-molecular complex structures of Cu-containing nitrite reductase with cytochrome c.2008
Author(s)
Nojiri, M., Koteishi, H., Tsuda, A., Yamaguchi, K., Suzuki, S.
Organizer
XXI IUCr Congress
Place of Presentation
Grad Cube Osaka, Japan
Year and Date
2008-08-23
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