Development of a molecule-dependent protein expression system that is different from natural riboswitches and its application to biosensors

• Project/Area Number: 20750145
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Ehime University
• Principal Investigator: OGAWA Atsushi Ehime University, 上級研究員センター, 上級研究員 (30442940)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 生体機能関連化学 / 核酸 / バイオテクノロジー / 発現制御 / 生体機能利用

Research Abstract

Two novel gene regulation systems that are controlled by specific molecules have been constructed. One works in a prokaryotic translation system and the other does in a eukaryotic one. These systems are biological ones and target molecules can be changed with simple adjustments, so that they are expected to be used as versatile biosensors both in vitro and in vivo.

Research Products

• [Journal Article] Biofunction-Assisted Sensors Based on a New Method for Converting Aptazyme Activity into Reporter Protein Expression with High Efficiency in Wheat Germ Extract (2009)
  • Author(s): Atsushi Ogawa
  • Journal Title: ChemBioChem 10 Pages: 2465-2468
  • Peer Reviewed
• [Journal Article] Biofunction-Assisted Sensors Based on a New Method for Converting A ptazyme Activity into Reporter Protein Expression with High Efficiency in Wheat Germ Extract (2009)
  • Author(s): Atsushi Ogawa
  • Journal Title: ChemBioChem 10 Pages: 2465-2468
  • Peer Reviewed
• [Journal Article] Aptazyme-based biosensors using a eukaryotic cell-free translation system (2009)
  • Author(s): Atsushi Ogawa
  • Journal Title: Nucleic Acids Symp.Ser. 53 Pages: 261-262
  • Peer Reviewed
• [Journal Article] A Novel Label-Free Biosensor Using an Aptazyme-Suppressor-tRNA Conjugate and an Amber Mutated Reporter Gene (2008)
  • Author(s): Atsushi Ogawa、Mizuo Maeda
  • Journal Title: ChemBioChem 9 Pages: 2204-2208
  • Peer Reviewed
• [Journal Article] A Novel Label-Free Biosensor Using an Aptazyme/Suppressor-tRNA Conjugate and an Amber-Mutated Reporter Gene (2008)
  • Author(s): Atsushi Ogawa, Mizuo Maeda
  • Journal Title: ChemBioChem 9 Pages: 2204-2208
  • Peer Reviewed
• [Presentation] 真核生物の無細胞翻訳システムを利用したアプタザイム基盤バイオセンサー (2010)
  • Author(s): 小川敦司
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 東大阪
  • Year and Date: 2010-03-28
• [Presentation] 真核生物の無細胞翻訳システムを利用したアプタザイム基盤バイオセンサー (2010)
  • Author(s): 小川敦司
  • Organizer: 日本化学会第90春季年会(2010)
  • Place of Presentation: 東大阪
  • Year and Date: 2010-03-28
• [Presentation] Aptazyme-Based Biosensors Using a Eukaryotic Cell-Free Translation System (2009)
  • Author(s): Atsushi Ogawa
  • Organizer: The 6th International symposium on Nucleic Acid Chemistry
  • Place of Presentation: 高山
  • Year and Date: 2009-09-29
• [Presentation] 無細胞翻訳システムを用いた新しいバイオテクノロジーの開発 (2009)
  • Author(s): 小川敦司
  • Organizer: 新世代の生物有機化学研究会2009
  • Place of Presentation: 京都
  • Year and Date: 2009-05-30
• [Presentation] Molecule-Dependent Gene Regulation (2009)
  • Author(s): 小川敦司
  • Organizer: 3^<rd> Luncheon Seminar 2009
  • Place of Presentation: 松山
  • Year and Date: 2009-04-13
• [Presentation] 分子応答性タンパク質発現システムの開発 (2009)
  • Author(s): 小川敦司
  • Organizer: 第3回無細胞生命科学研究会
  • Place of Presentation: 弘前
  • Year and Date: 2009-03-17
• [Presentation] 分子応答性タンパク質発現システムの開発 (2009)
  • Author(s): 小川敦司、前田瑞夫
  • Organizer: 第3回無細胞生命科学研究会
  • Place of Presentation: 弘前(弘前大)
  • Year and Date: 2009-03-17
• [Presentation] 機能性核酸アプタザイムを用いた新規遺伝子発現制御法・分子検出法の開発 (2008)
  • Author(s): 小川敦司
  • Organizer: 第37回環境分子科学研究会
  • Place of Presentation: 和光(理研)
  • Year and Date: 2008-10-07
• [Presentation] サプレッサーtRNA基盤リボスイッチシステムの開発 (2008)
  • Author(s): 小川敦司
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-09-18
• [Presentation] サプレッサーtRNA基盤リボスイッチシステムの開発 (2008)
  • Author(s): 小川敦司、前田瑞夫
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 東京(東工大)
  • Year and Date: 2008-09-18
• [Presentation] アプタザイムを利用した小分子検出システムの開発 (2008)
  • Author(s): 小川敦司
  • Organizer: 新世代の生物有機化学研究会2008
  • Place of Presentation: 名古屋
  • Year and Date: 2008-06-28